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161.
162.
Activity-dependent neuroprotective protein (ADNP) 2 (KIAA0863; ZNF508) gene, a homeobox-profile containing gene, was identified in a screen for homologous proteins to ADNP. The human ADNP2 contains 1131 amino acid residues with a molecular weight of 122.8 KDa. In silico analysis indicated that ortholgs to ADNP2 exist in different phyla, suggesting that ADNP2 might be evolutionary conserved. Here, we began to explore the molecular and functional characterization of ADNP2. Results showed that the mouse ADNP2 mRNA is ubiquitously expressed in distinct normal tissues with increased expression in the brain, particularly in the cerebral cortex. During development, a relatively high level of ADNP2 gene expression was found in the embryonic mouse brain and was sustained throughout embryogenesis and adulthood. An increase in the mRNA was detected in differentiated P19 neuronal/glial-like cells as compared with the non-differentiated cells. To gain insight into ADNP2 function, ADNP2-deficient cell lines were established by the RNA silencing (small interfering RNA) technology. ADNP2 deficiency significantly changed the toxicity induced by hydrogen peroxide in P19 embryonic carcinoma cells, similar to what would be predicted for ADNP deficiency. These findings represent an initial characterization of ADNP2 and suggest that this gene product may have an important function in brain by playing a role in cellular survival pathways. 相似文献
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The Accelerated Failure Time Model Under Biased Sampling 总被引:1,自引:0,他引:1
Summary Chen (2009, Biometrics) studies the semi‐parametric accelerated failure time model for data that are size biased. Chen considers only the uncensored case and uses hazard‐based estimation methods originally developed for censored observations. However, for uncensored data, a simple linear regression on the log scale is more natural and provides better estimators. 相似文献
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M. Maitre J.M. Blindermann L. Ossola P. Mandel 《Biochemical and biophysical research communications》1978,85(3):885-890
Human and rat L-glutamate decarboxylases have been purified to electrophoretic homogeneity. These two enzymes were compared using an immunochemical method, amino acid analysis and tryptic fingerprinting. Structural studies revealed several differences in the primary structure of the two enzymes, but the immunochemical method used did not distinguish between the antigenicity of the two proteins. 相似文献
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169.
Indoleamine-N-methyltransferase from rabbit and human lung catalyzes the N-methylation of 5-methoxytryptamine to form 5-methoxy-N-methyltryptamine, which in turn, is converted to 5-methoxy-N,N-dimethyltryptamine. S-Adenosylmethionine is the methyl donor for these reactions. There was no evidence for the 0-methylation of bufotenin by either S-adenosylmethionine or 5-methyltetrahydrofolic acid using enzymes from rabbit lung, rat brain and liver, or chick heart. 相似文献
170.
Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity 总被引:15,自引:0,他引:15
Rat preprosomatostatin (rPPSS) is processed to two bioactive peptides, somatostatin-14 and somatostatin-28. In anglerfish islets, the two peptides are synthesized by distinct cell types and are derived from different precursors, anglerfish preprosomatostatin-1 (a(I)PPSS) and anglerfish preprosomatostatin-2 (a(II)PPSS). To determine the basis of the differential processing, we introduced a(I)PPSS or a(II)PPSS expression vectors into mammalian endocrine cell lines that can accomplish both patterns of processing. Both precursors were processed identically, indicating that cellular factors must determine the processing pattern. Although similar processing sites are present in both precursors, high levels of unprocessed anglerfish prosomatostatin-2 were secreted constitutively from the transfected cells. A hybrid protein containing the leader sequence and a portion of the pro-region of rPPSS fused to the carboxy-terminal third of a(II)PPSS was processed and secreted via a regulated pathway. We conclude that the amino-terminal 78 residues of rPPSS contain sufficient information to correct the targeting deficiency of a(II)PPSS in mammalian endocrine cell lines. 相似文献