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151.
152.
Opportunities of marker‐assisted selection for rice fragrance through marker–trait association analysis of microsatellites and gene‐based markers
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F. S. Golestan Hashemi M. Y. Rafii M. Razi Ismail M. T. M. Mohamed H. A. Rahim M. A. Latif F. Aslani 《Plant biology (Stuttgart, Germany)》2015,17(5):953-961
Developing fragrant rice through marker‐assisted/aided selection (MAS) is an economical and profitable approach worldwide for the enrichment of an elite genetic background with a pleasant aroma. The PCR‐based DNA markers that distinguish the alleles of major fragrance genes in rice have been synthesised to develop rice scent biofortification through MAS. Thus, the present study examined the aroma biofortification potential of these co‐dominant markers in a germplasm panel of 189 F2 progeny developed from crosses between a non‐aromatic variety (MR84) and a highly aromatic but low‐yielding variety (MRQ74) to determine the most influential diagnostic markers for fragrance biofortification. The SSRs and functional DNA markers RM5633 (on chromosome 4), RM515, RM223, L06, NKSbad2, FMbadh2‐E7, BADEX7‐5, Aro7 and SCU015RM (on chromosome 8) were highly associated with the 2AP (2‐acetyl‐1‐pyrroline) content across the population. The alleles traced via these markers were also in high linkage disequilibrium (R2 > 0.70) and explained approximately 12.1, 27.05, 27.05, 27.05, 25.42, 25.42, 20.53, 20.43 and 20.18% of the total phenotypic variation observed for these biomarkers, respectively. F2 plants harbouring the favourable alleles of these effective markers produced higher levels of fragrance. Hence, these rice plants can be used as donor parents to increase the development of fragrance‐biofortified tropical rice varieties adapted to growing conditions and consumer preferences, thus contributing to the global rice market. 相似文献
153.
An electrochemical impedance spectroscopy (EIS) assay measuring the calcification inhibition capacity in biological fluids 总被引:1,自引:0,他引:1
Ismail AH Schäfer C Heiss A Walter M Jahnen-Dechent W Leonhardt S 《Biosensors & bioelectronics》2011,26(12):4702-4707
Pathological calcification of the cardiovascular system is one of the major causes of high mortality and morbidity in dialysis patients. The inhibition of ectopic calcification relies (I) on the formation of calciprotein particles (CPPs), nanospherical complexes of calcium phosphate mineral, fetuin-A and other acidic serum proteins, and (II) on the stabilization of calcium phosphate prenucleation clusters by fetuin-A monomers. In supersaturated serum, mineral ion aggregation leads to a change in the electrical impedance. In this work, we present a method based on electrochemical impedance spectroscopy (EIS) to establish an impedance trace of mineral ion clustering in vitro. In the presence of 20 μM of serum protein fetuin-A, a prototypic calcification inhibitor, we measured a change in impedance (Δ(R)) of 195.52 ± 27.78%Ω compared to 430.41 ± 11.36%Ω in inhibitor-free samples. We also identified a CPP-formation dependency on the actual content of ions and protein in the samples under investigation. Two-step ripening of CPP was also observed. The presented method may form the basis of a simple label-free bedside or online test to be used in routine clinical practice for estimating the calcification risk in serum. 相似文献
154.
Ali MA Ismail R Choon TS Pandian S Hassan Ansari MZ 《Journal of enzyme inhibition and medicinal chemistry》2011,26(4):598-602
In this study, a series of novel 3-(substituted phenyl)-6,7-dimethoxy-3a,4-dihydro-3H-indeno[1,2-c]isoxazole analogues were synthesized and evaluated for antimycobacterial activity against Mycobacterium tuberculosis (MTB) H(37)Rv and isoniazid resistant M. tuberculosis (INHR-MTB). All the newly synthesized compounds were showing moderate to high inhibitory activities. The compound 6,7-dimethoxy-3-(4-chloro phenyl)-4H-indeno[1,2-c]isoxazole (4b) was found to be the most promising compound, active against MTB H(37)Rv and INHR-MTB with minimum inhibitory concentrations of 0.22 and 0.34 μM. 相似文献
155.
Abdel-Rahman MA Abdel-Nabi IM El-Naggar MS Abbas OA Strong PN 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2011,154(4):318-325
A combination of proteomic and biochemical assays was used to examine variations in the venom of Conus vexillum taken from two locations (Hurgada and Sharm El-Shaikh) in the Red Sea, Egypt. Using MALDI/TOF-MS, a remarkable degree of intra-species variation between venom samples from both locations was identified. To evaluate variability in the cytotoxic effects of Conus venom, mice were injected with the same dose from each location. The oxidative stress biomarkers [malondialdehyde (MDA), protein carbonyl content (PCC)], antioxidants [glutathione (GSH), superoxide dismutase (SOD), catalase (CAT)], total antioxidant capacity (TAC) and nitric oxide (NO), were measured 3, 6, 9 and 12 h post venom injection. The venoms induced a significant increase in the levels of PCC, MDA, NO, GSH and CAT. The venoms significantly inhibited the activity of SOD and reduced the TAC. Toxicological data showed that the venom obtained from Hurgada was more potent than that obtained from Sharm El-Shaikh. It can be concluded that: (1) the venom of the same Conus species from different regions is highly diversified (2) the venoms from different locations reflect clear differences in venom potency and (3) the cytotoxic effects of C. vexillum venom can be attributed to its ability to induce oxidative stress. 相似文献
156.
Habib Hamidinezhad Yussof Wahab Zulkafli Othaman Abd Khamim Ismail 《Plasmonics (Norwell, Mass.)》2011,6(4):791-796
Silicon nanonowires (SiNWs) were synthesized with approximately 100 nm/min by very-high-frequency plasma-enhanced chemical
vapor deposition method via a vapor–liquid–solid mechanism. Pure silane gas was deposited on Au-coated silicon substrates
at various radio frequency (RF) powers varied from 5 to 20 W, keeping other deposition parameters constant. Synthesized SiNWs
were needle-like shape with an ultra-sharp tip diameter of about 10 nm and a length about 3 μm, which differs from the standard
cylindrical nanowire produced by similar techniques. Furthermore, it was shown that the average length of nanoneedles augmented
with the increase of RF power. High-resolution transition electron microscopy displayed that the nanowires were composed of
a crystalline Si core with an amorphous oxide shell. Raman spectroscopy also revealed the presence of crystalline Si in the
grown Si nanoneedles. 相似文献
157.
This study aimed to express two major drug-metabolizing human hepatic cytochromes P450 (CYPs), CYP2D6 and CYP3A4, together
with NADPH-cytochrome P450 oxidoreductase (OxR) in Escherichia coli and to evaluate their catalytic activities. Full length cDNA clones of both isoforms in which the N-terminus was modified
to incorporate bovine CYP17α sequence were inserted into a pCWori+ vector. The modified CYP cDNAs were subsequently expressed individually, each together with OxR by means of separate, compatible
plasmids with different antibiotic selection markers. The expressed proteins were evaluated by immunoblotting and reduced
CO difference spectral scanning. Enzyme activities were examined using high performance liquid chromatography (HPLC) assays
with probe substrates dextromethorphan and testosterone for CYP2D6 and CYP3A4, respectively. Results from immunoblotting demonstrated
the presence of both CYP proteins in bacterial membranes and reduced CO difference spectra of the cell preparations exhibited
the characteristic absorbance peak at 450 nm. Co-expressed OxR also demonstrated an activity level comparable to literature
values. Kinetic parameters, Km and Vmax values determined from the HPLC assays also agreed well with literature values. As a conclusion, the procedures described
in this study provide a relatively convenient and reliable means of producing catalytically active CYP isoforms suitable for
drug metabolism and interaction studies. 相似文献
158.
目的:总结腰椎间盘突出症的临床特点及诊治要点。方法:回顾性分析260例腰椎间盘突出症手术患者的临床资料。结果:直腿抬高与影像学检查结果符合率为100%,治疗优良率达88.08%,有效率100%。结论:腰、下肢和臀部疼痛、下肢麻木、体位改变、运动障碍、感觉障碍、肌萎缩都是腰椎间盘突出症的主要临床表现;直腿抬高试验高试验可作为早期诊断的重要参考指标,要要根据惠者体征、病程等具体情况选择适合的最佳治法。 相似文献
159.
Bahaa T. Shawky Manal G. Mahmoud Eman A. Ghazy Mohsen M.S. Asker Gada S. Ibrahim 《Journal of Genetic Engineering and Biotechnology》2011,9(1):59-63
Rice straw and corn stalks were used as fermentation substrate for the evaluation of cellulases activity secreted by different organisms. The substrates were pretreated with alkaline hydrogen peroxide (AHP) for 6 h at 30 and 60 °C. From the fermentation studies, rice straw and corn stalks substrates showed the highest cellulases activity after 96 h at 60 °C of pre-treatment. 相似文献
160.