全文获取类型
收费全文 | 8204篇 |
免费 | 894篇 |
国内免费 | 41篇 |
专业分类
9139篇 |
出版年
2022年 | 71篇 |
2021年 | 140篇 |
2020年 | 80篇 |
2019年 | 89篇 |
2018年 | 104篇 |
2017年 | 103篇 |
2016年 | 179篇 |
2015年 | 357篇 |
2014年 | 356篇 |
2013年 | 435篇 |
2012年 | 576篇 |
2011年 | 582篇 |
2010年 | 328篇 |
2009年 | 274篇 |
2008年 | 426篇 |
2007年 | 377篇 |
2006年 | 368篇 |
2005年 | 355篇 |
2004年 | 340篇 |
2003年 | 281篇 |
2002年 | 275篇 |
2001年 | 222篇 |
2000年 | 246篇 |
1999年 | 216篇 |
1998年 | 100篇 |
1997年 | 80篇 |
1996年 | 69篇 |
1995年 | 68篇 |
1994年 | 60篇 |
1993年 | 75篇 |
1992年 | 150篇 |
1991年 | 126篇 |
1990年 | 139篇 |
1989年 | 127篇 |
1988年 | 140篇 |
1987年 | 108篇 |
1986年 | 106篇 |
1985年 | 115篇 |
1984年 | 84篇 |
1983年 | 46篇 |
1982年 | 45篇 |
1981年 | 49篇 |
1980年 | 47篇 |
1979年 | 78篇 |
1978年 | 73篇 |
1977年 | 61篇 |
1976年 | 48篇 |
1974年 | 47篇 |
1973年 | 51篇 |
1972年 | 39篇 |
排序方式: 共有9139条查询结果,搜索用时 11 毫秒
31.
32.
33.
DETERMINATION BY NEUTRON ACTIVATION OF COPPER, MANGANESE, AND ZINC IN THE PINEAL BODY AND OTHER AREAS OF BRAIN TISSUE 总被引:2,自引:1,他引:1
Abstract— Neutron activation analysis was used for the simultaneous determination of copper, manganese and zinc in the brains of calves, cows and pigs. Measurements of these three elements in as many as 11 different regions of the brain showed that the highest content is always found in the pineal body. Typical values for calf brains are: Cu, ∼20 μg/g; Mn, ∼3 μg/g; and Zn, ∼90 μg/g dry pineal tissue. As a first approximation, the ratio of Cu:Mn:Zn is roughly constant for all regions. 相似文献
34.
35.
F Hérve M Gentin K M Rajkowski L T Wong C J Hsia N Cittanova 《Journal of steroid biochemistry》1990,36(4):319-324
Rat fetal serum alpha 1-fetoprotein (AFP), a heterogeneous glycoprotein, binds estrogens with high affinity but at a fractional number of sites even after treatment with charcoal (n = 0.6), which may mean 60% of the protein has 1 site and the remainder none. To investigate the origin of this fractional number of sites the "native" protein (purified by negative affinity chromatography) was further purified (step 1) and fractionated (step 2) into its two main charge variants (electrophoretically "slow" and "fast") by a two-step fast-protein liquid chromatography method. The binding parameters for estrone and estradiol-17 beta of the "native" and "repurified" proteins and of each charge variant were determined by equilibrium microdialysis. The molar extinction coefficient at 278 nm of each sample was also determined. (1) The "repurified" AFP and each charge variant had a number of binding sites for estrogens close to unity. This increase in the number of sites could neither be explained by the loss of a non-binding isoform (corresponding to 40% of the protein) during chromatography, nor by the existence of complex negative modulatory interactions between isoforms. (2) The affinities for estrogens of the "repurified" protein and the two charge variants were slightly decreased compared to that of "native" AFP, except that the "fast" form had the "native" protein's high affinity for estrone--but not for estradiol-17 beta. (3) The molar extinction coefficients at 278 nm of the "repurified" AFP and the isoforms were much lower than that of the "native" protein. These results suggest that the presence of (an) inhibitor(s) of estrogen binding on the "native" protein which is/are removed by the ion-exchange fast protein liquid chromatography (FPLC) column. A ligand absorbing at 278 nm, which may or may not be the inhibitor, is also removed. The isoform heterogeneity with respect to estrone binding is discussed. 相似文献
36.
Previous studies have shown that peripheral blood mononuclear cells activated in vitro not only express cell-associated interleukin 2 receptors (IL2R) but also release a soluble form of this receptor. In this study, we demonstrate that administration of human recombinant IL 2 (rIL 2) to mice results in increased spleen weights, splenic natural killer (NK) cell cytolytic activity, and serum levels of soluble IL2R. However, compared with rIL 2-treated heterozygote controls, beige mice treated with rIL 2 displayed similar elevations in serum soluble IL2R but significantly less splenic NK activity. Likewise, administration of anti-asialo GM1 antiserum to rIL 2-treated mice resulted in a dramatic reduction in splenic NK cytolytic activity, but no reduction in serum soluble IL2R. Conversely, while rIL 2 treatment of BALB/c mice produced increased splenic NK activity and serum soluble IL2R, similar treatment of BALB/c nude mice resulted in elevation of only splenic NK activity. These studies demonstrate that administration of rIL 2 to normal mice can elevate both serum IL2R levels and splenic NK cytolytic activity. However, the results suggest that T cells are likely to be the source of elevated serum IL2R after rIL 2 administration. 相似文献
37.
Low multiplicity infection of insect cells with a recombinant baculovirus: The cell yield concept 总被引:3,自引:0,他引:3
Wong KT Peter CH Greenfield PF Reid S Nielsen LK 《Biotechnology and bioengineering》1996,49(6):659-666
In vitro infection of insect cells with baculoviruses is increasingly considered a viable means for the production of biopesticides, recombinant veterinary vaccines, and other recombinant products. Batch fermentation processes traditionally employ intermediate to high multiplicities of infection necessitating two parallel scale-up processes-one for cells and one for virus. In this study, we consider the use of multiplicities of infection as low as 0.0001 plaque-forming units per cell, a virus level low enough to enable infection of even large reactors (e.g., 10 m(3)) directly from a frozen stock. Using low multiplicities in the Sf9/beta-gal-AcNPV system, recombinant protein titers comparable with the maximum titer observed in high multiplicity infections were achieved. Cultures yielding the maximum titer were characterized by reaching a maximum cell density between 3 and 4 x 10(9) cell L(-1). This optimal cell yield did not depend on the multiplicity of infection, supporting the existing view that batch cultures are limited by availability of substrate. Up to a certain cell density, product titer will increase almost linearly with availability of biocatalyst, that is, cells. Beyond this point any further cell formation comes at the expense of final product titer. Low multiplicity infections were found not to cause any significant dispersion of the protein production process. Hence, product stability is not a major issue of concern using low multiplicities of infection. The sensitivity to initial conditions and disturbances, however, remains an issue of concern for the commercial use of low multiplicity infections. (c) 1996 John Wiley & Sons, Inc. 相似文献
38.
The aim of the present study was to separate and characterise products formed by oxidation of uric acid by hydroxyl radicals with a view to probing for these products in vivo in clinical contexts. Aerated solutions of 200 μM uric acid, or its oxidation products, allantoin or parabanic acid, were exposed to gamma radiolysis, (52.0 Gy/min), as a source of HO- radicals, at pH 3.4 and 7.4. Aliquots were taken every 5 minutes for 20 minutes and oxidation products were separated by HPLC and analysed with a diode array detector. Identities of oxidation products were confirmed on the basis of similarity of retention times and absorbance spectra and peak purity parameters of known standards. Hydroperoxides were measured by tri-iodide formation in the 20 minute sample. Exposure of uric acid to such HO fluxes produced a net loss of the parent compound with formation of a complex mixture of products with allantoin and parabanic acid being the predominant products at pH 3.4. The rate of uric acid degradation at physiological pH was slower and the distribution of oxidation products was different. A small but significant amount of uric acid hydroperoxide was detected at both pHs. A mechanism for uric acid oxidation under these conditions is presented. 相似文献
39.
Neuza Domingues André R. A. Marques Rita Diogo Almeida Calado Inês S. Ferreira Cristiano Ramos José Ramalho Maria I. L. Soares Telmo Pereira Luís Oliveira José R. Vicente Louise H. Wong Inês C. M. Simões Teresa M. V. D. Pinho e Melo Andrew Peden Cláudia Guimas Almeida Clare E. Futter Rosa Puertollano Winchil L. C. Vaz Otília V. Vieira 《Traffic (Copenhagen, Denmark)》2023,24(7):284-307
40.