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41.
Mamoru Tsukuda Izumi Mochimatsu Miki Sakumoto Yasukazu Mikami Seiichiro Yuyama Shunsuke Yanoma 《Biotherapy》1993,6(3):167-174
Interleukin-7 (IL-7) has an ability to stimulate the proliferation of pre-B cells. It has been shown that IL-7 can also activate T lymphocytes. We here demonstrate that IL-7 in combination with interleukin-2 (IL-2) can drive cell proliferation and enhance the autologous tumor cell lysis by peripheral blood mononuclear cells (PBMC) and autologous mixed lymphocyte tumor cell culture (MLTC)-derived effector cells (MLTC cells). These synergistic effects of IL-2 and IL-7 on the proliferation and the augmentation of autologous tumor cell lysis were found for both effector cells. These effects were inhibited by neutralizing antibodies to IL-2 or IL-7, and by a combination of both antibodies, significantly. In terms of phenotypical expression, CD3 positive cells comprised the vast majority of MLTC cells after culture in medium containing IL-2 and IL-7 with an increase of IL-2 receptor positive cells.Abbreviations CD
cluster differentiation
- IFN
interferon
- IL
interleukin
- JRU
Japanese Reference Unit
- LAK
lymphokine activated killer
- mAb
monoclonal antibody
- MLTC
mixed lymphocyte tumor cell culture
- PBMC
peripheral blood mononuclear cells
- TILs
tumor infiltrating lymphocytes 相似文献
42.
43.
44.
Tadashi II Masayuki Kubota Takashi Hirano Mamoru Ohashi Keiichi Yoshida Sakaru Suzuki 《Glycoconjugate journal》1995,12(3):282-289
The fast atom bombardment (FAB) collision induced dissociation (CID)-mass spectrometry/mass spectrometry (MS/MS) technique was successfully applied to characterize and identify the structures of the immunoreactive trisulfated and tetrasulfated tetrasaccharides that were obtained from the chondroitin sulfate in a shark fin using a treatment with chondroitinase ABC.Abbreviations FABMS
fast atom bombardment mass spectrometry
- CID
collision induced dissociation
- MS/MS
mass spectrometry/mass spectrometry
- UA2S-GalNAc6S
2-acetamido-2-deoxy-3-O-(2-O-sulfo--d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-galactose
- UA-GalNAc4S
2-acetamido-2-deoxy-3-O-(-d-gluco-4-enepyranosyluronic acid)-4-O-sulfo-d-galactose
- UA-GalNAcDiS
2-acetamido-2-deoxy-3-O-(-d-gluco-4-enepyranosyluronic acid)-4,6-di-O-sulfo-d-galactose 相似文献
45.
Hideyuki Takahashi Mitsuharu Inaki Koichi Fujimoto Shigeru Katsuta Izumi Anno Mamoru Nütsu Yuji Itai 《European journal of applied physiology and occupational physiology》1995,71(5):396-404
We examined the effect of differences in exercise intensity on the time constant (t
c) of phosphocreatine (PCr) resynthesis after exercise and the relationships betweent
c and maximal oxygen uptake (VO2max) in endurance-trained runners (n = 5) and untrained controls (n = 7) (average VO2max = 66.2 and 52.0 ml · min–1 · kg–1, respectively). To measure the metabolism of the quadriceps muscle using phosphorus nuclear magnetic resonance spectroscopy, we developed a device which allowed knee extension exercise inside a magnet. All the subjects performed four types of exercise: light, moderate, severe and exhausting. The end-exercise PCr: [PCr + inorganic phosphate (Pi)] ratio decreased significantly with the increase in the exercise intensity (P < 0.01). Although there was little difference in the end-exercise pH, adenosine diphosphate concentration ([ADP]) and the lowest intracellular pH during recovery between light and moderate exercise, significant changes were found at the two higher intensities (P < 0.01). These changes for runners were smaller than those for the controls (P < 0.05). The
c remained constant after light and moderate exercise and then lengthened in proportion to the increase in intensity (P < 0.05). The runners had a lowert
c at the same PCr and pH than the controls, particularly at the higher intensity (P < 0.05). There was a significant correlation betweent
c and [ADP] in light exercise and betweent
c and both end-exercise PCr and pH in severe and exhausting exercise (P < 0.05). The threshold of changes in pH andt
c was a PCr: (PCr + Pi) ratio of 0.5. There was a significant negative correlation between the VO2max andt
c after all levels of exercise (P<0.05).However, in the controls a significant correlation was found in only light and moderate exercise (P < 0.05). These findings suggest the validity of the use oft
c at an end-exercise PCr:(PCr + Pi) ratio of more than 0.5 as a stable index of muscle oxidative capacity and the correlation between local and general aerobic capacity. Moreover, endurance-trained runners are characterized by the faster PCr resynthesis at the same PCr and intracellular pH. 相似文献
46.
Hideki Iwata Shunji Tomatsu Seiji Fukuda Atsushi Uchiyama G. M. M. Rezvi Tatsuya Ogawa Toshinori Hori Yoshihiro Nakashima Atsushi Yamagishi Kazuko Sukegawa Nobuyuki Shimozawa Yasuyuki Suzuki Naomi Kondo Tadao Orii 《Human genetics》1995,95(3):257-264
Seven different restriction fragment length polymorphisms (RFLPs) at the N-acetylgalactosamine-6-sulfate sulfatase (GALNS) locus were analyzed using Southern blotting and polymerase chain reaction based techniques to search for the frequency of each RFLP produced by StyI, SphI, HaeIII, StuI, HapII, XhoI, and BamHI restriction endonucleases, respectively, in 36 mutant alleles, including two sibling cases and 100 normal alleles. Calculation of heterozygosity indexes showed that these RFLPs were polymorphic, ranging from 0.31 to 0.69 in mucopolysaccharidosis IVA (MPS IVA) patients compared with 0.21 to 0.65 in normal individuals. There was some significant difference in several RFLPs and in the combination with four kinds of RFLPs (SphI, StuI, HapII, XhoI polymorphisms). The normal alleles were composed of 13 different RFLPs haplotypes; the most common among the Japanese population carrying normal alleles was haplotype 8 (bDEF1) (31.3%), the others being dispersed. The same haplotype 8 was the most frequent in the mutant alleles (44.4%), with seven further haplotypes. These findings revealed the striking variety of polymorphic haplotypes in the MPS IVA gene. By using these five kinds of RFLPs, we examined the theoretical informativity of haplotype analysis in heterozygote detection in nine unrelated MPS IVA families and ten unrelated normal families. All the members of the MPS IVA families studied were diagnosed as a patient, carrier, or noncarrier. We propose that prenatal diagnosis or family analysis in cases in which mutations have not been characterized is now feasible. 相似文献
47.
Low-humidity treatment triggered an increase of the thallus area and the chlorophyll content in the following high-humidity
condition. When low-humidity treatment for 4 days was intercalated in high-humidity conditions every 16 days, the thallus
area increased 40% in 76 days. 相似文献
48.
Kazuyosh Iwata 《Journal of plant research》1995,108(4):531-534
The orientation of cortical microtubules (MTs) was synchronously regulated inSpirogyra cells. While the reorganized MTs in distilled water for 1.5 hr, after 1 hr treatment with amiprophos-methyl (APM) and complete
depolymerization of the MTs, were all transverse, those reorganized in 0.30 M mannitol were all oblique or longitudinal. After
the MTs had reorganized in 0.30 M mannitol, these cells were then incubated in distilled water for 6 hr, and the orientation
of the MTs, in the cells in which MTs could be observed, all became transverse. 相似文献
49.
Shinji Hosoi Mitsuo Satoh Hiromasa Miyaji Tatsunari Nishi Tamio Mizukami Mamoru Hasegawa Seiga Itoh Tatsuya tamaoki 《Cytotechnology》1995,19(1):1-10
Pro-UKS1 was designed as a thrombin-resistant derivative of pro-urokinase (pro-UK) by introducing a glycosylation site using site-directed mutagenesis. An expression plasmid for pro-UKS1, pMo1UKS1SEd1-5, was constructed and introduced into Namalwa KJM-1 cells (Hosoiet al., 1988), and cells resistant to G418 and Methotrexate (MTX) were obtained. Amongst them, the highest pro-UKS1 producer (resistant to 500 nM of MTX), clone 41-8, was selected and further characterized. Clone 41-8 was cultured in serum-free ITPSGF medium (Hosoiet al., 1988). Under the conventional conditions, the concentration of pro-UKS1 reached 26 g ml–1. Addition of glucose and tri-iodothyronine (T3) improved productivity, and the maximal productivity of pro-UKS1 was 67 g ml–1 day–1. In this conditioned medium, content of pro-UKS1 was above 80% of total proteins.Abbreviations BSA
bovine serum albumin
- dhfr
dihydrofolate reductase
- HEPES
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
- kb
kilobase pairs
- kDa
kilodaltons
- MTX
Methotrexate
- PBS
phosphate buffered saline
- pro-UK
pro-urokinase
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- T3
tri-iodothyronine
- Tween-PBS
phosphate buffered saline containing 0.05% Tween 80 相似文献
50.
The growth of lichens in the field is slow and their cultivation is generally thought to be difficult. We studied the effects
of environmental conditions and culture solutions on the growth of a lichen, and found that growth ofParmotrema tinctorum (Nyl.) Hale in growth cabinets was possible. The thallus area increased by about 20% monthly when the lichen was soaked in
a culture solution for 90 min every four days and then grown at 100% relative humidity when the temperature in the growth
cabinet was 20C and illumination was at 12 W/m2 for 16 hr daily. 相似文献