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51.
52.
Aleksey Y. Malyshev Pavel M. Balaban 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2011,197(1):25-32
Bilaterally symmetrical pair of serotonergic cells, named C1 in Clione, has been described in the cerebral ganglia of all gastropod species. Here we describe a new role of C1 cells in gastropod
mollusks: control of activity of ciliated epithelium in the foregut. Detailed morphological investigation of C1 neurons in
the pteropod mollusk Clione
limacina revealed that these cells among other destinations send their neurites into foregut where they produce intense arborization
with large varicosities along the processes. Intracellular stimulation of a single C1 induced pronounced activation (often
followed by inhibition) of cilia lining the foregut. This activation was substantially reduced by serotonin antagonist mianserin.
Bath application of serotonin also induced transient increase in ciliary transport rate, followed by inhibition of ciliary
activity up to its full cessation in some areas of isolated foregut. These data suggest that C1 in Clione may use serotonin to influence cilia in the foregut. Taking into account high homology of serotonergic cerebral cells across
studied species we can speculate that these cells may be involved in the neural control of cilia in the foregut in other gastropod
mollusks. 相似文献
53.
S. J. Malyshev 《Zoomorphology》1930,19(1):116-134
Ohne Zusammenfassung 相似文献
54.
S. J. Malyshev 《Zoomorphology》1930,16(3-4):541-558
Ohne Zusammenfassung 相似文献
55.
S. J. Malyshev 《Zoomorphology》1928,11(5):763-781
Ohne Zusammenfassung
Mit 7 Textabbildungen 相似文献
56.
Zhirnov OP Vorobjeva IV Saphonova OA Malyshev NA Ovcharenko AV Klenk HD 《Biochemistry. Biokhimii?a》2007,72(4):398-408
Influenza A viruses isolated from the respiratory tract of patients with influenza were cultured in human intestinal epithelium cells (CACO-2 line). The CACO-2 cells were found to be 100-fold more susceptible to the clinical viruses than MDCK cells and chicken embryos. On passaging in CACO-2 cells, clinical isolates of the subtype H3N2 retained the original "human" phenotype and agglutinated human but not chicken erythrocytes, whereas on passaging in MDCK cells the virus phenotype changed to the "avian" one. On comparison with laboratory strains (grown in chicken embryos or MDCK cells), the clinical viruses were characterized by higher stability of the anti-interferon protein NS1 but had a reduced synthesis of the matrix protein M1, and this could facilitate the virus adaptation and escape of the infected cells from immune attack in the human body. The increased tropism to the human CACO-2 cells correlated with higher adsorption of the clinical viruses on cellular receptors. However, in the CACO-2 and MDCK cells the ratio of sialyl-containing glycoreceptors of the 2-3 and 2-6 type was similar. These observations indicated that not only sialic acid residues were involved in the adsorption and penetration of the clinical viruses into human cells, but also the protein moiety of the cellular receptor itself and/or an additional cellular coreceptor. Thus, clinical influenza viruses are shown to possess a specific mechanism of sorption and entry into human epithelial cells, which is responsible for their higher tropism to human cells and is unlike such a mechanism in canine cells. 相似文献
57.
58.
The results of studying the color variation of the red wood ant F. aquilonia in Northwestern Russia and Fennoscandia are presented. Pronounced phenotypic differentiation among the ant populations was observed, which may be interpreted in the light of postglacial dispersal of the species. Ants from Karelia and the environs of Moscow showed high similarity in their color variation. The share of the light morph Pn3 increased in more northern localities, namely the Pasvik Reserve, and also in more western ones, namely St. Petersburg and Espoo. Thus, there were two vectors of ant dispersal: the northern and the western ones, coinciding with the known routes of postglacial recolonization by small mammals. Analysis of color variation is a very promising approach to the study of intraspecific structure and ways of its formation. 相似文献
59.
60.
Pandhal J Desai P Walpole C Doroudi L Malyshev D Wright PC 《Biochemical and biophysical research communications》2012,419(3):472-476
Recently, efforts to increase the toolkit which Escherichia coli cells possess for recombinant protein production in industrial applications, has led to steady progress towards making glycosylated therapeutic proteins. Although the desire to make therapeutically relevant complex proteins with elaborate human-type glycans is a major goal, the relatively poor efficiency of the N-glycosylation process of foreign proteins in E. coli remains a hindrance for industry take-up. In this study, a systematic approach was used to increase glycoprotein production titres of an exemplar protein, AcrA, and the resulting glycosylation efficiency was quantified using a combination of Western blots and pseudo Selective Reaction Monitoring (pSRM). Western blot and pSRM results demonstrate that codon optimising the oligosaccharyltransferase, PglB, for E. coli expression, increases efficiency by 77% and 101%, respectively. Furthermore, increasing expression of glycosyltransferase, WecA, in E. coli improves efficiency by 43% and 27%, respectively. However, increasing the amount of donor lipid used in the glycosylation process did not impact on the glycosylation efficiency in this system, with this specific protein. 相似文献