全文获取类型
收费全文 | 416篇 |
免费 | 26篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 9篇 |
2020年 | 5篇 |
2019年 | 6篇 |
2018年 | 10篇 |
2017年 | 4篇 |
2016年 | 16篇 |
2015年 | 23篇 |
2014年 | 29篇 |
2013年 | 27篇 |
2012年 | 47篇 |
2011年 | 32篇 |
2010年 | 17篇 |
2009年 | 21篇 |
2008年 | 21篇 |
2007年 | 26篇 |
2006年 | 30篇 |
2005年 | 22篇 |
2004年 | 17篇 |
2003年 | 17篇 |
2002年 | 11篇 |
2001年 | 2篇 |
1999年 | 4篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1988年 | 2篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1978年 | 2篇 |
1976年 | 2篇 |
1964年 | 1篇 |
排序方式: 共有442条查询结果,搜索用时 15 毫秒
101.
102.
103.
104.
Traditional theories of texture segregation suggest that elementary visual features are processed in parallel by independent modules at early visual stages. Here we show that, for small feature contrasts and large values evoking perceptual popout, different forms of module interaction exist. While discrimination of highly salient features rests on independent feature specific pathways, information is summed across domains when barely noticeable ones are to be detected in homogeneous textures. 相似文献
105.
Nalaskowski MM Bertsch U Fanick W Stockebrand MC Schmale H Mayr GW 《The Journal of biological chemistry》2003,278(22):19765-19776
The calcium-liberating second messenger inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) is converted to inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4) by Ins(1,4,5)P3 3-kinases (IP3Ks) that add a fourth phosphate group to the 3-position of the inositol ring. Two isoforms of IP3Ks (named A and B) from different vertebrate species have been well studied. Recently the cloning and examination of a human full-length cDNA encoding a novel isoform, termed human IP3K-C (HsIP3K-C), has been reported. In the present study we report the cloning of a full-length cDNA encoding a rat homologue of HsIP3K-C with a unique mRNA expression pattern, which differs remarkably from the tissue distribution of HsIP3K-C. Of the rat tissues examined, rat IP3K-C (RnIP3K-C) is mainly present in heart, brain, and testis and shows the strongest expression in an epidermal tissue, namely tongue epithelium. RnIP3K-C has a calculated molecular mass of approximately 74.5 kDa and shows an overall identity of approximately 75% with HsIP3K-C. A bacterially expressed, enzymatically active and Ca2+-calmodulin-regulated fragment of this isoform displays remarkable enzymatic properties like a very low Km for Ins(1,4,5)P3 ( approximately 0.2 microm), substrate inhibition by high concentrations of Ins(1,4,5)P3, allosteric product activation by Ins(1,3,4,5)P4 in absence of Ca2+-calmodulin (Ka(app) 0.52 microm), and the ability to efficiently phosphorylate a second InsP3 substrate, inositol 2,4,5-trisphosphate, to inositol 2,4,5,6-tetrakisphosphate in the presence of Ins(1,3,4,5)P4. Furthermore, the RnIP3K-C fused with a fluorescent protein tag is actively transported into and out of the nucleus when transiently expressed in mammalian cells. A leucine-rich nuclear export signal and an uncharacterized nuclear import activity are localized in the N-terminal domain of the protein and determine its nucleocytoplasmic shuttling. These findings point to a particular role of RnIP3K-C in nuclear inositol trisphosphate phosphorylation and cellular growth. 相似文献
106.
Rassaf T Bryan NS Maloney RE Specian V Kelm M Kalyanaraman B Rodriguez J Feelisch M 《Nature medicine》2003,9(5):481-2; author reply 482-3
107.
108.
109.
Kaya Z Dohmen KM Wang Y Schlichting J Afanasyeva M Leuschner F Rose NR 《Journal of immunology (Baltimore, Md. : 1950)》2002,168(4):1552-1556
Appropriate treatment of autoimmune myocarditis following virus infection remains a major clinical problem. Induction of nasal tolerance may provide a new approach to treatment. However, the exact mechanism of nasal tolerance is unknown. To assess the mechanism of nasal tolerance, we examined the role of IL-10 in the induction and suppression of autoimmune myocarditis. First we showed that blocking IL-10 concurrent with nasal administration of Ag abolished the disease-suppressing effect of nasal tolerization. It also led to increased cardiac myosin-specific IL-1 and TNF-alpha production. Then we demonstrated that blocking IL-10 during the effector phase increased not only the incidence and severity of disease but also Ag-specific IL-2, IL-4, and TNF-alpha production as well as cardiac myosin-specific IgG1 and IgG2b production, whereas blocking IL-10 during the induction phase had no effect. This study implicates IL-10 in the induction of nasal tolerance and in limiting inflammation later during the disease process. 相似文献
110.
Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells 总被引:1,自引:0,他引:1
Gruss OJ Wittmann M Yokoyama H Pepperkok R Kufer T Silljé H Karsenti E Mattaj IW Vernos I 《Nature cell biology》2002,4(11):871-879
In Xenopus laevis egg extracts, TPX2 is required for the Ran-GTP-dependent assembly of microtubules around chromosomes. Here we show that interfering with the function of the human homologue of TPX2 in HeLa cells causes defects in microtubule organization during mitosis. Suppressing the expression of human TPX2 by RNA interference leads to the formation of two microtubule asters that do not interact and do not form a spindle. Our results suggest that in vivo, even in the presence of duplicated centrosomes, spindle formation requires the function of TPX2 to generate a stable bipolar spindle with overlapping antiparallel microtubule arrays. This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells. 相似文献