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551.
Göran M. Nylund Frank Persson Mats Lindegarth Gunnar Cervin Malte Hermansson & Henrik Pavia 《FEMS microbiology ecology》2010,71(1):84-93
Ecological research on algal-derived metabolites with antimicrobial activity has recently received increased attention and is no longer only aimed at identifying novel natural compounds with potential use in applied perspectives. Despite this progress, few studies have so far demonstrated ecologically relevant antimicrobial roles of algal metabolites, and even fewer have utilized molecular tools to investigate the effects of these metabolites on the natural community composition of bacteria. In this study, we investigated whether the red alga Bonnemaisonia asparagoides is chemically defended against bacterial colonization of its surface by extracting surface-associated secondary metabolites and testing their antibacterial effects. Furthermore, we compared the associated bacterial abundance and community composition between B. asparagoides and two coexisting macroalgae. Surface extracts tested at natural concentrations had broad-spectrum effects on the growth of ecologically relevant bacteria, and consistent with this antibacterial activity, natural populations of B. asparagoides had significantly lower densities of epibacteria compared with the coexisting algae. Terminal restriction fragment length polymorphism analysis further showed that B. asparagoides harboured surface-associated bacteria with a community composition that was significantly different from those on coexisting macroalgae. Altogether, these findings demonstrate that B. asparagoides produces surface-bound antibacterial compounds with a significant impact on the abundance and composition of the associated bacterial community. 相似文献
552.
Allotetraploid hybrids between citrus and seven related genera produced by somatic hybridization 总被引:2,自引:0,他引:2
J. W. Grosser F. A. A. Mourao-Fo F. G. Gmitter Jr. E. S. Louzada J. Jiang K. Baergen A. Quiros C. Cabasson J. L. Schell J. L. Chandler 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(5):577-582
We have developed an efficient protoplast-fusion method to produce somatic hybrid allopolyploid plants that combine Citrus with seven related genera, including four that are sexually incompatible. In this paper we report the creation of 18 new allotetraploid hybrids of Citrus, including ten among sexually incompatible related genera, that may have direct cultivar potential as improved citrus rootstocks. All hybrids were confirmed by cytological and RAPD analyses. If fertile, the attributes of these hybrids may be amenable to further genetic manipulation by breeding at the tetraploid level. Wide somatic hybridization of Citrus via protoplast fusion bypasses biological barriers to the natural allopolyploidization of Citrus, and creates new evolutionary opportunities that would be difficult or impossible to achieve by natural or conventional hybridization.Florida Agricultural Experiment Station Journal Series No. R-04520 相似文献