子痫前期患者血清APN、TNF-α、Apelin水平的表达及相关性研究

目的:探讨子痫前期患者血清脂联素(APN)、肿瘤坏死因子-α(TNF-α)、Apelin水平的表达及相关性。方法:选取2015年10月到2017年8月在辽河油田总医院妇产科就诊的子痫前期患者86例,根据患者的病情严重程度将其分为轻度子痫前期组(46例)和重度子痫前期组(40例),另选取同期在我院进行产检的健康孕妇40例作为对照组,比较三组受试者血清中APN、TNF-α、Apelin、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和总胆固醇(TC)水平,并分析子痫前期患者血清中APN、TNF-α、Apelin、LDL-C、TG、HDL-C、TC的相关性。结果:重度子痫前期组血清中APN水平低于轻度子痫前期组和对照组,TNF-α、Apelin水平高于轻度子痫前期组和对照组(P0.05);轻度子痫前期组血清中APN水平低于对照组,TNF-α、Apelin水平高于对照组(P0.05)。重度子痫前期组血清中LDL-C、TG、TC水平高于轻度子痫前期组和对照组,HDL-C水平低于轻度子痫前期组和对照组(P0.05);轻度子痫前期组LDL-C、TC水平高于对照组,HDL-C水平低于对照组(P0.05)。Pearson相关性分析结果显示,子痫前期患者血清中APN与TNF-α、Apelin、LDL-C、TG呈负相关,与HDL-C呈正相关(P0.05);TNF-α与Apelin、LDL-C、TG、TC呈正相关,与HDL-C呈负相关(P0.05);Apelin与LDL-C、TG、TC呈正相关,与HDL-C呈负相关(P0.05)。结论:子痫前期患者血清中APN水平明显下降,TNF-α、Apelin明显升高,三种指标可相互影响,且均可影响机体的脂质代谢水平,共同调节子痫前期患者的疾病进展。     

饲料中添加外源酶对大黄鱼和鲈氮磷排泄的影响

本研究以大黄鱼和鲈为实验对象,探讨饲料中添加植酸酶(PY)和非淀粉性多糖酶(WX和VP)对其氨氮和可溶性磷(PO3-4-P)排泄的影响.以含植物蛋白的饲料为基础饲料,分别向每千克饲料中添加200mg植酸酶(酶的活性为2500IU/g)、 800mg WX(主要包括葡聚糖酶、戊聚糖酶和纤维素酶,各种酶的活性皆为50IU/g)、 400mg VP(主要为木聚糖酶,酶的活性为1000IU/g)以及800mg WX 400mg VP配制出5种实验饲料.实验鱼在海水网箱中经过8周的摄食驯养后,转入室内水族箱中进行氮磷排泄测定实验.在水族箱中经2天的适应后,测定饥饿状态下氨氮及可溶性磷的排泄率.然后饱食投喂,并连续测定摄食后48h内鱼体氨氮和可溶性磷的排泄率.实验期间水温为26.5-32.5℃,盐度为32.5‰-36‰,溶氧在7 mg/L以上.实验结果表明,饥饿状态下实验鱼的氨氮和可溶性磷排泄不受实验饲料影响(p>0.05).而在饱食条件下,实验饲料中添加非淀粉性多糖酶(WX、VP及WX VP)显著降低了实验鱼的氨氮排泄率(p<0.05),而添加植酸酶组实验鱼的氨氮排泄率与对照组差异不显著.饲料处理对实验鱼可溶性磷的排泄率的影响不显著(p>0.05),但添加植酸酶组实验鱼的可溶性磷排泄率有增加的趋势.     

灰胸竹鸡消化系统形态解剖初探

观察和测定了13只灰胸竹鸡消化系统的解剖参数,结果表明:雌性和雄性体重分别为227.39 g和216.79 g,体长分别为237.22 mm和230.00 mm;腺胃粘膜表面具有40～50枚圆形乳头,肌胃较发达;雌性和雄性的食道分别长约93.96 mm和99.75 mm,肠道总长分别为683.83 mm和672.95 mm,肝脏分别重4.87 g和5.20 g,胰脏分别重0.38 g和0.45 g.此研究为进行竹鸡的人工驯养和生物学研究提供解剖学资料.     

具有一般形式饱和接触率SEIS模型的周期解

利用重合度的延拓定理,导出了具有一般形式饱和接触率SEIS模型周期解的存在性准则.     

猕猴桃不同季节的嫁接法比较试验

在湖北的试验表明,猕猴桃春季嫁接以切接法较好,夏秋季嫁接则以单芽枝腹接法较好;嫁接后采用遮阳网遮荫可以显著提高嫁接成活率,但对出圃率无明显影响。     

基于CdSe掺杂锐钛矿TiO_2的可见光体外灭活HL60细胞实验研究

用超声驱动方法合成了CdSe/TiO_2复合纳米粒子,并通过扫描电镜(SEM)、透射电镜(TEM)、X射线衍射(XRD)、紫外可见光(UV-Vis)吸收光谱和荧光(FS)光谱对CdSe/TiO_2复合纳米粒子进行表征。使用CCK-8法测定CdSe/TiO_2对白血病细胞的可见光光催化活性并通过SEM研究HL60细胞的表面超微结构形态。实验结果表明,用CdSe/TiO_2复合纳米粒子处理的组中观察到明显的HL60细胞生长抑制,并且HL60细胞在CdSe掺杂TiO_2复合纳米粒子作用下的PDT效率显著高于TiO_2,表明可以通过CdSe的修饰有效增强TiO_2的可见光光催化活性。此外,CdSe/TiO_2在可见光辐照下在4μg/m L的终值浓度下显示非常高的光动力效率,达76%。荧光光谱分析表明,CdSe/TiO_2复合纳米粒子可能通过分离光生空穴电子对提高此复合纳米粒子的光催化活性,从而提高CdSe/TiO_2对HL60细胞的PDT灭活效率。     

Overexpression of glutathione peroxidase increases the resistance of neuronal cells to Abeta-mediated neurotoxicity

Senile plaques are neuropathological manifestations in Alzheimer's disease (AD) and are composed mainly of extracellular deposits of amyloid beta-peptide (Abeta). Various data suggest that the accumulation of Abeta may contribute to neuronal degeneration and that Abeta neurotoxicity could be mediated by oxygen free radicals. Removal of free radicals by antioxidant scavengers or enzymes was found to protect neuronal cells in culture from Abeta toxicity. However, the nature of the free radicals involved is still unclear. In this study, we investigated whether the neuronal overexpression of glutathione peroxidase (GPx), the major hydrogen peroxide (H2O2)-de-grading enzyme in neurons, could increase their survival in a cellular model of Abeta-induced neurotoxicity. We infected pheochromocytoma (PC12) cells and rat embryonic cultured cortical neurons with an adenoviral vector encoding GPx (Ad-GPx) prior to exposure to toxic concentrations of Abeta(25-35) or (1-40). Both PC12 and cortical Ad-GPx-infected cells were significantly more resistant to Abeta-induced injury. These data strengthen the hypothesis of a role of H2O2 in the mechanism of Abeta toxicity and highlight the potential of Ad-GPx to reduce Abeta-induced damage to neurons. These findings may have applications in gene therapy for AD.     

How reticulated are species?

Many groups of closely related species have reticulate phylogenies. Recent genomic analyses are showing this in many insects and vertebrates, as well as in microbes and plants. In microbes, lateral gene transfer is the dominant process that spoils strictly tree‐like phylogenies, but in multicellular eukaryotes hybridization and introgression among related species is probably more important. Because many species, including the ancestors of ancient major lineages, seem to evolve rapidly in adaptive radiations, some sexual compatibility may exist among them. Introgression and reticulation can thereby affect all parts of the tree of life, not just the recent species at the tips. Our understanding of adaptive evolution, speciation, phylogenetics, and comparative biology must adapt to these mostly recent findings. Introgression has important practical implications as well, not least for the management of genetically modified organisms in pest and disease control.     

Sirtuin1:抗炎治疗新靶点

Sirtuin 1(SIRT1)是组蛋白去乙酰化酶的代表性成员,除可调节代谢、衰老、凋亡外,SIRT1还可通过催化组蛋白及核因子κB(NF-κB)、激活蛋白1(AP-1)等的去乙酰化,从而改变染色质构象、降低转录因子活性、下调炎症基因转录。临床研究已揭示SIRT1在某些炎症性疾病中含量明显降低,而动物实验证实白藜芦醇、SRT1720等SIRT1激活剂可有效减轻炎症损伤。因而,SIRT1有望成为抗炎治疗的新靶点。     

根黄酮在植物生长和营养中的作用

 本文简要综述了80年代以来国际上对根黄酮在调节植物根生长、完善根功能、影响氮素循环及在施加他感作用方面的研究进展,并对有关方面作了一些展望,以期引起植物营养工作者对植物次生物质的注意。