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871.
Javier Encinar del Dedo Fatima‐Zahra Idrissi Yolanda Arnáiz‐Pita Michael James Encarnación Dueñas‐Santero Sara Orellana‐Muñoz Francisco del Rey Vladimir Sirotkin M. Isabel Geli Carlos R. Vázquez de Aldana 《Traffic (Copenhagen, Denmark)》2014,15(10):1122-1142
Eng2 is a glucanase required for spore release, although it is also expressed during vegetative growth, suggesting that it might play other cellular functions. Its homology to the Saccharomyces cerevisiae Acf2 protein, previously shown to promote actin polymerization at endocytic sites in vitro, prompted us to investigate its role in endocytosis. Interestingly, depletion of Eng2 caused profound defects in endocytic uptake, which were not due to the absence of its glucanase activity. Analysis of the dynamics of endocytic proteins by fluorescence microscopy in the eng2Δ strain unveiled a previously undescribed phenotype, in which assembly of the Arp2/3 complex appeared uncoupled from the internalization of the endocytic coat and resulted in a fission defect. Strikingly also, we found that Eng2‐GFP dynamics did not match the pattern of other endocytic proteins. Eng2‐GFP localized to bright cytosolic spots that moved around the cellular poles and occasionally contacted assembling endocytic patches just before recruitment of Wsp1, the Schizosaccharomyces pombe WASP. Interestingly, Csh3‐YFP, a WASP‐interacting protein, interacted with Eng2 by co‐immunoprecipitation and was recruited to Eng2 in bright cytosolic spots. Altogether, our work defines a novel endocytic functional module, which probably couples the endocytic coat to the actin module. 相似文献
872.
Chaloin L Smagulova F Hariton-Gazal E Briant L Loyter A Devaux C 《Journal of biomedical science》2007,14(5):565-584
Summary Due to its essential role in the virus life cycle, the viral regulatory protein Rev constitutes an attractive target for the
development of new antiviral molecules. In this work, a series of Backbone Cyclic Peptide (BCP) analogs that bear a conformationally
constrained arginine rich motif (ARM) of Rev were tested for in vitro inhibition of HIV-1 replication. We observed a potent suppression of HIV-1 replication in chronically infected T lymphocytic
cells treated with Rev-BCPs. We further investigated possible mechanisms of HIV-1 inhibition and showed that Rev-BCPs interfere
slightly with the nuclear import process and are very efficient in blocking a mechanism that controls Pr55gag and gp160env synthesis. Interestingly, these protein precursors are known to be encoded by mRNAs that require Rev-binding for nuclear
export. In situ hybridization using a Cy-3 conjugated HIV-1 gag oligonucleotide probe indicated that Rev-BCPs prevent the intracellular accumulation
of unspliced viral RNA. As a model, the most promising analog, Rev-BCP 14, was studied by molecular modeling and dynamics
in order to identify its binding site on the Rev Response Element (RRE). The annealing simulation suggests that upon binding
on the RRE, Rev-BCP 14 widens the distorted major groove of the viral RNA. Numerous contacts between peptide and RNA were
found within the complex and some were identified as key components for the interactions. Altogether, our data indicate that
the use of conformationally constrained Rev-BCPs represents a promising strategy for the development of new peptide-based
therapeutic agents against HIV-1. 相似文献
873.
Nodal segments from field-grown culms were used as explants to develop a method of in vitro plantlet regeneration in Bambusa glaucescens Willd. through axillary bud proliferation. Shoot multiplication experiments were carried out with different concentrations
of benzyl adenine (BA) and kinetin (Kn), either singly or in combination. A synergistic effect of the two cytokinins was observed
and the best interaction giving the highest rate of shoot multiplication (4.00-fold) was obtained for a combination of 5 μM
BA and 15 μM Kn. The MS medium supplemented with 25 μM indole butyric acid (IBA) was most suitable for rooting of shoots.
Hardening and acclimatization was successful and plantlets are growing normally in soil. 相似文献
874.
875.
Hammouda F Messaoudi I El Hani J Baati T Saïd K Kerkeni A 《Biological trace element research》2008,126(1-3):194-203
The aim of this study was to evaluate the potential benefit of combined treatment with zinc (Zn) and selenium (Se) in reversing cadmium (Cd)-induced thyroid dysfunction compared to Se or Zn treatment alone in rats exposed to Cd. For this purpose, 30 adult male Wistar albino rats were equally divided into control and four treated groups receiving either 200 ppm Cd (as CdCl2), 200 ppm Cd + 500 ppm Zn (as ZnCl2), 200 ppm Cd + 0.1 ppm Se (as Na2SeO3), or 200 ppm Cd + 500 ppm Zn + 0.1 ppm Se in their drinking water for 35 days. The results showed that Cd exposure increased significantly the relative thyroid weight (RTW), the thyroid Cd concentration, and the serum thyroid stimulating hormone (TSH) level, whereas the serum thyroxine (T4) level was decreased compared to control rats. The treatment of Cd-exposed rats with Se alone only partially protected from the Cd-induced decrease in serum T4 level. The treatment of Cd-exposed animals with Zn alone partially protected against Cd-induced thyroid dysfunction by maintaining normal RTW and by decreasing Cd concentration in the thyroid. It also partially prevents Cd-induced decrease in serum T4 level. The combined treatment of Cd-exposed animals with Se and Zn induced a more significant decrease in the thyroid Cd concentration than the Zn supplement and a total correction of the RTW. This treatment was also more effective than that with Se or Zn alone in reversing Cd-induced decrease in serum T4 level and Cd-induced increase in serum TSH level. Se and Zn can have a synergistic role against Cd-induced thyroid dysfunction. 相似文献
876.
Background
The phospholipase D (PLD) family has been identified in plants by recent molecular studies, fostered by the emerging importance of plant PLDs in stress physiology and signal transduction. However, the presence of multiple isoforms limits the power of conventional biochemical and pharmacological approaches, and calls for a wider application of genetic methodology. 相似文献877.
Al Safar HS Abidi FH Khazanehdari KA Dadour IR Tay GK 《Applied microbiology and biotechnology》2011,89(3):807-815
In the field of epidemiology, Genome-Wide Association Studies (GWAS) are commonly used to identify genetic predispositions
of many human diseases. Large repositories housing biological specimens for clinical and genetic investigations have been
established to store material and data for these studies. The logistics of specimen collection and sample storage can be onerous,
and new strategies have to be explored. This study examines three different DNA sources (namely, degraded genomic DNA, amplified
degraded genomic DNA and amplified extracted DNA from FTA card) for GWAS using the Illumina platform. No significant difference
in call rate was detected between amplified degraded genomic DNA extracted from whole blood and amplified DNA retrieved from
FTA™ cards. However, using unamplified–degraded genomic DNA reduced the call rate to a mean of 42.6% compared to amplified
DNA extracted from FTA card (mean of 96.6%). This study establishes the utility of FTA™ cards as a viable storage matrix for
cells from which DNA can be extracted to perform GWAS analysis. 相似文献
878.
Surendra Singh Bisht Seerat Fatima Akhilesh K. Tamrakar Neha Rahuja Natasha Jaiswal Arvind K. Srivastava Rama P. Tripathi 《Bioorganic & medicinal chemistry letters》2009,19(10):2699-2703
A simple synthesis of phenyl butenoyl C-glycosides has been achieved by Aldol condensation of peracetylated glycosyl acetones with aromatic aldehydes followed by deacetylation with methanolic NaOMe. The selected butenoyl C-glycosides on conjugate addition of diethyl malonate resulted in polyfunctional alkanonyl glycosides in good yields. The butenoyl C- and alkanoyl C-glycosides were evaluated for their α-glucosidase, glucose-6-phosphatse and glycogen phosphorylase enzyme inhibitory activities in vitro. Three of the synthesized (3, 5 and 9) showed potent enzyme inhibitory activities as compared to standard drugs. Compounds 3, 5 and 9 were evaluated in vivo too displaying significant activity as compared to standard drugs acarbose and metformin. 相似文献
879.
Fatima Bousejra-El Garah Marguerite Pitié Laure Vendier Bernard Meunier Anne Robert 《Journal of biological inorganic chemistry》2009,14(4):601-610
The reductive activation of artemisinin by copper(I)-dipyrrin or copper(I)-(2-Clip-Phen) complexes generates an artemisinin
derived alkylating species leading to covalent artemisinin–copper complex adducts. The reactivity of the peroxide function
of artemisinin toward Cu(I) complexes is similar to that of Fe(II) analogues, even though the reaction is more sluggish and
product distribution slightly different. 相似文献
880.