全文获取类型
收费全文 | 83篇 |
免费 | 10篇 |
出版年
2021年 | 4篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 6篇 |
2012年 | 3篇 |
2011年 | 3篇 |
2010年 | 6篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 8篇 |
2006年 | 1篇 |
2005年 | 2篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1982年 | 1篇 |
1977年 | 1篇 |
1970年 | 1篇 |
1957年 | 2篇 |
1951年 | 1篇 |
排序方式: 共有93条查询结果,搜索用时 15 毫秒
51.
52.
Background
To infer homology and subsequently gene function, the Smith-Waterman (SW) algorithm is used to find the optimal local alignment between two sequences. When searching sequence databases that may contain hundreds of millions of sequences, this algorithm becomes computationally expensive. 相似文献53.
F. Natali L. Cecchi T. Torrigiani Malaspina F. Barbano S. Orlandini 《Aerobiologia》2013,29(3):399-406
The aim of this study was to assess the current aerobiological situation and to investigate the influence of the hot and dry summer 2003 on pollen season (onset, end and duration of the pollen season, peak pollen day and value, total seasonal amount of pollen grains) of herbaceous family as Poaceae, Urticaceae and Compositeae. Heat wave of 2003 influenced the phenology of the main pollen families in Tuscany: the high temperatures occurred during 2003 affected pollen season of different family with different responses. This study confirms the role that the climate has on the flora species and in particular on herbaceous species phenology and the high variability of the pollination among different places, during extreme events. In general, high spring temperature induces an advance of the flowering period and a release of higher pollen quantity of Urticaceae and Poaceae; however, exceptional weather conditions (i.e., summer 2003) could exert an opposite effect, resulting in an impairment of flowering of Urticaceae during autumn. Compositeae species produced a low amount of pollen in 2003, even if the peak value was higher than the average in some stations. 相似文献
54.
Kumaran Kandasamy Sujatha S Mohan Rajesh Raju Shivakumar Keerthikumar Ghantasala S Sameer Kumar Abhilash K Venugopal Deepthi Telikicherla Daniel J Navarro Suresh Mathivanan Christian Pecquet Sashi Kanth Gollapudi Sudhir Gopal Tattikota Shyam Mohan Hariprasad Padhukasahasram Yashwanth Subbannayya Renu Goel Harrys KC Jacob Jun Zhong Raja Sekhar Vishalakshi Nanjappa Lavanya Balakrishnan Roopashree Subbaiah YL Ramachandra Abdul B Rahiman Keshava TS Prasad Jian-Xin Lin Jon CD Houtman Stephen Desiderio Jean-Christophe Renauld Stefan N Constantinescu Osamu Ohara Toshio Hirano Masato Kubo Sujay Singh Purvesh Khatri Sorin Draghici Gary D Bader Chris Sander Warren J Leonard Akhilesh Pandey 《Genome biology》2010,11(1):1-9
55.
A new variant of glucose phosphate isomerase (GPI), also known as phosphohexose isomerase (PHI), was detected in a primitive pig population. 相似文献
56.
57.
Jon Beck Mark Poulter Davina Hensman Jonathan?D. Rohrer Colin J. Mahoney Gary Adamson Tracy Campbell James Uphill Aaron Borg Pietro Fratta Richard?W. Orrell Andrea Malaspina James Rowe Jeremy Brown John Hodges Katie Sidle James?M. Polke Henry Houlden Jonathan?M. Schott Nick?C. Fox Martin?N. Rossor Sarah?J. Tabrizi Adrian?M. Isaacs John Hardy Jason D. Warren John Collinge Simon Mead 《American journal of human genetics》2013,92(3):345-353
Hexanucleotide repeat expansions in C9orf72 are a major cause of frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). Understanding the disease mechanisms and a method for clinical diagnostic genotyping have been hindered because of the difficulty in estimating the expansion size. We found 96 repeat-primed PCR expansions: 85/2,974 in six neurodegenerative diseases cohorts (FTLD, ALS, Alzheimer disease, sporadic Creutzfeldt-Jakob disease, Huntington disease-like syndrome, and other nonspecific neurodegenerative disease syndromes) and 11/7,579 (0.15%) in UK 1958 birth cohort (58BC) controls. With the use of a modified Southern blot method, the estimated expansion range (smear maxima) in cases was 800–4,400. Similarly, large expansions were detected in the population controls. Differences in expansion size and morphology were detected between DNA samples from tissue and cell lines. Of those in whom repeat-primed PCR detected expansions, 68/69 were confirmed by blotting, which was specific for greater than 275 repeats. We found that morphology in the expansion smear varied among different individuals and among different brain regions in the same individual. Expansion size correlated with age at clinical onset but did not differ between diagnostic groups. Evidence of instability of repeat size in control families, as well as neighboring SNP and microsatellite analyses, support multiple expansion events on the same haplotype background. Our method of estimating the size of large expansions has potential clinical utility. C9orf72-related disease might mimic several neurodegenerative disorders and, with potentially 90,000 carriers in the United Kingdom, is more common than previously realized. 相似文献
58.
Diana Vasquez-Cardenas Jack van de Vossenberg Lubos Polerecky Sairah Y Malkin Regina Schauer Silvia Hidalgo-Martinez Veronique Confurius Jack J Middelburg Filip JR Meysman Henricus TS Boschker 《The ISME journal》2015,9(9):1966-1978
Recently, a novel electrogenic type of sulphur oxidation was documented in marine sediments, whereby filamentous cable bacteria (Desulfobulbaceae) are mediating electron transport over cm-scale distances. These cable bacteria are capable of developing an extensive network within days, implying a highly efficient carbon acquisition strategy. Presently, the carbon metabolism of cable bacteria is unknown, and hence we adopted a multidisciplinary approach to study the carbon substrate utilization of both cable bacteria and associated microbial community in sediment incubations. Fluorescence in situ hybridization showed rapid downward growth of cable bacteria, concomitant with high rates of electrogenic sulphur oxidation, as quantified by microelectrode profiling. We studied heterotrophy and autotrophy by following 13C-propionate and -bicarbonate incorporation into bacterial fatty acids. This biomarker analysis showed that propionate uptake was limited to fatty acid signatures typical for the genus Desulfobulbus. The nanoscale secondary ion mass spectrometry analysis confirmed heterotrophic rather than autotrophic growth of cable bacteria. Still, high bicarbonate uptake was observed in concert with the development of cable bacteria. Clone libraries of 16S complementary DNA showed numerous sequences associated to chemoautotrophic sulphur-oxidizing Epsilon- and Gammaproteobacteria, whereas 13C-bicarbonate biomarker labelling suggested that these sulphur-oxidizing bacteria were active far below the oxygen penetration. A targeted manipulation experiment demonstrated that chemoautotrophic carbon fixation was tightly linked to the heterotrophic activity of the cable bacteria down to cm depth. Overall, the results suggest that electrogenic sulphur oxidation is performed by a microbial consortium, consisting of chemoorganotrophic cable bacteria and chemolithoautotrophic Epsilon- and Gammaproteobacteria. The metabolic linkage between these two groups is presently unknown and needs further study. 相似文献
59.
60.