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81.
Isolation of virus causing hemorrhagic fever with renal syndrome (HFRS) through a cell culture system 总被引:4,自引:0,他引:4
T Kitamura C Morita T Komatsu K Sugiyama J Arikawa S Shiga H Takeda Y Akao K Imaizumi A Oya N Hashimoto S Urasawa 《Japanese journal of medical science & biology》1983,36(1):17-25
Twenty-three rat lung specimens collected in outbreaks of hemorrhagic fever with renal syndrome (HFRS) in three medical institutions were inoculated onto the VERO-E6 cell monolayers. After several blind passages, an agent growing serially in the cell cultures and reacting specifically with known HFRS-positive sera was isolated from two of these specimens. The two isolates were antigenically identical each other. The agent, named strain SR-11, was identified as the causative virus of HFRS by its antigenic identity with E6 cell-adapted HFRS virus, Hantaan 76-118 strain, and the specific reactions with sera from various HFRS cases. 相似文献
82.
Takeda Junko; Abe Shunnosuke; Morikawa Hiromichi; Senda Mitsugi 《Plant & cell physiology》1983,24(4):667-676
The electrophysiological properties of the membrane of Nicotianatabacum var. Sarnsun cultured cells were determined using amicroelectrode technique in standard medium containing 1 mMKC1, 1 mM NaCl and 1 mu CaCl2 at pH 7. Tobacco callus was derivedfrom the pith (Em=104.4%16.2 mV). The membrane potentialsof the callus cells did not show a symmetrical Gaussian distributionbut were scattered over a wide range. The percentage of highmembrane potential cells increased as the subculture was continueduntil about 11 months and then decreased. The response of themembrane potential to electric stimulus, ionic composition,metabolic inhibitors, sugars and amino acids was characteristicof high (Em={small tilde}160 220 mV; H-cells)and low (Em=80{small tilde}90 mV; L-cells) membranepotential cells. The membrane potential of H-cells was largelydepolarized by addition of CN, carbonium cyanide m-chlorophenylhydrazone,decyclohexylcarbodiimide, and triphenyltin chloride and transientlydepolarized by addition of glucose, galactose, mannose or sucrose,and D-alanine, L-alanine or Llysine, but the membrane potentialof L-cells was not. (Received December 3, 1982; Accepted March 16, 1983) 相似文献
83.
Makoto Hayashi Kazuko Fukushima Akihide Kitamura 《Chemistry and physics of lipids》1983,33(3):233-239
Krafft points of diacylglycerophosphocholines (PC) were measured in alkanes-cyclohexane solutions by differential scanning calorimetry, and it was found that they were regularly increased following the increase in alkane content in the solutions and the chain length of the alkanes. From these results it was deduced that the mixing of PC with alkanes occurred in the gel state of the PC, but not in micelles at higher temperatures above the Krafft points. where micellar solutions are provided. The penetration of alkanes into gel state PC was found to be dominated by Langmuir type interaction, and the affinity of alkanes increases with increasing in chain lengths. Above the Krafft points, the micelle formation was confirmed by using the fluorescence probe technique. 相似文献
84.
Nuclear phosphoprotein kinases from rat liver 总被引:3,自引:0,他引:3
85.
86.
Studies on ATP citrate lyase of rat liver. IV. The role of CoA 总被引:1,自引:0,他引:1
87.
88.
A thermolabile direct hemolysin of Vibrio parahaemolyticus 总被引:6,自引:0,他引:6
89.
90.