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221.
The flow properties of soybean protein–lipid–water suspension systems and coagulated gels were related to a protein–lipid interaction. For powdered soybean lecithin-added soybean protein suspension systems and their heat-induced gels, the yield stress (σy) and the consistency index (K) increased with increasing amounts of added lipid, but the flow behavior index (n) and the thixotropy index (TI) decreased. On the other hand, there were only small changes in the magnitudes of the thixotropic parameters and the viscometric parameters (σy, K and n) after adding soybean oil at various concentrations to soybean protein dispersions. These facts suggested that the formation of a protein–phospholipid complex increased the effective particle size, and that the intermolecular entanglements and linkages among the protein molecules or among the protein-phospholipid complexes were weakened by the addition of polar lipids. The thixotropy index defined in this study is available for characterizing the stress decay that occurs within soybean protein dispersions and heat-induced gels as they are sheared. 相似文献
222.
Four components of ovomucoid were digested exhaustively and four kinds of glycopeptide corresponding to the four components were separated by gel filtration. Each glycopeptide was shown to be homogenious by paper chromatography and paper electrophoresis. Molar ratios of carbohydrate components of these glycopeptides varied to some extent but the amino acid compositions of these glycopeptides were essentially identical with each other with the exception of alanine. Aspartic acid and threonine were predominant amino acids in the all glycopeptides. It is most likely that the modes of linkages between polysaccharide and protein in individual ovomucoid I, II, III and IV are essentially the same, and that the carbohydrate moiety is linked to the protein via asparaginyl residue or the hydroxyl group of threonine, although the possibility of the linkages to glutamine and serine can not be excluded. 相似文献
223.
Makoto Takeuchi Eisuke Tsuda Masaaki Yoshikawa Ryuzo Sasaki Hideo Chiba 《Bioscience, biotechnology, and biochemistry》2013,77(11):2789-2797
Bovine κ-casein, a phosphoglycoprotein, has mucin-type carbohydrate chains. Subcellular distribution of enzymes that take part in the post-translational modification of κ-casein was examined. In lactating mammary glands from rats and cows, N-acetyl-galactosaminyl transferase, galactosyl transferase, sialyl transferase, and casein kinase were localized specifically in the Golgi apparatus.The substrate specificities indicate that these enzymes are actually responsible for the processing of κ-casein.The presence of a phosphate group attached to κ-casein did not affect the rate of glycosylation by N-acetyl-galactosaminyl transferase, while the presence of carbohydrate chains attached to κ- casein strongly reduced the rate of phosphorylation by casein kinase. These results suggest that in the Golgi apparatus, phosphorylation of κ-casein precedes glycosylation. 相似文献
224.
Jun Aketagawa Kimiko Kojo Makoto Ishimoto 《Bioscience, biotechnology, and biochemistry》2013,77(8):2359-2365
The sulfite reductase of Desulfovibrio vulgaris, strain Miyazaki F (MF), was purified by ammonium sulfate precipitation and chromatography on DEAE-cellulose, Ultrogel AcA34, and hydroxylapatite. The molecular weight was estimated to be 180,000 by gel filtration. It had a subunit structure of α2β2; the molecular weight of the α subunit was 50,000 and that of β, 39,000. The absorption spectrum with characteristic peaks at 629 and 409 nm and the amino acid composition resembled those of the sulfite reductase from D. vulgaris, Miyazaki K. The MF enzyme reduced sulfite to trithionate, thiosulfate, and sulfide by hydrogen when coupled with a hydrogenase-methyl viologen system, like other sulfite reductases from Desulfovibrio. 相似文献
225.
Saburo Tamura Makoto Takai Satoshi Miyamoto Seiroku Sakai Masayoshi Goda Yusuke Sumiki 《Bioscience, biotechnology, and biochemistry》2013,77(10):773-777
Various O,O-dialkyl O-cyanophenyl phosphates and phosphorothioates were prepared and their biological activities were examined. Among them, O,O-dimethyl O- (4-chloro-2-cyanophenyl) phosphorothioate was found to have selective and high toxicity to houseflies. O,O-Dimethyl O- (4-cyanophenyl) phosphorothioate, O,O-diethyl O- (4-cyanophenyl) phosphorothioate and O,O-diethyl O- (2-chloro-4-cyanophenyl) phosphorothioate showed high insecticidal activty to American cockroaches, though the former two were not so effective to houseflies. The dimethyl esters of these series exhibited markedly lowered mammalian toxicity. Among the O-ethyl O-cyanophenyl phenylphosphonothioates, O-ethyl O- (2-chloro-4-cyanophenyl) phenylphosphonothioate was highly effective to mites, while less effective to insects. 相似文献
226.
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228.
Hajime Taniguchi Fumihiko Odashima Makoto Igarashi Yoshiharu Maruyama Michinori Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(8):2107-2115
A bacterium which can utilize potato starch granules as sole carbon source was isolated and identified as Bacillus circulans from its physiological and biochemical properties. Scanning electron microscopic observation of potato starch granules recovered from the culture broth revealed that granules were degraded gradually from their surface resulting in elongated granules with layered structures on their surface. This bacterium produced extracellular amylase which can digest potato starch granules in vitro. The amylase has a unique property in that it produces only maltohexaose from gelatinized starch in the early stage of the reaction. For the production of this amylase potato starch was found to be most effective while soluble sugars including gelatinized starch and maltose had little effect. 相似文献
229.
Shin Sato Kin-ichi Nakamura Makoto Tadenuma 《Bioscience, biotechnology, and biochemistry》2013,77(3):308-313
Coloring reaction of sake caused by exposure to sun-light was investigated. There is one of the coloring reactions in which deferri-ferrichrysin participate. The reaction was named Reaction I.Deferri-ferrichrysin, tyrosine, Mn2+ and unknown nitrogenous compound named conveniently compound X were essential for Reaction I and citric acid was stimulative.Compound X was purified by using Amberlite IR 120 (H type) column, active carbon column, silicic acid column and alumina column, and crystallized from methanol-water (1 : 1).The crystals decomposed at 288~290°C. The ultraviolet and infrared spectra of the compound X were essentially identical with those of authentic kynurenic acid.From these results the compound X was identified as kynurenic acid.Since kynurenic acid alone did not cause the color development and riboflavin could be substituted for kynurenic acid in Reaction I, kynurenic acid may act as photosensitizer in Reaction I. 相似文献
230.
Soichi Arai Makoto Abe Michiko Yamashita Hiromichi Kato Masao Fujimaki 《Bioscience, biotechnology, and biochemistry》2013,77(4):552-559
An indole derivative having blue fluorescence was produced in cooked soybean digested at 37°C for 24 hr with an acid proteinase Molsin (optimum pH: 2.8) from Aspergillus saitoi or a usual acid proteinase pepsin (optimum pH: 1.6) from beef stomach. This indole derivative was identical with a condensation product from l-tryptophan and n-hexanal. Based on MS, NMR, IR and UV spectrometry, the condensation product was identified as l-pentyl-2, 3, 4, 9-tetrahydro-lH-pyrido [3, 4-b]-indole-3-carboxylic acid [trivial name: 1-pentyl-l, 2, 3, 4-tetrahydro-2-carboline carboxylic acid-(3)].Data were presented of the formation of the above indole derivative and of the resulting consumption of l-tryptophan and n-hexanal.The possible ocurrence of the formation of Harmala alkaloids, i.e. 2-carboline derivatives, through in vitro digestion of soybean with acid proteinases was discussed.A carbonyl-trapping ability of l-tryptophan was suggested. 相似文献