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21.
V. G. Dvoretsky M. P. Venger P. R. Makarevich D. V. Moiseev 《Russian Journal of Marine Biology》2012,38(1):91-95
The distribution of bacterio- and zooplankton was studied in West Spitsbergen (Svalbard) coastal waters in August 2009. The
bacterial abundance and biomass varied from 110 to 3360 × 103 cells/ml and from 0.66 to 44.93 mg C/m3, respectively. These values were the highest in the surface water layers and decreased with depth. The greatest abundance
of bacteria (5099 individuals/m3) was found in the freshened and warm water layer in Isfjorden. The highest biomass of zooplankton
(182.41 mg C/m3) was registered in the zone of interaction of coastal and transformed Atlantic waters in the archipelago inner shelf. The
spatial variations of abundance and biomass of the planktonic communities were related to the thermohaline characteristics
of the water masses and, probably, to microalgal blooms. 相似文献
22.
Usdin K; Chevret P; Catzeflis FM; Verona R; Furano AV 《Molecular biology and evolution》1995,12(1):73-82
The single most difficult problem in phylogenetic analysis is deciding
whether a shared taxonomic character is due to common ancestry or one that
appeared independently due to convergence, parallelism, or reversion to an
ancestral state. Mammalian L1 retrotransposons undergo periodic
amplifications in which multiple copies of the elements are interspersed in
the genome. Because these elements apparently are transmitted only by
inheritance and are retained in the genome, a shared L1 amplification event
can only be an inherited ancestral character. We propose that L1
amplification events can be an excellent tool for analyzing mammalian
evolution and demonstrate here how we addressed several refractory problems
in rodent systematics using L1 DNA as a taxonomic character.
相似文献
23.
The seasonal dynamics of biomass and size structure of nanophytoplankton in Central Murman (off the southern coast of the Barents Sea) are described according to the results of six-year continuous observations. The pattern of the annual successional cycle indicates that the nanophytoplankton community as a structural unit of coastal pelagic algocenoses is a specific integrated ecological group of primary producers. The issue of a major contribution of nanoalgae to the total biomass and production of the autotrophic component of coastal ecosystems in Arctic seas is discussed. 相似文献
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26.
Yu. I. Goryaev N. G. Chelintsev A. V. Ezhov P. R. Makarevich D. G. Ishkulov 《Doklady biological sciences》2017,474(1):114-119
In August–September 2015 in the southwest part of the Kara Sea, visual censuses of ringed seal (Phoca hispida) and bearded seal (Erignatus barbatus) from research vessels were carried out in the water area of 49 814 km2. For the first time, for calculation of abundance and density on ice-free area the SEAL-CALCULATION (TYULEN-RASCHET) software was used with application of separate extrapolation method by the selected sectors. Calculation for the whole census polygon yielded abundance of ringed seal of 4163 ± 16.9% and abundance of bearded seal of 1026 ± 28.4%. On the basis of the census results, a preliminary estimation of distribution of pinnipeds by various depths is presented. 相似文献
27.
Konstantin V. Dergilev Pavel I. Makarevich Zoya I. Tsokolaeva Maria A. Boldyreva Irina B. Beloglazova Ekaterina S. Zubkova Mikhail Yu. Menshikov Yelena V. Parfyonova 《Tissue & cell》2017,49(1):64-71
Cell sheets (CS) from c-kit+ cardiac stem cell (CSC) hold a potential for application in regenerative medicine. However, manufacture of CS may require thermoresponsive dishes, which increases cost and puts one in dependence on specific materials. Alternative approaches were established recently and we conducted a short study to compare approaches for detachment of CS from c-kit+ CSC. Our in-house developed method using chelation by Versene solution was compared to UpCell? thermoresponsive plates in terms of CSC proliferation, viability, gap junction formation and engraftment in a model of myocardial infarction. Use of Versene solution instead of thermoresponsive dishes resulted in comparable CS thickness (approximately 100 mcm), cell proliferation rate and no signs of apoptosis detected in both types of constructs. However, we observed a minor reduction of gap junction count in Versene-treated CS. At day 30 after delivery to infarcted myocardium both types of CS retained at the site of transplantation and contained comparable amounts of proliferating cells indicating engraftment. Thus, we may conclude that detachment of CS from c-kit+ CSC using Versene solution followed by mechanical treatment is an alternative to thermoresponsive plates allowing use of routinely available materials to generate constructs for cardiac repair. 相似文献
28.
R Alan Harris Dorottya Nagy-Szakal Sabina AV Mir Eibe Frank Reka Szigeti Jess L Kaplan Jiri Bronsky Antone Opekun George D Ferry Harland Winter Richard Kellermayer 《Epigenetics》2014,9(8):1131-1137
Inflammatory bowel diseases (IBD) are emerging globally, indicating that environmental factors may be important in their pathogenesis. Colonic mucosal epigenetic changes, such as DNA methylation, can occur in response to the environment and have been implicated in IBD pathology. However, mucosal DNA methylation has not been examined in treatment-naïve patients. We studied DNA methylation in untreated, left sided colonic biopsy specimens using the Infinium HumanMethylation450 BeadChip array. We analyzed 22 control (C) patients, 15 untreated Crohn’s disease (CD) patients, and 9 untreated ulcerative colitis (UC) patients from two cohorts. Samples obtained at the time of clinical remission from two of the treatment-naïve UC patients were also included into the analysis. UC-specific gene expression was interrogated in a subset of adjacent samples (5 C and 5 UC) using the Affymetrix GeneChip PrimeView Human Gene Expression Arrays. Only treatment-naïve UC separated from control. One-hundred-and-twenty genes with significant expression change in UC (> 2-fold, P < 0.05) were associated with differentially methylated regions (DMRs). Epigenetically associated gene expression changes (including gene expression changes in the IFITM1, ITGB2, S100A9, SLPI, SAA1, and STAT3 genes) were linked to colonic mucosal immune and defense responses. These findings underscore the relationship between epigenetic changes and inflammation in pediatric treatment-naïve UC and may have potential etiologic, diagnostic, and therapeutic relevance for IBD. 相似文献
29.
Human brain cDNA libraries were screened with cDNA inserts corresponding to the mRNA for the Na+,K(+)-ATPase alpha-subunit from pig kidney. The results obtained demonstrate the existence of two highly homologous mRNAs encoding the alpha- and alpha III-isoforms of the Na+,K(+)-ATPase catalytic subunit. 相似文献