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61.
Azarin Kirill Usatov Alexander Makarenko Maksim Kozel Nikolay Kovalevich Alexey Dremuk Irina Yemelyanova Anna Logacheva Mariya Fedorenko Aleksei Averina Nataliya 《Plant molecular biology》2020,102(4-5):373-388
Plant Molecular Biology - CRISPR-edited variants at the 3′-end of OsLOGL5's coding sequence (CDS), significantly increased rice grain yield under well-watered, drought, normal nitrogen,... 相似文献
62.
S. P. Makarenko L. V. Dudareva A. I. Katyshev T. A. Konenkina A. V. Nazarova E. G. Rudikovskaya N. A. Sokolova V. V. Chernikova Yu. M. Konstantinov 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2011,5(1):64-69
The study was focused on fatty acid (FA) composition of lipids from the seedlings and roots of crops having different cold
resistance and grown at 27°C or 4°C. Biosynthesis of FA in the lipids of seedlings and roots of cold-susceptible maize (Zea mays L.) at both growth temperatures was controlled by chloroplast ω6 desaturase and microsomal ω6 desaturase, respectively. The
content of linoleic acid was 56.2% and 43.3% in the coleoptiles of maize seedlings grown at 4 and 27°C, respectively, and
in the roots it was 52.2% and 38.5%, respectively. The content of α-linolenoic acid in the coleoptiles was 6.7–6.8% at both
temperatures, while in the root lipids it was higher at low temperature (3.15% at 27°C vs. 4.7% at 4°C). FA biosynthesis in
the seedling coleoptiles of wheat (Triticum aestivum L.) and Siberian wild rye (Elymus sibiricus L.) grown at low temperature was controlled by the chloroplast ω3 desaturase. A minor increase in the content of α-linolenoic
acid was observed at low temperature: 29.7% to 30.2% in wheat and 22.8% to 25.8% in wild rye. In the root tissues of these
species, the biosynthesis of α-linolenoic acid was controlled by the microsomal ω3 desaturase. The content of α-linolenoic
acid was higher at low temperature: in wheat it was 6.1% at 27°C and 17.1%, and 4°C, while in Siberian wild rye, 7.1% and
12.0% at 4 and 27°C, respectively. 相似文献
63.
The influence of examination stress on the reactions of the autonomic nervous system in students with different levels of functional mobility of nervous processes (FMNP) was studied. More intense functioning of the cardiovascular system and the strain of all regulatory mechanisms during examination stress were characteristic of individuals with low FMNP, whereas students with high FMNP values were characterized by a weaker strain of the mechanisms of cardiac rhythm regulation, more economical activity of the cardiovascular system, and better performance on the examinations. The activation of humoral, metabolic, and sympathetic effects was observed in all the subjects under examination stress, along with decreased parasympathetic effects on the cardiac rhythm. 相似文献
64.
L S Edvabnaia I G Za?dner T A Makarenko V F Bulk M I Zhvanetskaia 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(11):18-23
Partially purified water extract was obtained from the initial water extract by ultracentrifugation. Nine protein fractions differing in molecular weight, homogeneity and the content of lipopolysaccharide (LPS) were obtained by stepwise precipitation with ammonium sulfate and subsequent fractionation in columns packed with Sephadex G-100 and DEAE cellulose. Two protein fractions with a molecular weight of 30000 and 40000 daltons were practically free of LPS. These fractions were homogeneous as shown by analytical centrifugation and formed a single precipitation line with P. aeruginosa antiserum; both fractions were found to be antigenically identical. In the enzyme immunoassay these two fractions proved to be least active in comparison with the other protein fractions, but when used for the immunization of rabbits, they induced the formation of specific protective (for mice) antibodies. Both antisera were equally active in the experiments of the passive protection of mice. The isolated LPS-free proteins are supposed to be the proteins of the outer membrane of P. aeruginosa cell wall and have the properties of protective antigens. 相似文献
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We studied the development of direct axonal connections of the accessory neurosecretory hypothalamic nuclei with the posterior pituitary lobe on the fixed rat brain from day 15 of embryogenesis until day 10 of postnatal development using the retrograde diffusion method of the lipophilic fluorescent carbocyanine dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate along the neuronal membranes. The marker was applied onto the posterior pituitary lobe and, after incubation in a fixative, fluorescing bodies of nerve cells were visualized in the hypothalamus. Neuronal axons of the retrochiasmatic nucleus were the first of the accessory nuclei to ingrow in the posterior pituitary lobe (on days 16-17 of embryogenesis). Neurons of the circular and dorsolateral nuclei and the nuclei of the median bundle of the forebrain sent their axons to the posterior pituitary lobe starting from the first days of postnatal development. No direct connections of the anterior commissure and perifornical accessory nuclei with the posterior pituitary lobe were found in perinatal development. These facts are discussed in the light of concepts about the different functional role of accessory peptidergic hypothalamic nuclei in rats. 相似文献
68.
Development of Biosensors for Phenol Determination from Bacteria Found in Petroleum Fields of West Siberia 总被引:2,自引:0,他引:2
Makarenko A. A. Bezverbnaya I. P. Kosheleva I. A. Kuvichkina T. N. Ilyasov P. V. Reshetilov A. N. 《Applied Biochemistry and Microbiology》2002,38(1):23-27
Nine gram-negative bacterial strains, selected from 300 strains isolated from soils of the West Siberian petroliferous basin and growing on oil and oil products, consume phenol as a single carbon and energy source. The strains were used for the development of a sensor bioreceptor. The most active 32-I strain was shown to bear a plasmid responsible for phenol degradation. The plasmid-free derivative of this strain, 32-I-1, did not grow on phenol. The possibility of creating a model biosensor for phenol based on the plasmid-containing 32-I strain is considered. The detection limit for phenol was 5 M. The optimum conditions for the sensor operation are: pH 7.4, 35°C, and operation time 30 h. 相似文献
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70.
The effect of astasilid, a sucrose monoester and the effect of mainly unsaturated fatty acids from the lipid fraction of Astasia longa on immunocompetent cells--macrophages of the mouse peritoneal cavity were studied. It was shown that astasilid increased 7.5-8.5-fold expression of Fc-receptors on the macrophage plasmic membranes and stimulated 5.5-6.5-fold the macrophage capacity for Fc-dependent phagocytosis of sheep red blood cells. Astasilid had no effect on migration of the macrophages into the abdominal cavity. 相似文献