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排序方式: 共有927条查询结果,搜索用时 328 毫秒
361.
Qing Li Ying Wang Qiang Sun Jasmin Knopf Martin Herrmann Liangyu Lin Jingting Jiang Changshun Shao Peishan Li Xiaozhou He Fei Hua Zubiao Niu Chaobing Ma Yichao Zhu Giuseppe Ippolito Mauro Piacentini Jerome Estaquier Sonia Melino Felix Daniel Weiss Emanuele Andreano Eicke Latz Joachim L. Schultze Rino Rappuoli Alberto Mantovani Tak Wah Mak Gerry Melino Yufang Shi 《Cell death and differentiation》2022,29(6):1107
The coronavirus disease 2019 (COVID-19) has been a global pandemic for more than 2 years and it still impacts our daily lifestyle and quality in unprecedented ways. A better understanding of immunity and its regulation in response to SARS-CoV-2 infection is urgently needed. Based on the current literature, we review here the various virus mutations and the evolving disease manifestations along with the alterations of immune responses with specific focuses on the innate immune response, neutrophil extracellular traps, humoral immunity, and cellular immunity. Different types of vaccines were compared and analyzed based on their unique properties to elicit specific immunity. Various therapeutic strategies such as antibody, anti-viral medications and inflammation control were discussed. We predict that with the available and continuously emerging new technologies, more powerful vaccines and administration schedules, more effective medications and better public health measures, the COVID-19 pandemic will be under control in the near future. Subject terms: Infectious diseases, Antimicrobial responses 相似文献
362.
Rearrangement of antigen receptor genes is defective in mice with severe combined immune deficiency 总被引:101,自引:0,他引:101
W Schuler I J Weiler A Schuler R A Phillips N Rosenberg T W Mak J F Kearney R P Perry M J Bosma 《Cell》1986,46(7):963-972
A process unique to lymphocyte differentiation is the rearrangement of genes encoding antigen-specific receptors on B and T cells. A mouse mutant (C.B-17scid) with severe combined immune deficiency, i.e., that lacks functional B and T cells, shows no evidence of such gene rearrangements. However, rearrangements were detected in Abelson murine leukemia virus-transformed bone marrow cells and in spontaneous thymic lymphomas from C.B-17scid mice. Most of these rearrangements were abnormal: approximately 80% of Igh rearrangements deleted the entire Jh region, and approximately 60% of TCR beta rearrangements deleted the entire J beta 2 region. The deletions appeared to result from faulty D-to-J recombination. No such abnormal rearrangements were detected in transformed tissues from control mice. The scid mutation may adversely affect the recombinase system catalyzing the assembly of antigen receptor genes in developing B and T lymphocytes. 相似文献
363.
Two furanoditerpenoid lactones, were isolated from the seeds of Caesalpinia minax Hance. Their structures and stereochemistry have been established by spectral data and single crystal X-ray analysis. 相似文献
364.
ERK activation mediates cell cycle arrest and apoptosis after DNA damage independently of p53 总被引:22,自引:0,他引:22
Tang D Wu D Hirao A Lahti JM Liu L Mazza B Kidd VJ Mak TW Ingram AJ 《The Journal of biological chemistry》2002,277(15):12710-12717
In response to DNA damage, ataxia-telangiectasia mutant and ataxia-telangiectasia and Rad-3 activate p53, resulting in either cell cycle arrest or apoptosis. We report here that DNA damage stimuli, including etoposide (ETOP), adriamycin (ADR), ionizing irradiation (IR), and ultraviolet irradiation (UV) activate ERK1/2 (ERK) mitogen-activated protein kinase in primary (MEF and IMR90), immortalized (NIH3T3) and transformed (MCF-7) cells. ERK activation in response to ETOP was abolished in ATM-/- fibroblasts (GM05823) and was independent of p53. The MEK1 inhibitor PD98059 prevented ERK activation but not p53 stabilization. Maximal ERK activation in response to DNA damage was not attenuated in MEF(p53-/-). However, ERK activation contributes to either cell cycle arrest or apoptosis in response to low or high intensity DNA insults, respectively. Inhibition of ERK activation by PD98059 or U0126 attenuated p21(CIP1) induction, resulting in partial release of the G(2)/M cell cycle arrest induced by ETOP. Furthermore, PD98059 or U0126 also strongly attenuated apoptosis induced by high dose ETOP, ADR, or UV. Conversely, enforced activation of ERK by overexpression of MEK-1/Q56P sensitized cells to DNA damage-induced apoptosis. Taken together, these results indicate that DNA damage activates parallel ERK and p53 pathways in an ATM-dependent manner. These pathways might function cooperatively in cell cycle arrest and apoptosis. 相似文献
365.
The C-terminal fragment of the ribosomal P protein complexed to trichosanthin reveals the interaction between the ribosome-inactivating protein and the ribosome
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Priscilla Hiu-Mei Too Meiji Kit-Wan Ma Amanda Nga-Sze Mak Yuen-Ting Wong Christine Kit-Ching Tung Guang Zhu Shannon Wing-Ngor Au Kam-Bo Wong Pang-Chui Shaw 《Nucleic acids research》2009,37(2):602-610
Ribosome-inactivating proteins (RIPs) inhibit protein synthesis by enzymatically depurinating a specific adenine residue at the sarcin-ricin loop of the 28S rRNA, which thereby prevents the binding of elongation factors to the GTPase activation centre of the ribosome. Here, we present the 2.2 Å crystal structure of trichosanthin (TCS) complexed to the peptide SDDDMGFGLFD, which corresponds to the conserved C-terminal elongation factor binding domain of the ribosomal P protein. The N-terminal region of this peptide interacts with Lys173, Arg174 and Lys177 in TCS, while the C-terminal region is inserted into a hydrophobic pocket. The interaction with the P protein contributes to the ribosome-inactivating activity of TCS. This 11-mer C-terminal P peptide can be docked with selected important plant and bacterial RIPs, indicating that a similar interaction may also occur with other RIPs. 相似文献
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368.
Mak MW Guo J Kung SY 《IEEE/ACM transactions on computational biology and bioinformatics / IEEE, ACM》2008,5(3):416-422
The subcellular locations of proteins are important functional annotations. An effective and reliable subcellular localization method is necessary for proteomics research. This paper introduces a new method---PairProSVM---to automatically predict the subcellular locations of proteins. The profiles of all protein sequences in the training set are constructed by PSI-BLAST and the pairwise profile-alignment scores are used to form feature vectors for training a support vector machine (SVM) classifier. It was found that PairProSVM outperforms the methods that are based on sequence alignment and amino-acid compositions even if most of the homologous sequences have been removed. This paper also demonstrates that the performance of PairProSVM is sensitive (and somewhat proportional) to the degree of its kernel matrix meeting the Mercer's condition. PairProSVM was evaluated on Reinhardt and Hubbard's, Huang and Li's, and Gardy et al.'s protein datasets. The overall accuracies on these three datasets reach 99.3\%, 76.5\%, and 91.9\%, respectively, which are higher than or comparable to those obtained by sequence alignment and by the methods compared in this paper. 相似文献
369.
Chaya T L Yuen Dawn G L Thean Becky K C Chan Peng Zhou Cynthia C S Kwok Hoi Yee Chu Maggie S H Cheung Bei Wang Yee Man Chan Silvia Y L Mak Anskar Y Leung Gigi C G Choi Zongli Zheng Alan S L Wong 《Nucleic acids research》2022,50(3):1650
The Cas9 nuclease from Staphylococcus aureus (SaCas9) holds great potential for use in gene therapy, and variants with increased fidelity have been engineered. However, we find that existing variants have not reached the greatest accuracy to discriminate base mismatches and exhibited much reduced activity when their mutations were grafted onto the KKH mutant of SaCas9 for editing an expanded set of DNA targets. We performed structure-guided combinatorial mutagenesis to re-engineer KKH-SaCas9 with enhanced accuracy. We uncover that introducing a Y239H mutation on KKH-SaCas9’s REC domain substantially reduces off-target edits while retaining high on-target activity when added to a set of mutations on REC and RuvC domains that lessen its interactions with the target DNA strand. The Y239H mutation is modelled to have removed an interaction from the REC domain with the guide RNA backbone in the guide RNA-DNA heteroduplex structure. We further confirmed the greatly improved genome-wide editing accuracy and single-base mismatch discrimination of our engineered variants, named KKH-SaCas9-SAV1 and SAV2, in human cells. In addition to generating broadly useful KKH-SaCas9 variants with unprecedented accuracy, our findings demonstrate the feasibility for multi-domain combinatorial mutagenesis on SaCas9’s DNA- and guide RNA- interacting residues to optimize its editing fidelity. 相似文献
370.