Freeze-drying of nanostructure lipid carriers by different carbohydrate polymers used as cryoprotectants

Freeze-drying technique preserves the stability of nanoparticles. The objective of this study was optimization of freeze-drying condition of nano lipid carriers (NLCs). NLCs were prepared by emulsion-solvent evaporation followed by ultra-sonication method. Different carbohydrate and polymeric cryoprotectants including Microcelac^® (mixture of lactose and Avicel), Avicel PH102 (microcrystalline cellulose), mannitol, sucrose, Avicel RC591 (mixture of microcrystalline cellulose and sodium carboxymethyl cellulose), maltodextrine, Aerosil and PEG4000 were tested initially. The NLCs showing lower particle size growth and greater absolute zeta potential after freeze drying were chosen for further investigation using Taguchi optimization method. Studied factors included cryoprotectant type and concentration, freezing temperatures applied at different time periods and sublimation time. Sucrose, Avicel RC591 and Aerosil were selected as cryoprotectants from initial screening tests. Increasing their concentration increased the particle size. 1% of Avicel RC591, 24 h of freezing at ?70 °C and 48 h sublimation time showed lower growth in particle size.     

Exogenous Methyl Jasmonate Treatment Induces Defense Response Against Fusarium culmorum in Wheat Seedlings

Journal of Plant Growth Regulation - The Fusarium crown and root rot (FCRR) pathogen Fusarium culmorum (Fc) is a hemibiotrophic pathogen, with a short biotrophic phase preceding necrotropism....     

Spectroscopic,electrochemical, docking and molecular dynamics studies on the interaction of three oxovanadium (IV) Schiff base complexes with bovine serum albumin and their cytotoxicity against cancer

This study was designed to investigate the interaction of three oxovanadium (IV) Schiff base complexes with bovine serum albumin (BSA) by means of various spectroscopic and electrochemical methods along with molecular docking study and molecular dynamics simulations. Binding constants were estimated by fluorescence and UV-Vis spectroscopy. The results indicated a good affinity of the complexes for BSA in which furyl derivative had more activity. Molecular docking study showed that these complexes have the similar binding modes and located within subdomain IB in site III of BSA. The supporting of molecular docking and molecular dynamics results by experimental data, confirms the validity of the interactions data obtained by these methods. Biological activity against cancer cell showed that furyl derivative has higher activity than other complexes. Pharmaceutical analysis also showed that, these complexes potentially can be used as anticancer agents.