全文获取类型
收费全文 | 1627篇 |
免费 | 191篇 |
出版年
2022年 | 8篇 |
2021年 | 31篇 |
2020年 | 14篇 |
2019年 | 14篇 |
2018年 | 15篇 |
2017年 | 9篇 |
2016年 | 19篇 |
2015年 | 66篇 |
2014年 | 53篇 |
2013年 | 84篇 |
2012年 | 86篇 |
2011年 | 93篇 |
2010年 | 44篇 |
2009年 | 47篇 |
2008年 | 87篇 |
2007年 | 80篇 |
2006年 | 85篇 |
2005年 | 71篇 |
2004年 | 60篇 |
2003年 | 74篇 |
2002年 | 61篇 |
2001年 | 61篇 |
2000年 | 64篇 |
1999年 | 55篇 |
1998年 | 29篇 |
1997年 | 23篇 |
1996年 | 21篇 |
1995年 | 22篇 |
1994年 | 28篇 |
1993年 | 25篇 |
1992年 | 27篇 |
1991年 | 22篇 |
1990年 | 33篇 |
1989年 | 28篇 |
1988年 | 28篇 |
1987年 | 18篇 |
1986年 | 16篇 |
1985年 | 19篇 |
1984年 | 19篇 |
1983年 | 15篇 |
1982年 | 9篇 |
1981年 | 12篇 |
1979年 | 20篇 |
1978年 | 15篇 |
1977年 | 14篇 |
1976年 | 10篇 |
1975年 | 8篇 |
1974年 | 8篇 |
1973年 | 12篇 |
1972年 | 8篇 |
排序方式: 共有1818条查询结果,搜索用时 15 毫秒
91.
92.
Martin Frommhold Arend Heim Mikhail Barabanov Franziska Maier Ralf‐Udo Mühle Sergei M. Smirenski Wieland Heim 《Ecology and evolution》2019,9(24):14430-14441
The selection of a nest site is crucial for successful reproduction of birds. Animals which re‐use or occupy nest sites constructed by other species often have limited choice. Little is known about the criteria of nest‐stealing species to choose suitable nesting sites and habitats. Here, we analyze breeding‐site selection of an obligatory “nest‐cleptoparasite”, the Amur Falcon Falco amurensis. We collected data on nest sites at Muraviovka Park in the Russian Far East, where the species breeds exclusively in nests of the Eurasian Magpie Pica pica. We sampled 117 Eurasian Magpie nests, 38 of which were occupied by Amur Falcons. Nest‐specific variables were assessed, and a recently developed habitat classification map was used to derive landscape metrics. We found that Amur Falcons chose a wide range of nesting sites, but significantly preferred nests with a domed roof. Breeding pairs of Eurasian Hobby Falco subbuteo and Eurasian Magpie were often found to breed near the nest in about the same distance as neighboring Amur Falcon pairs. Additionally, the occurrence of the species was positively associated with bare soil cover, forest cover, and shrub patches within their home range and negatively with the distance to wetlands. Areas of wetlands and fallow land might be used for foraging since Amur Falcons mostly depend on an insect diet. Additionally, we found that rarely burned habitats were preferred. Overall, the effect of landscape variables on the choice of actual nest sites appeared to be rather small. We used different classification methods to predict the probability of occurrence, of which the Random forest method showed the highest accuracy. The areas determined as suitable habitat showed a high concordance with the actual nest locations. We conclude that Amur Falcons prefer to occupy newly built (domed) nests to ensure high nest quality, as well as nests surrounded by available feeding habitats. 相似文献
93.
Roberto Menzel Samuel Dorey Tanja Maier Ina Pahl Armin Hauk 《Biotechnology progress》2022,38(1):e3214
The biopharmaceutical industry gains enormous flexibility in production processes by using sterilized preassembled single-use devices. Gamma irradiation is an established sterilization technology that may be restricted in the future by the availability of 60Co as irradiation source and irradiation capacities. X-ray technology is considered an alternative type of radiation for sterilizing SU equipment. In the context of extractables and leachables—one concern connected with the use of single-use process equipment—the effect of X-ray irradiation on the extractables profile of the materials needs to be compared to established gamma irradiation to qualify this alternative technology. An approach is presented to obtain robust and comprehensive extractables data for materials used in SU devices after sterilization either using X-ray or gamma irradiation. A careful selection of the test items and the test design allows a one-to-one comparison of data obtained from a combination of orthogonal analytical techniques. The extractables of a modern SU film material and the copolyester Tritan™ are evaluated. The data presented allow a risk evaluation on the safety of this new sterilization modality for biopharmaceutical applications. It is demonstrated that the extractables profile of a polymer is not affected by the type of irradiation used for sterilization. 相似文献
94.
95.
Hideyuki Yoshida Caleb A. Lareau Ricardo N. Ramirez Samuel A. Rose Barbara Maier Aleksandra Wroblewska Fiona Desland Aleksey Chudnovskiy Arthur Mortha Claudia Dominguez Julie Tellier Edy Kim Dan Dwyer Susan Shinton Tsukasa Nabekura YiLin Qi Bingfei Yu Michelle Robinette Christophe Benoist 《Cell》2019,176(4):897-912.e20
96.
Na Wang Sari Palmroth Christopher A. Maier Jean‐Christophe Domec Ram Oren 《Plant, cell & environment》2019,42(5):1690-1704
The genus Pinus has wide geographical range and includes species that are the most economically valued among forest trees worldwide. Pine needle length varies greatly among species, but the effects of needle length on anatomy, function, and coordination and trade‐offs among traits are poorly understood. We examined variation in leaf morphological, anatomical, mechanical, chemical, and physiological characteristics among five southern pine species: Pinus echinata, Pinus elliottii, Pinus palustris, Pinus taeda, and Pinus virginiana. We found that increasing needle length contributed to a trade‐off between the relative fractions of support versus photosynthetic tissue (mesophyll) across species. From the shortest (7 cm) to the longest (36 cm) needles, mechanical tissue fraction increased by 50%, whereas needle dry density decreased by 21%, revealing multiple adjustments to a greater need for mechanical support in longer needles. We also found a fourfold increase in leaf hydraulic conductance over the range of needle length across species, associated with weaker upward trends in stomatal conductance and photosynthetic capacity. Our results suggest that the leaf size strongly influences their anatomical traits, which, in turn, are reflected in leaf mechanical support and physiological capacity. 相似文献
97.
Feeding experiments were conducted in situ in flow through boxes to determine the vulnerability of different prey types (the mayfly larvae Baetis, Ephemerella and Ecdyonurus spp., the caddisfly larva Hydropsyche spp. and the crustacean Gammarus spp.) to the predaceous water bug Aphelocheirus aestivalis and to estimate the predation impact of Aphelocheirus on prey populations. Experiments with equal densities of mixed prey and experiments where each prey was tested individually revealed that Baetis and Ephemerella were most vulnerable to Aphelocheirus predation: Hydropsyche, Ecdyonurus and Gammarus were little/not preyed upon. The present results suggest that vulnerability of prey depends mainly upon prey mobility and microhabitat overlap between predator and prey and that feeding behaviour of Aphelocheirus resembles more that of megalopterans than of stoneflies. Experiments with different prey densities (120–720 prey m−2) showed that the predation rate of Aphelocheirus increased with increasing prey density. Experiments with different substrates documented that mortality rates of prey decreased with increasing substrate complexity. When substrate conditions were complex mortality rates in the control and in the experimental boxes were the same which suggest little importance of Aphelocheirus predation on mayflies in the study site. 相似文献
98.
Bingchuan Wei Nicholas Woon Lu Dai Raphael Fish Michelle Tai Winode Handagama Ashley Yin Jia Sun Andrew Maier Dana McDaniel Elvira Kadaub Jessica Yang Miguel Saggu Ann Woys Oxana Pester Danny Lambert Alex Pell Zhiqi Hao Gordon Magill Jack Yim Jefferson Chan Lindsay Yang Frank Macchi Christian Bell Galahad Deperalta Yan Chen 《MABS-AUSTIN》2022,14(1)
99.
M.J. Bogusz R.-D. Maier K.-H. Schiwy-Bochat U. Kohls 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1996,683(2):177
Four commercially available types of mixed-phase solid-phase extraction (SPE) columns (Bond Elut Certify, Isolute Confirm HCX, Chromabond Drug and Bakerbond Narc-2) were examined in order to compare the extraction efficiencies and chromatographic purity of extracts. The absolute recovery of morphine, 6-monoacetylmorphine and codeine was examined in blood and serum (ten samples each at two concentration levels), using SPE columns of the same batch. GC-MS (ion trap) and HPLC with amperometric detection were used for quantitation. A distinct variability in extraction recovery was observed among the same batches of all brands of SPE columns. All extracts were chromatographically pure and no interfering peaks were observed, neither in GC-MS nor in HPLC examinations, but in some extracts large peaks of plasticizers were identified. The measurements of flow velocities of the same samples of blood or serum through the SPE columns of the same batch showed very large variability of random charactere. The morphometric analysis of particles was performed for two batches of each sort of SPE columns by means of an image analysing system. Symmetrical distribution of particle size was observed only in Chromabond MN Drug packing, while in other cartridges large fractions of fine particles and nonhomogenous distribution were found. Only in one case the morphometric findings were pretty concordant with the data available from the manufacturer; in two cases, observed data varied considerably from the expected, and in one case no information was available at all. The study showed generally that there was room for improvement in the quality of mixed-phase SPE columns. 相似文献
100.
Hairless is a member of the Notch signalling pathway, where it acts as antagonist by binding to Suppressor of Hairless [Su(H)], thereby
inhibiting Notch target gene activation. The pathway and its members are highly conserved in metazoans from worms to humans.
However, a Hairless orthologue from another species has not yet been identified. The identification of Hairless in largely diverged species by cross-hybridization has failed so far probably due to a low degree of conservation. Therefore,
we turned to D. hydei where a Hairless mutation has been described before. The D. hydei Hairless orthologue is reasonably well conserved with regard to gene structure and expression. The prospective Hairless protein orthologues
share several highly conserved regions which are separated by quite diverged stretches. As to be expected, the largest region
of high conservation corresponds to the Su(H) binding domain. This region is also functionally conserved, since this D. hydei protein domain binds very strongly to the D. melanogaster Su(H) protein. The other conserved regions support our earlier structure-function analysis since they nicely correspond to
previously defined, functionally important protein domains. Most notably, the very C-terminal domain which is very sensitive
to structural alterations, is nearly identical between the two species. In summary, this evolutionary study improves the knowledge
on functionally significant domains of the Hairless protein, and may be helpful for the future identification of homologues
in other animals, especially in vertebrates.
Received: 26 August 1998 / Accepted: 9 November 1998 相似文献