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921.
James Hury Uma Nagaswamy Maia Larios-Sanz George E. Fox 《Origins of life and evolution of the biosphere》2006,36(4):421-429
The modern ribosome and its component RNAs are quite large and it is likely that at an earlier time they were much smaller. Hence, not all regions of the modern ribosomal RNAs (rRNA) are likely to be equally old. In the work described here, it is hypothesized that the oldest regions of the RNAs will usually be highly integrated into the machinery. When this is the case, an examination of the interconnectivity between local RNA regions can provide insight to the relative age of the various regions. Herein, we describe an analysis of all known long-range RNA/RNA interactions within the 23S rRNA and between the 23S rRNA and the 16S rRNA in order to assess the interconnectivity between the usual Domains as defined by secondary structure. Domain V, which contains the peptidyl transferase center is centrally located, extensively connected, and therefore likely to be the oldest region. Domain IV and Domain II are extensively interconnected with both themselves and Domain V. A portion of Domain IV is also extensively connected with the 30S subunit and hence Domain IV may be older than Domain II. These results are consistent with other evidence relating to the relative age of RNA regions. Although the relative time of addition of the GTPase center can not be reliably deduced it is pointed out that the development of this may have dramatically affected the progenotes that preceded the last common ancestor. 相似文献
922.
Paulo Milet-Pinheiro Arthur Domingos-Melo João B. Olivera Nayara S.L. Albuquerque Ana Carolina G. Costa Sinzinando Albuquerque-Lima Marcelo F.R. Silva Daniela M.A.F. Navarro Artur C.D. Maia Lise-Lotte Gundersen Mario Schubert Stefan Dötterl Isabel C. Machado 《Current biology : CB》2021,31(4):860-868.e4
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923.
Raquel de Oliveira Lopes Patrícia Maia Pereira Aline Ramalho Brandão Pereira Keysson Vieira Fernandes Julia Finamor Carvalho Alexandre da Silva de França 《Biocatalysis and Biotransformation》2020,38(6):415-430
AbstractAtrazine is the most common herbicide applied in crops of economic relevance, such as sugar cane, soybean, and corn. Atrazine and its derivatives desethylatrazine (DEA) and desisopropylatrazine (DIA) are toxic to the environment, affecting animal and human health. Thus, this study aimed to evaluate the degradation of atrazine and its derivatives by the fungus Pleurotus ostreatus INCQS 40310, as well as the potential of the enzymes involved in this process. P. ostreatus INCQS 40310 was able to degrade atrazine (82%), DEA (71%), and DIA (56%) over 22?days of fungal cultivation. Proteomic analysis indicated the participation of hydrolases and peroxidases during the degradation process. Additionally, resting cells of the fungus were tested to verify the action of intracellular enzymes in the degradation process, suggesting the participation of cytochrome P450 enzymatic complex. Resting cells experiments promoted the degradation of 50% of atrazine, 36% of DIA, 30% of DEA. So far, this is the first work evaluating the biodegradation of DEA and DIA by fungus. 相似文献