全文获取类型
收费全文 | 854篇 |
免费 | 69篇 |
专业分类
923篇 |
出版年
2023年 | 5篇 |
2022年 | 15篇 |
2021年 | 21篇 |
2020年 | 22篇 |
2019年 | 23篇 |
2018年 | 33篇 |
2017年 | 42篇 |
2016年 | 47篇 |
2015年 | 56篇 |
2014年 | 58篇 |
2013年 | 57篇 |
2012年 | 59篇 |
2011年 | 59篇 |
2010年 | 38篇 |
2009年 | 33篇 |
2008年 | 41篇 |
2007年 | 48篇 |
2006年 | 37篇 |
2005年 | 34篇 |
2004年 | 30篇 |
2003年 | 23篇 |
2002年 | 22篇 |
2001年 | 8篇 |
2000年 | 9篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 5篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 5篇 |
1987年 | 3篇 |
1986年 | 5篇 |
1985年 | 3篇 |
1984年 | 6篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 5篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1978年 | 3篇 |
1976年 | 11篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1972年 | 2篇 |
1957年 | 1篇 |
排序方式: 共有923条查询结果,搜索用时 0 毫秒
81.
The prevalence of Hepatozoon parasites in 460 lizards from North Africa was studied by amplification and sequencing of the 18S rRNA gene. The phylogenetic analysis of the 18S rRNA gene provides new insights into the phylogeny of these parasites with multiple genetically distinct lineages recovered. Parasite prevalence differed significantly between lacertid lizards and geckos. Our results show that there is limited host specificity and no clear relation to the geographical distribution of Hepatozoon parasites. 相似文献
82.
83.
Régis Augusto Pescinelli João Alberto Farinelli Pantaleão Thiago Maia Davanso 《Invertebrate reproduction & development.》2014,58(2):108-114
An analysis of relative growth was performed to determine the size at morphological sexual maturity of Trichodactylus fluviatilis Latreille 1828. Samples were collected by trapping at night from January 2010 through June 2011 from the Barreiro River (22°34′42″S; 49°11′92″W), west central São Paulo State, Brazil. The following measurements were obtained (mm): carapace width (CW) (independent variable), cheliped propodus length (PL), cheliped propodus height (PH), cheliped propodus width (PW), first pleopod length, and abdomen width (AW). The males showed positive allometry in the juvenile and adult stages for the following relationships: PL vs. CW, PH vs. CW, and PW vs. CW. In the females, a positive allometry was observed for AW vs. CW in the juvenile stage, and a negative allometry was observed for this relationship in the adult stage. The differential growth of the chelipeds in males could be related to territorial conflict and courtship, whereas the changes in the growth of the female abdomen most likely favor higher reproductive success. 相似文献
84.
Gisela H. Maia José L. Quesado Joana I. Soares Joana M. do Carmo Pedro A. Andrade José P. Andrade Nikolai V. Lukoyanov 《PloS one》2014,9(1)
Treating rats with kainic acid induces status epilepticus (SE) and leads to the development of behavioral deficits and spontaneous recurrent seizures later in life. However, in a subset of rats, kainic acid treatment does not induce overt behaviorally obvious acute SE. The goal of this study was to compare the neuroanatomical and behavioral changes induced by kainate in rats that developed convulsive SE to those who did not. Adult male Wistar rats were treated with kainic acid and tested behaviorally 5 months later. Rats that had experienced convulsive SE showed impaired performance on the spatial water maze and passive avoidance tasks, and on the context and tone retention tests following fear conditioning. In addition, they exhibited less anxiety-like behaviors than controls on the open-field and elevated plus-maze tests. Histologically, convulsive SE was associated with marked neuron loss in the hippocampal CA3 and CA1 fields, and in the dentate hilus. Rats that had not experienced convulsive SE after kainate treatment showed less severe, but significant impairments on the spatial water maze and passive avoidance tasks. These rats had fewer neurons than control rats in the dentate hilus, but not in the hippocampal CA3 and CA1 fields. Correlational analyses revealed significant relationships between spatial memory indices of rats and neuronal numbers in the dentate hilus and CA3 pyramidal field. These results show that a part of the animals that do not display intense behavioral seizures (convulsive SE) immediately after an epileptogenic treatment, later in life, they may still have noticeable structural and functional changes in the brain. 相似文献
85.
Juliana Pereira Clemens Schlindwein Yasmine Antonini Artur Campos Dália Maia Stefan Dötterl Cristiane Martins Daniela Maria do Amaral Ferraz Navarro Reisla Oliveira 《Biological journal of the Linnean Society. Linnean Society of London》2014,111(3):679-691
Cyclocephline scarabs and their host plants are documented as highly specialized plant–pollinator associations, with various fine‐tuned adaptations. We studied the association between Philodendron adamantinum, a species endemic to the Espinhaço Range in Minas Gerais, South‐East Brazil, and its exclusive pollinators. We focused on the pollination mechanism and reproductive success of P. adamantinum, analysed its floral scent composition, and performed field bioassays to verify the scent‐mediated attraction of pollinators. The reproductive success of P. adamantinum depends on the presence of Erioscelis emarginata (Scarabaeidae, Cyclocephalini), its sole pollinator. At dusk, the inflorescences heat up to 18 °C above the surrounding ambient air temperature and give off a strong sweet odour, from which 32 volatile compounds were isolated. Dihydro‐β‐ionone, the major constituent in the floral scent bouquet, lures individuals of E. emarginata when applied to scented artificial decoys, either alone or blended with methyl jasmonate. We attribute the low fruit set of P. adamantinum at our study sites to pollinator limitation of small and isolated populations and geitonogamic pollen flow of vegetatively generated clonal plant groups. The interaction between P. adamantinum and E. emarginata shows common traits typical of the known plant–pollinator associations involving cyclocephaline scarabs: the asymmetrical dependence of plants on their pollinators, and the scent‐mediated interaction between flowers and beetles. In addition to updating the current catalogue of active compounds of cantharophilous pollination systems, further experimental studies should elucidate the role of the specific chemical compounds that attract pollinators along different time and biogeographic scales. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 111 , 679–691. 相似文献
86.
Relationship between lifestyle behaviors and obesity in children ages 9–11: Results from a 12‐country study 下载免费PDF全文
Peter T. Katzmarzyk Tiago V. Barreira Stephanie T. Broyles Catherine M. Champagne Jean‐Philippe Chaput Mikael Fogelholm Gang Hu William D. Johnson Rebecca Kuriyan Anura Kurpad Estelle V. Lambert Carol Maher Jose Maia Victor Matsudo Timothy Olds Vincent Onywera Olga L. Sarmiento Martyn Standage Mark S. Tremblay Catrine Tudor‐Locke Pei Zhao Timothy S. Church for the ISCOLE Research Group 《Obesity (Silver Spring, Md.)》2015,23(8):1696-1702
87.
Paulo Marcos da Matta Guedes Fredy R. S. Gutierrez Flavia L. Maia Cristiane M. Milanezi Grace K. Silva Wander R. Pavanelli Jo?o S. Silva 《PLoS neglected tropical diseases》2010,4(2)
Background
Chagas disease is a neglected disease caused by the intracellular parasite Trypanosoma cruzi. Around 30% of the infected patients develop chronic cardiomyopathy or megasyndromes, which are high-cost morbid conditions. Immune response against myocardial self-antigens and exacerbated Th1 cytokine production has been associated with the pathogenesis of the disease. As IL-17 is involved in the pathogenesis of several autoimmune, inflammatory and infectious diseases, we investigated its role during the infection with T. cruzi.Methodology/Principal Findings
First, we detected significant amounts of CD4, CD8 and NK cells producing IL-17 after incubating live parasites with spleen cells from normal BALB/c mice. IL-17 is also produced in vivo by CD4+, CD8+ and NK cells from BALB/c mice on the early acute phase of infection. Treatment of infected mice with anti-mouse IL-17 mAb resulted in increased myocarditis, premature mortality, and decreased parasite load in the heart. IL-17 neutralization resulted in increased production of IL-12, IFN-γ and TNF-α and enhanced specific type 1 chemokine and chemokine receptors expression. Moreover, the results showed that IL-17 regulates T-bet, RORγt and STAT-3 expression in the heart, showing that IL-17 controls the differentiation of Th1 cells in infected mice.Conclusion/Significance
These results show that IL-17 controls the resistance to T. cruzi infection in mice regulating the Th1 cells differentiation, cytokine and chemokine production and control parasite-induced myocarditis, regulating the influx of inflammatory cells to the heart tissue. Correlations between the levels of IL-17, the extent of myocardial destruction, and the evolution of cardiac disease could identify a clinical marker of disease progression and may help in the design of alternative therapies for the control of chronic morbidity of chagasic patients. 相似文献88.
da Cunha EF de Castro Ramalho T Bicca de Alencastro R Maia ER 《Journal of biomolecular structure & dynamics》2004,22(2):119-130
There are major differences between the structures of human dihydrofolate reductase (hDHFR) and Mycobacterium tuberculosis dihydrofolate reductase (mtDHFR). These differences may allow us to design more selective mtDHFR inhibitors. In this paper we study the reactions of six different compounds derived from 5-deazapteridine with human and bacterial enzymes. Results suggest that the addition of hydrophobic groups to the aminophenyl ring would increase mtDHFR-inhibitor affinity and selectivity. 相似文献
89.
90.
J. M. Maia C. E. Costa de Macedo E. L. Voigt J. B. S. Freitas J. A. G. Silveira 《Biologia Plantarum》2010,54(1):159-163
The aim of this work was to investigate the role of the antioxidant enzymes in salt tolerance comparing the salt-sensitive
(Pérola) and a salt-tolerant (Pitiúba) cultivar of cowpea [Vigna unguiculata (L.) Walp.]. Salt stress (100 mM NaCl for 8 d) reduced the leaf growth rate more in the sensitive cultivar. The salt-induced
decrease in the relative water content, Na+ accumulation and increase in leaf electrolyte leakage was similar in both cultivars. Salt stress induced a higher increase
in the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and phenol peroxidase (POX) in the tolerant cultivar
than in sensitive one. 相似文献