排序方式: 共有39条查询结果,搜索用时 46 毫秒
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Elham Roshandel Sayeh Parkhideh Haniyeh Ghaffari Nazari Mahshid Mehdizadeh Hossein Bonakchi Ghazaleh Sankanian Abbas Hajifathali 《Reports of Biochemistry & Molecular Biology》2021,10(2):204
Background:The discovery of biomarkers to predict the development of complications associated with hematopoietic stem cell transplantation (HSCT) offers a potential avenue for the early identification and treatment of these life-threatening consequences. Serum lactate dehydrogenase (sLDH) has been identified as a potential biomarker for determining the outcome of allogenic HSCT (allo-HSCT).Methods:A retrospective study was performed using data collected from 204 allo-HSCT recipient patients to examine the predictive value of sLDH levels pre- and post-allo-HSCT on patient survival, graft-versus-host-disease (GVHD) incidence, and time to platelet/white blood cells (WBC) engraftment.Results:Our findings show that neither pre- (p= 0.61) nor post-transplantation (p= 0.55) sLDH levels were associated with GVHD incidence. However, elevated sLDH levels pre- and post-transplantation (≥ 386 and ≥ 409 IU/mL, respectively) were found to be adverse risk factors for patient survival (p= 0.16, p= 0.20, respectively). Furthermore, a median sLDH level ≥ 400 IU/mL from day +5 to day +15 post-transplantation had a significant positive association with enhanced time to platelet and white blood cell (WBC) engraftment, compared to patients with sLDH levels < 400 IU/mL (p< 0.001).Conclusion:Our data suggests that high sLDH levels pre- and post-allo-HSCT could be considered a predictor of poor patient survival. Furthermore, high levels of sLDH days 5-15 post-allo-HSCT could be associated with improved time to platelet and WBC engraftment; however, this appears to come at the cost of increased mortality risk.Key Words: Engraftment, Graft versus host disease, Hematopoietic stem cell transplantation, Lactate dehydrogenase 相似文献
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Hamedi-Asl P Halabian R Bahmani P Mohammadipour M Mohammadzadeh M Roushandeh AM Jahanian-Najafabadi A Kuwahara Y Roudkenar MH 《Cell stress & chaperones》2012,17(2):181-190
The capacity of mesenchymal stem cells (MSCs) to survive and engraft in the target tissue may lead to promising therapeutic
effects. However, the fact that the majority of MSCs die during the first few days following transplantation complicates cell
therapy. Hence, it is necessary to strengthen the stem cells to withstand the rigors of the microenvironment to improve the
efficacy of cell therapy. In this study, we manipulated MSCs to express a cytoprotective factor, heme oxygenase-1 (HO-1),
to address this issue. Full-length cDNA of human HO-1 was isolated and cloned into TOPO vector by TOPO cloning reaction. Then,
the construct was ligated to gateway adapted adenovirus expression vector by LR recombination reaction. Afterwards, the recombinant
virus expressing HO-1 was produced in appropriate mammalian cell line and used to infect MSCs. The HO-1 engineered MSCs were
exposed to hypoxic and oxidative stress conditions followed by evaluation of the cells’ viability and apoptosis. Transient
expression of HO-1 was detected within MSCs. It was observed that HO-1 expression could protect MSCs against cell death and
the apoptosis triggered by hypoxic and oxidative stress conditions. The MSCs-HO-1 retained their ability to differentiate
into adipogenic, chondrogenic, or osteogenic lineages. These findings could be applied as a strategy for prevention of graft
cell death in MSCs-based cell therapy and is a good demonstration of how an understanding of cellular stress responses can
be used for practical applications. 相似文献
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Safaralizadeh R Sirjani M Pourpak Z Kardar G Teimourian S Shams S Namdar Z Kazemnejad A Moin M 《BioFactors (Oxford, England)》2007,31(2):127-131
INTRODUCTION: Selenium is one of the essential trace elements which is more notified in children in recent years. Reliable age-specific reference values for selenium in children in Iran are not clear and are important for the identification of selenium deficiency and some other researches that is the aim of this study. METHODS: Serum samples of 216 healthy children were analyzed by using hydride generation and flame atomic absorption spectroscopy to measured selenium level. RESULTS: The mean and standard deviation of serum selenium level in children 0-16 years old (mean: 39.83 months) was 72.14+/-16.80 microg/l. There was significant difference in serum selenium concentration between two sexes which was 76.78+/-15.24 microg/l and 69.56+/-17.09 microg/l in girls and boys respectively (P value=0.002). There was also a positive correlation between higher selenium serum concentration and age in both sexes. CONCLUSION: Essential trace element's normal ranges are fundamental data which could use in many studies. Serum selenium concentration in healthy Iranian children that found in this study is very close to serum concentration of European children. Our findings may reveal nutritional culture's similarity. 相似文献
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Zarrin A Foroozesh M Hamidi M Mohammadi-Samani S 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2006,833(2):199-203
Development of reliable assay methods for quantitation of interferons in dosage forms has encountered serious limitations because of the physicochemical nature of these proteins as well as the sensitivity/selectivity issues. A rapid, available, and easy-to-use reversed-phase HPLC method has been developed for quantitative analysis of interferon-alpha2b in pharmaceuticals. The reversed- phase method was based on a gradient system using a wide-pore C4 column and produced linear response in drug concentration range of 0.25-5 MIU (r = 0.9997). The average within-run and between-run variables of the method were 4.19 and 9.40%, respectively, with corresponding average accuracies of 99.48+/-4.11 and 102.83+/-9.51%. The limits of detection (LOD) and quantitation (LOQ) of the method were 0.125 and 0.25MIU/ml, respectively. The practical applicability of the method was proven throughout a post-marketing quality control program on interferon-alpha2b products (PD-feron) produced and marketed in Iran. 相似文献
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Ebrahimnejad Mahshid Azizi Paniz Alipour Vahide Zarrindast Mohammad-Reza Vaseghi Salar 《Neurochemical research》2022,47(6):1477-1490
Neurochemical Research - Evidence has shown the beneficial effects of exercise on learning and memory. However, many studies have reported controversial results, indicating that exercise can impair... 相似文献