排序方式: 共有242条查询结果,搜索用时 15 毫秒
91.
Roberto Carlos Agís‐Balboa Cemil Kerimoglu Eva Benito Michael Gertig Sanaz Bahari‐Javan Gaurav Jain Susanne Burkhardt Ivana Delalle Alexander Jatzko Markus Dettenhofer Patricia A Zunszain Andrea Schmitt Peter Falkai Julius C Pape Elisabeth B Binder Christophe Mulle Andre Fischer Farahnaz Sananbenesi 《The EMBO journal》2017,36(19):2815-2828
92.
Maryam Hassantash Hedayat Sahraei Zahra Bahari Gholam Hossein Meftahi Roshanak Vesali 《生物学前沿》2017,12(4):298-310
Objective
The D2 dopamine receptor is found in different parts of the amygdala. However, its contribution to stress is unknown. Thus, in the present study, we examined the effects of excitation and inhibition of D2 dopamine receptors in the amygdala on the metabolic and hormonal changes in response to stress.Methods
Bilateral amygdala cannulation was carried out in Swiss-Webster mice (n = 7). On recovery, different doses of the dopamine D2 receptor antagonist, sulpiride (1, 5 and 10 μg/mouse) or the dopamine D2 receptor agonist, bromocriptine (1, 5 and 10 μg/mouse) were injected into the amygdala. The animals were then placed in stress apparatus (communication box) where they received an electric shock (10 mV voltage, 10 Hz frequency and 60 s duration) after 30 min. The animal's activities were recorded for 10 min before and 10 min after the stress induction. Locomotion, rearing and freezing were investigated. Metabolic changes, such as food and water intake and anorexia, were studied.Results
The results show that stress increased the concentration of plasma corticosterone, which was followed by a decrease in locomotion and rearing and an increase in freezing behavior. Furthermore, both weight and water and food intake were reduced. Administration of bromocriptine led to a reduction of corticosterone at doses of 1 and 5 μg/mouse and an increase of corticosterone at 10 μg/mouse. Additionally, lower doses of bromocriptine (1 and 5 μg/mouse) caused an increase in locomotion and rearing and a decrease in freezing behavior. Similar results were observed with sulpiride injection.Conclusion
D2 dopamine receptors can play a major role in the amygdala in stress. Both an agonist and an antagonist of the D2 receptor attenuate the metabolic and hormonal changes observed in response to stress93.
94.
Soleimanjahi H Roostaee MH Rasaee MJ Mahboudi F Kazemnejad A Bamdad T Zandi K 《FEMS immunology and medical microbiology》2006,46(1):100-106
Herpes simplex virus produces primary and latent infections with periodic recurrency. The prime-boost immunization strategies were studied using a DNA vaccine carrying the full-length glycoprotein D-1 gene and a baculovirus-derived recombinant glycoprotein D, both expressing herpes simplex virus glycoprotein D-1 protein. Immunization with recombinant DNAs encoding antigenic proteins could induce cellular and humoral responses by providing antigen expression in vivo. Higher immune response, however, occurred when the recombinant proteins followed DNA inoculation. While all groups of the immunized mice and positive control group could resist virus challenge, a higher virus neutralizing antibody level was detected in the animals receiving recombinant protein following DNA vaccination. 相似文献
95.
Ali Mahdi Behrad Darvishi Keivan Majidzadeh-A Malihe Salehi Leila Farahmand 《Journal of cellular physiology》2019,234(5):5655-5663
It is now fully recognized that along with multiple physiological functions, angiogenesis is also involved in the fundamental process and pathobiology of several disorders including cancer. Recent studies have fully established the role of angiogenesis in cancer progression as well as invasion and metastasis. Consequently, many therapeutic agents such as monoclonal antibodies targeting angiogenesis pathway have been introduced in clinic with the hope for improving the outcomes of cancer therapy. Bevacizumab (Avastin®) was the first anti-vascular endothelial growth factor (VEGF) targeting monoclonal antibody developed with this purpose and soon received its accelerated US Food and Drug Administration (FDA) approval for treatment of patients with metastatic breast cancer in 2008. However, the failure to meet expecting results in different follow-up studies, forced FDA to remove bevacizumab approval for metastatic breast cancer. Investigations have now revealed that while suppressing VEGF pathway initially decreases tumor progression rate and vasculature density, activation of several interrelated pathways and signaling molecules following VEGF blockade compensate the insufficiency of VEGF and initially blocked angiogenesis, explaining in part the failure observed with bevacizumab single therapy. In present review, we introduce some of the main pathways and signaling molecules involved in angiogenesis and then propose how their interconnection may result in development of resistance to bevacizumab. 相似文献
96.
Simzar Hosseinzadeh Hojjatollah Nazari Negar Sadegzadeh Ali Babaie Mahboubeh Kabiri Noshin Tasharrofi Mahsa Soufi Zomorrod Masoud Soleimani 《Journal of cellular biochemistry》2019,120(2):1511-1521
Among different tissues, endothelial/cardiac types require specific factors to promote myocardial regeneration after occurred injuries. Herein, cardiac stem cells (CSCs) as the major cell population that involved in cardiovascular repair were selected to study the role of polyethyleneimine (PEI) agent on endothelial differentiation. After preparation of electrospun network of PEI with polyacrylonitrile, the related characterizations were carried out including scanning electron microscope (SEM), field-emission SEM, water contact angle, Fourier transform infrared spectroscopy and mechanical properties. Also, the release kinetic of the corresponding agent was studied up to 7 days. The cell differentiation studies were done in the following with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, Real-time polymerase chain reaction and immunostaining method. The whole obtained results approved the higher differentiation of CSCs into endothelial/cardiac cells. Finally, it is recommended that the PEI delivering increases the healing potency of CSCs and accordingly the regeneration speed of damaged cardiovascular tissue would be improved. 相似文献
97.
Aynaz Mihanfar Aydin Raei Sadigh Amir Fattahi Zeinab Latifi Mahsa Hasanzadeh-Moghadam Mahrokh Samadi Laya Farzadi Kobra Hamdi Aliyeh Ghasemzadeh Hamid Reza Nejabati Mohammad Nouri 《Journal of cellular biochemistry》2019,120(9):14274-14284
As a critical stage of pregnancy, the implantation of blastocysts into the endometrium is a progressive, excessively regulated local tissue remodeling step involving a complex sequence of genetic and cellular interplay executed within an optimal time frame. For better understanding the causes of infertility and, more importantly, for developing powerful strategies for successful implantations and combating infertility, an increasing number of recent studies have been focused on the identification and study of newly described substances in the reproductive tree. The endothelins (ET), a 21-aminoacidic family of genes, have been reported to be responsible for the contraction of vascular and nonvascular smooth muscles, including the smooth muscles of the uterus. Therefore, this review aims to comprehensively discuss the physiological role of endothelins and signaling through their receptors, as well as their probable involvement in the implantation process. 相似文献
98.
Zohreh Saltanatpour Behrooz Johari Akram Alizadeh Majid Lotfinia Keivan Majidzadeh-A Behrooz Nikbin Mehdi Kadivar 《Journal of cellular physiology》2019,234(12):22935-22946
A better understanding of cancer stem cells (CSCs) may facilitate the prevention and treatment of cancers. Epithelial-mesenchymal transition (EMT) is a process activated during invasion and metastasis of tumors. EMT induction in normal and tumor cells makes them more resistant to chemotherapy. E-cadherin is a membrane protein and plays a role in tumor invasion, metastasis, and prognosis. Downregulation of E-cadherin is a hallmark of EMT. Here, we created a model of cancer stem-like cells enrichment via EMT induction using E-cadherin downregulation in HT29 cell line using a lentiviral vector carrying shRNA. We aimed to evaluate cancer and anti-CSC chemotherapeutics screening. The markers of EMT and CSCs were assessed and compared with control cells using flow cytometry, real-time PCR, immunocytochemistry, western blot, migration assay, invasion assay, and colony formation assay. The transduced cells showed a mesenchymal morphology. High levels of EMT-related proteins were also expressed. These results confirmed that the transduced cells underwent EMT. In addition, we observed an increased population of E-cadherin-downregulated HT29 cell line among the cells expressing colon CSC markers (CD133+ and CD44+) after EMT induction. E-cadherin-downregulated cells were morphologically like mesenchymal cells, and the number of CD133+- and CD44+-cells (CSC-like cells) increased. These cells can be used as stable models to study cancer cells and screening of antitumor therapeutics. 相似文献
99.
100.
Evaluation of 4‐bp insertion/deletion polymorphism within the 3′UTR of SGSM3 in bladder cancer using mismatch PCR‐RFLP method: A preliminary report
下载免费PDF全文
![点击此处可从《Journal of cellular biochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)