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91.
Animals are primarily limited by their capacity to acquire food, yet digestive performance also conditions energy acquisition, and ultimately fitness. Optimal foraging theory predicts that organisms feeding on patchy resources should maximize their food loads within each patch, and should digest these loads quickly to minimize travelling costs between food patches. We tested the prediction of high digestive performance in wandering albatrosses, which can ingest prey of up to 3 kg, and feed on highly dispersed food resources across the southern ocean. GPS-tracking of 40 wandering albatrosses from the Crozet archipelago during the incubation phase confirmed foraging movements of between 475-4705 km, which give birds access to a variety of prey, including fishery wastes. Moreover, using miniaturized, autonomous data recorders placed in the stomach of three birds, we performed the first-ever measurements of gastric pH and temperature in procellariformes. These revealed surprisingly low pH levels (average 1.50±0.13), markedly lower than in other seabirds, and comparable to those of vultures feeding on carrion. Such low stomach pH gives wandering albatrosses a strategic advantage since it allows them a rapid chemical breakdown of ingested food and therefore a rapid digestion. This is useful for feeding on patchy, natural prey, but also on fishery wastes, which might be an important additional food resource for wandering albatrosses. 相似文献
92.
Kato K Sasakawa H Kamiya Y Utsumi M Nakano M Takahashi N Yamaguchi Y 《Biochimica et biophysica acta》2008,1780(3):619-625
Although NMR spectroscopy has great potential to provide us with detailed structural information on oligosaccharides and glycoconjugates, the carbohydrate NMR analyses have been hampered by the severe spectral overlapping and the insufficiency of the conformational restraints. Recently, ultra-high field NMR spectrometers have become available for applications to structural analyses of biological macromolecules. Here we demonstrate that ultra-high fields offer not only increases in sensitivity and chemical shift dispersion but also potential benefits for providing unique information on chemical exchange and relaxation, by displaying NMR spectral data of oligosaccharide, glycoprotein, and glycolipid systems recorded at a 21.6 T magnetic field (corresponding to 920 MHz (1)H observation frequency). The ultra-high field NMR spectroscopy combined with sugar library and stable-isotope labeling approaches will open new horizons in structural glycobiology. 相似文献
93.
Noda Y Horikawa S Kanda E Yamashita M Meng H Eto K Li Y Kuwahara M Hirai K Pack C Kinjo M Okabe S Sasaki S 《The Journal of cell biology》2008,182(3):587-601
Trafficking of water channel aquaporin-2 (AQP2) to the apical membrane and its vasopressin and protein kinase A (PKA)-dependent regulation in renal collecting ducts is critical for body water homeostasis. We previously identified an AQP2 binding protein complex including actin and tropomyosin-5b (TM5b). We show that dynamic interactions between AQP2 and the actin cytoskeleton are critical for initiating AQP2 apical targeting. Specific binding of AQP2 to G-actin in reconstituted liposomes is negatively regulated by PKA phosphorylation. Dual color fluorescence cross-correlation spectroscopy reveals local AQP2 interaction with G-actin in live epithelial cells at single-molecule resolution. Cyclic adenosine monophosphate signaling and AQP2 phosphorylation release AQP2 from G-actin. In turn, AQP2 phosphorylation increases its affinity to TM5b, resulting in reduction of TM5b bound to F-actin, subsequently inducing F-actin destabilization. RNA interference-mediated knockdown and overexpression of TM5b confirm its inhibitory role in apical trafficking of AQP2. These findings indicate a novel mechanism of channel protein trafficking, in which the channel protein itself critically regulates local actin reorganization to initiate its movement. 相似文献
94.
A genic male sterile Chinese cabbage, Brassica campestris L. ssp. chinensis Makino, was examined using cytological and cytochemical methods to characterize the process of pollen abortion in this plant.
Thick sections of both fertile and sterile anthers at different developmental stages were stained using Toluidine Blue O,
Periodic Acid-Schiff’s (PAS) reaction and Sudan Black B to detect cytochemical changes that may occur in the distribution
of insoluble polysaccharide and lipid storage bodies. Pollen abortion in sterile anthers occurs at an early stage of microspore
development. During early microspore development, reductions in the number of starch grains in the connective tissue of fertile
anthers coincide with the accumulation of starch grains in cells of the anther wall. In the late microspore stage, a large
vacuole forms in the microspore, and tapetal cells synthesize and accumulate lipid droplets. The cellular organization of
tapetal cells in sterile anthers appears similar to that in fertile anthers, except for the absence of lipid droplets in cells
of sterile anthers and diffusely labeled tapetal polysaccharides, suggesting defects in nutrient storage.
Supported by National Natural Science Foundation of CHINA (30170060) 相似文献
95.
Nobata S Ogoshi M Takei Y 《American journal of physiology. Regulatory, integrative and comparative physiology》2008,294(5):R1544-R1553
Adrenomedullin (AM), known as a multifunctional hormone in mammals, forms a unique family of five paralogous peptides in teleost fish. To examine their cardiovascular effects using homologous AMs in eels, we isolated cDNAs encoding four eel AMs, and named AM1 (ortholog of mammalian AM), AM2, AM3 (paralog of AM2 generated only in teleost lineage), and AM5 according to the known teleost AM sequences. Unlike pufferfish, not only AM1 but AM2/3 and AM5 were expressed ubiquitously in various eel tissues. Synthetic mature AM1, AM2, and AM5 exhibited vasodepressor effects after intra-arterial injections, and the effects were more potent at dorsal aorta than at ventral aorta. This indicates that AMs preferentially act on peripheral resistance vessels rather than on branchial arterioles. The potency was in the order of AM2 = AM5 > AM1 in both freshwater (FW) and seawater (SW) eels, which is different from the result of mammals in which AM1 is as potent as, or more potent than, AM2 when injected peripherally. The minimum effective dose of AM2 and AM5 in eels was 1/10 that of AM1 in mammals. The hypotension reached 50% at 1.0 nmol/kg of AM2 and AM5, which is much greater than atrial natriuretic peptide (20%), another potent vasodepressor hormone. Even with such hypotension, AMs did not change heart rate in eels. In addition, AM1 increased blood pressure at ventral aorta and dorsal aorta immediately after an initial hypotension at 5.0 nmol/kg, but not with AM2 and AM5. These data strongly suggest that specific receptors for AM2 and AM5 exist in eels, which differ from the AM1 receptors identified in mammals. 相似文献
96.
Chato, a KRAB zinc-finger protein, regulates convergent extension in the mouse embryo 总被引:1,自引:0,他引:1
In Xenopus and zebrafish embryos, elongation of the anterior-posterior body axis depends on convergent extension, a process that involves polarized cell movements and is regulated by non-canonical Wnt signaling. The mechanisms that control axis elongation of the mouse embryo are much less well understood. Here, we characterize the ENU-induced mouse mutation chato, which causes arrest at midgestation and defects characteristic of convergent extension mutants, including a shortened body axis, mediolaterally extended somites and an open neural tube. The chato mutation disrupts Zfp568, a Krüppel-associated box (KRAB) domain zinc-finger protein. Morphometric analysis revealed that the definitive endoderm of mouse wild-type embryos undergoes cell rearrangements that lead to convergent extension during early somite stages, and that these cell rearrangements fail in chato embryos. Although non-canonical Wnt signaling is important for convergent extension in the mouse notochord and neural plate, the results indicate that chato regulates body axis elongation in all embryonic tissues through a process independent of non-canonical Wnt signaling. 相似文献
97.
Suzanne Dunel-Erb Claudine Chevalier Pierre Laurent Andr Bach Frdric Decrock Yvon Le Maho 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2001,129(4):933-947
To investigate the importance of body fuel depletion on gut rehabilitation after food deprivation, we compared the kinetics of jejunal mucosa alteration and restoration in rats that were refed after reaching different stages in body fuel depletion. Rats (P2) were refed while still in the so-called phase II, where body protein utilization is minimized, whereas rats (P3) were refed when they had reached the stage of increasing protein utilization (phase III). There was a significant decrease in total mass of intestine (P2, -30%; P3, -40%) and jejunal mucosa (P2, -52%; P3, -60%), as well in the size of the crypts (P2, -15%; P3, -36%) and villi (P2, -37%; P3, -55%). Structural changes of the mucosa included disappearance of some villi and a reduction in the size and number of crypts. Despite the larger morphological alterations in P3, the restoration of mucosa was as fast and complete after only 3 days of refeeding for both P2 and P3 rats. The respective roles of the mitosis pressure and of the lamina propria dynamics were studied. The rapid reversibility of the gut mucosal alterations due to fasting might constitute an integrative process. 相似文献
98.
C. Thouzeau J. -P. Robin Y. Le Maho Y. Handrich 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1999,169(8):612-620
The balance of protein versus lipid reserves utilization in fasting animals depends on their initial adiposity, a high prefasting
adiposity being associated with an efficient protein sparing during fasting. Yet it remains unclear if the level of energy
expenditure influences the efficiency of body protein sparing. We examined the effect of a high energy demand on body reserve
mobilization in barn owls (Tyto alba) fasting in the cold (5 °C). Changes in body composition of captive birds were determined during the three characteristic
phases of body fuel utilization of a long-term but reversible fast. Although showing a low prefasting adiposity (12%), barn
owls spared body protein in phase II as efficiently as the fattest species (contribution to energy expenditure of <9%). This
low protein utilization most probably results from an increased lipid mobilization in the cold. This argues for an influence
of a high energy demand on the relative efficiency of protein sparing. For lipids, the pattern of mobilization from tissue
sources is similar in barn owls to that of species fasting at thermoneutrality. For proteins, in contrast, and despite a low
decrease of the total body protein mass (16%), digestive tract and liver were affected most, with respective losses of 43%
and 62% at phase III. This could be another consequence of the interaction between high energy demand in the cold and fasting.
Indeed, high cold-induced thermoregulatory needs could result in selective preservation of organs involved in the thermoregulatory
process (muscles) to the detriment of lesser solicited organs such as those involved in digestion.
Accepted: 8 September 1999 相似文献
99.
M. Gauthier-Clerc B. Jaulhac Y. Frenot C. Bachelard H. Monteil Y. Le Maho Y. Handrich 《Polar Biology》1999,22(2):141-143
The seabird tick Ixodes uriae (White) is common in subantarctic areas and is likely to be a vector of pathogenic agents. This paper reports on the prevalence
of tick-infested adult king penguins (Aptenodytes patagonicus) and on antibodies to the Lyme disease agent (Borrelia burgdorferi sensu lato) in adult and chick penguins. Fifteen percent of the adults of the colony studied were tick infested. B. burgdorferi antibodies were detected in 14% of tick-infested adults' and in 6% of chicks' sera. The percentage of tick-infested adults
differed significantly according to location in the colony, the highest density occurring on the upper slopes of the colony.
The potential role of king penguins as B. burgdorferi reservoirs and the effect of this agent on king penguin populations remain unknown. The consequences for human health are
discussed.
Accepted: 15 March 1999 相似文献
100.
Caroline Gilbert Dominic McCafferty Yvon Le Maho Jean‐Marc Martrette Sylvain Giroud Stéphane Blanc André Ancel 《Biological reviews of the Cambridge Philosophical Society》2010,85(3):545-569
Huddling can be defined as “an active and close aggregation of animals”. It is a cooperative group behaviour, permitting individuals involved in social thermoregulation to minimize heat loss and thereby lower their energy expenditure, and possibly allowing them to reallocate the saved energy to other functions such as growth or reproduction. Huddling is especially important in the case of animals faced with high heat loss due to a high surface‐to‐volume ratio, poor insulation, or living in cold environments. Although numerous experimental studies have focused on the huddling behaviour of a wide range of species, to our knowledge, this is the first attempt to review the various implications of this widely used behavioural strategy. Huddling allows individuals to maximise energy savings by (1) decreasing their cold‐exposed body surface area, (2) reducing their heat loss through warming of ambient temperatures surrounding the group, and (3) eventually lowering their body temperature through physiological processes. Huddling provides substantial energy savings and is estimated to reduce energy expenditure by between 6 and 53%. Broad variations in the energetic benefits of huddling depend on the number of individuals and species involved in huddles, the ambient temperatures to which individuals are exposed and the density of the aggregations. It has been shown that huddling individuals have increased survival, a lower food intake, a decreased body mass loss, increased growth rate, reduced water loss, and/or a more constant body temperature together with a significant reduction in metabolic rate. Though huddling has been studied widely, this review reveals the intricacies of this adaptive behaviour. 相似文献