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41.
J. Hay W. Khan A.J.C. Mead D.V. Seal J.K. Sugden 《Letters in applied microbiology》1994,18(2):117-119
Commercially-available phenol red indicator, purified by adsorption chromatography was incorporated into lauryl sulphate broth (LSB) used in the membrane filtration method for the detection of Escherichia coli and other coliform bacteria. Relative to LSB containing the impure dye or its major contaminant, the purified phenol red provided clear visualization of discrete yellow colonies observed against a white background. The colonies remained stable for at least 24 h at 25°C under standard laboratory lighting conditions. This simple procedure will enhance the detection of coliforms in samples. 相似文献
42.
Christina Brahe Isabella Velonà Gerrit van der Steege Stefania Zappata Anneke Y. van de Veen Jan Osinga Carli M. J. Tops Riccardo Fodde P. Meera Khan Charles H. C. M. Buys Giovanni Neri 《Human genetics》1994,93(5):494-501
The locus responsible for the childhood-onset proximal spinal muscular atrophies (SMA) has recently been mapped to an area of 2–3 Mb in the region q12–13.3 of chromosome 5. We have used a series of radiation hybrids (RHs) containing distinct parts of the SMA region as defined by reference markers. A cosmid library was constructed from one RH. Thirteen clones were isolated and five of these were mapped within the SMA region. Both RH mapping and fluorescence in situ hybridization analysis showed that two clones map in the region between loci D5S125 and D5S351. One of the cosmids contains expressed sequences. Polymorphic dinucleotide repeats were identified in both clones and used for segregation analysis of key recombinant SMA families. One recombination between the SMA locus and the new marker 9Ic (D5S685) indicates that 9Ic is probably the closest distal marker. The absence of recombination between the SMA locus and marker Fc (D5S684) suggests that Fc is located close to the disease gene. These new loci should refine linkage analysis in SMA family studies and may facilitate the isolation of the disease gene. 相似文献
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44.
Measurements were made of fecal pellets for captive brow-antlered deer (Cervus eldi eldi) from different age and sex classes. Pellet measurements increased linearly with increases in age, except for length and width measurements in males. Differences in dimensions were statistically significant between sexes and among some age classes. This procedure may be useful in differentiating between the sexes, and to some extent between ages, of wild-living individuals. © 1993 Wiley-Liss, Inc. 相似文献
45.
Taposhi Hazra Sampa Kundu Subir Bera Tapan Chakraborty Mahasin Ali Khan 《植物分类学报:英文版》2023,61(6):1079-1090
The Ventilago Gaertn. (Rhamnaceae) is widely distributed in pantropical areas of Africa, Asia, and Australia. However, fossil records of this taxon are sparse, which limits understanding of the evolution and biogeographic history of the genus. In the present study, we report and describe two new fossil species of Ventilago, V. siwalika sp. nov. from the Miocene sediments of Himachal Pradesh, western Himalaya, and V. pliocenica sp. nov. from the Pliocene sediments of Jharkhand, eastern India based on single-winged samaras. Ventilago pliocenica is characterized by a prominent midvein, obtuse to sub-round apex with mucronate tip, longitudinal secondary veins extending the full length of the fruit, and reticulate nature of higher-order veins, the presence of equatorial rim, the hypanthium, and short pedicel. On the other hand, V. siwalika is characterized by a prominent midvein, obtuse to sub-round apex with mucronate tip, longitudinal secondary veins extending the full length of the fruit, and reticulate nature of higher-order veins. Our discovery represents the first unambiguous fossil record of single-winged samara of Ventilago from India and provides valuable insights into the evolution of this genus. In this paper, we also review its biogeographic history and add new information to understand its hypothetical migration route. Present and earlier records of Ventilago also suggest that this genus was a common forest element during Neogene (Miocene time) in Asia. 相似文献
46.
Khan MA 《Canadian journal of comparative medicine and veterinary science》1963,27(10):233-236
Although Apholate is used as a sexual sterilant of both sexes of houseflies (Musca domestica L.) it can not be used for ;systemic' chemosterilization of the prehypodermic larvae of the warble flies Hypoderma bovis (L.) and H. lineatum (De Vill.) because of its high toxicity to 4- to 5-month-old calves. An intramuscular dose of 2.5 mg./Kg. killed the calves in 5 to 7 days. The pathognomonic clinical signs were impaction of the rumen, anorexia, depression, and general weakness. The hematopoietic system was affected. There was marked leukocytopenia characterized by lymphocytopenia within 24 hours of Apholate injections. 相似文献
47.
Sequence requirements for the termination of rolling-circle replication of plasmid pT181 总被引:3,自引:0,他引:3
Most small multicopy plasmids of Gram-positive bacteria and many in Gram-negative bacteria replicate by a rolling-circle (RC) mechanism. The replication initiator proteins encoded by the RC plasmids and single-stranded bacteriophages of Escherichia coli have origin-specific nicking-closing activities that are required for the initiation and termination of RC replication. We have investigated the sequence requirements for termination of RC replication of plasmid pT181. The initiator nick site is located in the loop of a hairpin region (IRII) within the pT181 origin of replication. By mutational analysis, we have found that several nucleotides within the stem of IRII which are critical for the initiation activity are dispensable for termination of replication. We also demonstrate that nucleotides in the right arm of IRII, but not the left arm, are absolutely required for termination of RC replication. We have also identified specific nucleotides in IRII that are critical for its termination activity. The sequence of the right arm of the hairpin must be located downstream of the initiator nick site for termination, suggesting that termination requires a specific orientation of the initiator protein at the origin. 相似文献
48.
Seasonal studies were undertaken (August 1983–July 1984) toevaluate the algal distribution and ecology of the RiverDelimi, Jos Plateau, Nigeria at three stations: upstream, withlittle allochthonous input; midstream, receiving heavy domesticaffluents; downstream. A wide amplitude of variability wasdiscernible in physicochemical factors, and photosyntheticactivity and abundance of suspended algae in relation to thedegree of pollution along the first 7-km stretch of the river.Conductivity (µS cm-1) showed a 9-fold increase fromupstream (x = 35) to downstream. The standing crop(Chl a) fluctuated markedly, with 6-times higher valuesfor a polluted midstream site (x = 18.2 mg m-3). Production rate measurements (mg C m-3 d-1) showed significant variability with relatively lowvalues (x = 610) recorded for the upstream andhighest (x = 1050) at the midstream site. Apositive correlation was obtained between production rates andChl a for the polluted midstream zone. 相似文献
49.
APC mutation in the alternatively spliced region of exon 9 associated with late onset familial adenomatous polyposis 总被引:11,自引:0,他引:11
Rob B. van der Luijt Hans F. A. Vasen Carli M. J. Tops Cor Breukel Riccardo Fodde P. Meera Khan 《Human genetics》1995,96(6):705-710
Germ-line mutations in the adenomatous polyposis coli (APC) gene are responsible for familial adenomatous polyposis (FAP). Genotype-phenotype correlation studies in patients with FAP have demonstrated associations of certain variants of the disease with mutations at specific sites within the APC gene. In a large FAP family, we identified a frameshift mutation located in the alternatively spliced region of exon 9. Phenotypic studies of affected family members showed that the clinical course of FAP was delayed, with gastrointestinal symptoms and death from colorectal carcinoma occurring on average 25 and 20 years later than usual, respectively. The numbers of colorectal adenomas differed markedly among affected individuals and the location of colorectal cancer lay frequently in the proximal colon. Our findings suggest that the exon 9 mutation identified in the pedigree is associated with late onset of FAP. The atypical phenotype may be explained by the site of the mutation in the APC gene. Analysis of the APC protein product indicated that the exon 9 mutation did not result in a detectable truncated APC protein. Given the location of the mutation within an alternatively spliced exon of APC, it is conceivable that normal APC proteins are produced from the mutant allele by alternative splicing. 相似文献
50.
Allergic bronchopulmonary aspergillosis (ABPA): Studies on the general and specific humoral response
Serum specimens from 138 patients suffering from chronic respiratory disorders including 63 with allergic bronchopulmonary aspergillosis (ABPA), 20 with suspected ABPA, 25 with pulmonary tuberculosis, 14 with bronchial asthma, 10 with chronic bronchitis and 6 with miscellaneous pulmonary conditions were studied for circulating antibodies to Aspergillus. The ammonium sulfate test was employed with an iodine-125 labeled mycelial component derived from Aspergillus fumigatus. When compared to normal controls from the same area, this test indicated that sera from 82 per cent of patients with ABPA had elevated binding titers to the radiolabeled antigenic component. Immunodiffusion using a culture filtrate antigen from A. fumigatus, revealed precipitating antibody to this fungus in 89 per cent of sera from ABPA patients. The majority of patients with ABPA demonstrated marked elevations of total serum IgE, moderate elevations of serum IgA and IgD and slightly increased levels of IgG and IgM.This study was supported in part by Research Grant AI 10940 from the National Institutes of Health and by NHLI Contract N01-HL-3-2942(B), and forms a part of the Ph.D. thesis submitted by Z.U.K. to the University of Delhi. 相似文献