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21.
Karina Mondragon-Shem Waleed S. Al-Salem Louise Kelly-Hope Maha Abdeladhim Mohammed H. Al-Zahrani Jesus G. Valenzuela Alvaro Acosta-Serrano 《PLoS neglected tropical diseases》2015,9(2)
BackgroundThe sandfly Phlebotomus papatasi is the vector of Leishmania major, the main causative agent of Old World cutaneous leishmaniasis (CL) in Saudi Arabia. Sandflies inject saliva while feeding and the salivary protein PpSP32 was previously shown to be a biomarker for bite exposure. Here we used recombinant PpSP32 to evaluate human exposure to Ph. papatasi bites, and study the association between antibody response to saliva and CL in endemic areas in Saudi Arabia.ConclusionsOur findings suggest a possible correlation between the type of immunity generated by the exposure to sandfly bites and disease outcome. 相似文献
22.
Khadiga G. Adham Ahlam A. Alkhalifa Manal H. Farhood Nadia A. Aleisa Maha H. Daghestani 《Biologia》2014,69(6):817-824
Iron saccharate complex ISC is an iron supplement used to optimize erythropoiesis in cases of iron deficiencies. Because of the lack of major mechanisms of iron excretion, excess iron unbound to protective molecules is believed to be involved in catalyzing the generation of reactive oxygen species and induction of oxidative stress. This study employed ISC for the purpose of inducing iron overload and hence investigating the consequent iron toxicity, lipid peroxidation and antioxidant extent in a murine species. Male Wistar rats were given iron as intraperitoneal injections of ISC in subacute (0.2 mg Fe kg?1 for 2 weeks) and subchronic (0.1 mg Fe kg?1 for 4 weeks) doses. In iron-overloaded rats, enhanced hepatic iron accumulation (P > 0.001) attended by increased serum concentrations of malondialdehyde (MDA) (P > 0.001) and activities of antioxidant enzymes (superoxide dismutase SOD, catalase CAT and glutathione peroxidase GPx) (P > 0.001) was pointed out. The demonstrated antioxidant boost is attributed to a sense of equilibrium prompted by the potential of iron-induced oxidative stress to modify antioxidant defense capacity and to modulate susceptibility to oxidative stress. Rats seemed to constantly suffer from oxidative stress based on the consistent rise in MDA that was not overwhelmed by the elevated antioxidant input. The current findings are of informative value in drawing attention to the health hazards of applying higher doses of the commercially used iron supplement ISC. Data are virtually significant in elucidating the higher magnitude of subchronic than subacute iron overload in initiating oxidative stress and antioxidant defense. Both pathways proceeded in a time-dependent rather than dose-dependent manner. 相似文献
23.
Jennifer L. Jones Sarika Saraswati Ashley S. Block Cheryl F. Lichti Maha Mahadevan Alan B. Diekman 《Glycoconjugate journal》2010,27(2):227-236
Galectin-3 is a β-galactoside-binding protein involved in immunomodulation, cell interactions, cancer progression, and pathogenesis
of infectious organisms. We report the identification and characterization of galectin-3 in human semen. In the male reproductive
tract, the ~30 kDa galectin-3 protein was identified in testis, epididymis, vas deferens, prostate, seminal vesicle, and sperm
protein extracts. In seminal plasma, galectin-3 was identified in the soluble fraction and in prostasomes, cholesterol-rich,
membranous vesicles that are secreted by the prostate and incorporated into seminal plasma during ejaculation. Two-dimensional
immunoblot analysis of purified prostasomes identified five galectin-3 isoelectric variants with a pI range of 7.0 to 9.2.
Affinity purification and tandem mass spectrometry of β-galactoside-binding proteins from prostasomes confirmed the presence
of galectin-3 in prostasomes and identified a truncated galectin-3 variant. The intact galectin-3 molecule contains a carbohydrate
recognition domain and a non-lectin domain that interacts with protein and lipid moieties. The identification of a monovalent
galectin-3 fragment with conserved carbohydrate-binding activity indicates the functional relevance of this truncation and
suggests a regulatory mechanism for galectin-3 in prostasomes. Surface biotinylation studies suggested that galectin-3 and
the truncated galectin-3 variant are localized to the prostasome surface. Prostasomes are proposed to function in immunosuppression
and regulation of sperm function in the female reproductive tract, are implicated in facilitating sexually-transmitted infections,
and are indicated in prostate cancer progression. Given the overlap in functional significance, the identification of galectin-3
in prostasomes lays the groundwork for future studies of galectin-3 and prostasomes in reproduction, disease transmission,
and cancer progression. 相似文献
24.
25.
Spinosad is a bioinsecticide with a high degree of selective toxicity towards insects of different orders, but its toxicity
towards the two-spotted spider mite (TSSM), Tetranychus urticae Koch (Acari: Tetranychidae) is under debate. In this study, we compared the acaricidal properties of spinosad with the commercial
bioacaricide abamectin on the life stages of TSSM. Adulticide and ovicide bioassays were performed on a susceptible laboratory
strain using direct spraying of leaf disks with five rates of spinosad (20, 25, 30, 35 and 40 mg/l), five rates of abamectin
(0.125, 0.25, 0.5, 1 and 2.5 mg/l), sublethal concentrations or a combination of spinosad and abamectin. Both adulticidal
and ovicidal effects of spinosad against T. urticae in the laboratory were apparent, based on morality rates of the adults, reduction of female fecundity and death of offspring.
Abamectin was also found to significantly reduce female fecundity and killed offspring when applied directly on the eggs.
Interestingly, sublethal concentrations of spinosad reduced female fecundity stronger than abamectin. When a mixture of spinosad
and abamectin was applied at LC50, mortality was 74%, fecundity reduction was comparable to abamectin alone and egg hatching rate was lower than by either compound
alone. In conclusion, spinosad was more harmful than abamectin for TSSM life stages and the combined application is recommended. 相似文献
26.
Molecular and immunological evaluation of the expression of cancer/testis gene products in human colorectal cancer 总被引:1,自引:0,他引:1
Alves PM Lévy N Bouzourene H Viatte S Bricard G Ayyoub M Vuilleumier H Givel JC Halkic N Speiser DE Romero P Lévy F 《Cancer immunology, immunotherapy : CII》2007,56(6):839-847
Tumor-specific gene products, such as cancer/testis (CT) antigens, constitute promising targets for the development of T cell
vaccines. Whereas CT antigens are frequently expressed in melanoma, their expression in colorectal cancers (CRC) remains poorly
characterized. Here, we have studied the expression of the CT antigens MAGE-A3, MAGE-A4, MAGE-A10, NY-ESO-1 and SSX2 in CRC
because of the presence of well-described HLA-A2-restricted epitopes in their sequences. Our analyses of 41 primary CRC and
14 metastatic liver lesions confirmed the low frequency of expression of these CT antigens. No increased expression frequencies
were observed in metastatic tumors compared to primary tumors. Histological analyses of CRC samples revealed heterogeneous
expression of individual CT antigens. Finally, evidence of a naturally acquired CT antigen-specific CD8+ T cell response could be demonstrated. These results show that the expression of CT antigens in a subset of CRC patients
induces readily detectable T cell responses. 相似文献
27.
Godefroy E Wang Y Souleimanian NE Scotto L Stevanovic S Chen YT Valmori D Ayyoub M 《Cancer immunology, immunotherapy : CII》2007,56(8):1183-1192
Proteins encoded by genes of the SSX family are specifically expressed in tumors and are therefore relevant targets for cancer immunotherapy. One of the first identified family members, SSX-1, is expressed in a large fraction of synovial sarcomas as a fusion protein together with the product of the SYT gene. In addition, the full-length SSX-1 antigen is frequently expressed in tumors of several other histological types such as sarcoma, melanoma, hepatocellular carcinoma, ovarian cancer and myeloma. To date, however, SSX-1 specific T cell responses have not been investigated and no SSX-1 derived T cell epitopes have been described. Here, we have assessed the presence of CD4(+) T cells directed against the SSX-1 antigen in circulating lymphocytes of cancer-free individuals. After a single in vitro stimulation with a pool of peptides spanning the entire SSX-1 protein we could detect and isolate SSX-1-specific CD4(+) T cells from 5/5 donors analyzed. SSX-1-specific polyclonal populations isolated from these cultures recognized peptides located in three distinct regions of the protein containing clusters of sequences with significant predicted binding to frequently expressed MHC class II alleles. Characterization of specific clonal CD4(+) T cell populations derived from one donor allowed the identification of several naturally processed epitopes recognized in association with HLA-DR. These data document the existence of a significant repertoire of CD4(+) T cells specific for SSX-1 derived sequences in circulating lymphocytes of any individual that can be exploited for the development of both passive and active immunotherapeutic approaches to control disease evolution in cancer patients. 相似文献
28.
Kammoun-Krichen M Bougacha-Elleuch N Makni K Rebai M Peraldi-Roux S Rebai A Mnif M Abid M Jouida J Ayadi H 《European cytokine network》2007,18(4):196-200
Autoimmune thyroid diseases (AITDs), including Graves' disease (GD) and autoimmune hypothyroidism (AH), are inherited as complex traits. Among the genes contributing to AITDs susceptibility are genes of the IL-1 family. IL-1 regulates T and B lymphocyte maturation, including the induction of several cytokines and cytokine receptors. Therefore, disturbances of this balance may not only play a role in inflammation but also in the pathogenesis of autoimmunity. In order to investigate genetic association of IL-1 gene polymorphisms with AITDs, we performed both a familial study in a large Tunisian pedigree with high prevalence of AITDs (64 patients and 176 controls), and a case-control study (131 GD unrelated patients and 225 healthy controls). PCR and PCR-RFLP methods were used to analyse respectively a VNTR in the IL-1RN gene and three SNPs in both IL-1B genes (-511 C/T and +3954 C/T) and IL-1A (-889 C/T). The family-based association study showed an association of the IL-1B+3954 C/T polymorphism (p=0.02) and two haplotypes IL-1RN*3/C/T/T and IL-1RN*1/C/T/T (p=0.009 and p=0.047 respectively) with AITDs. The case-control study is the first study revealing a significant association of the IL-1A-889 C/T polymorphism (chi2=10.23; p=0.0014) with susceptibility to GD. Our data suggest that the IL-1 gene cluster may harbour susceptibility genes for AITDs and GD pathogenesis in the Tunisian population. 相似文献
29.
Al-Saleh Iman Alrushud Nujud Alnuwaysir Hissah Elkhatib Rola Shoukri Mohamed Aldayel Fouad Bakheet Razan Almozaini Maha 《Biometals》2022,35(1):125-145
BioMetals - The role of micronutrient deficiency in the pathogenesis of COVID-19 has been reviewed in the literature; however, the data are limited and conflicting. This study investigated the... 相似文献
30.
Osama Mohamed ElShfei El-Azazy Nadra-Elwgoud Mohamed Ibrahim Abdou Amal Iskander Khalil Maha Khaled Al-Batel Qais Abdulrazak Habeeb Majeed Adawia Abdul-Ruhman Henedi Laila Mohamed Azad Tahrani 《The Korean journal of parasitology》2015,53(3):279-287
Stray cats are a common feature roaming the streets and alleys of Kuwait; they could be a source of parasites, including trematodes, that affect humans. A survey was conducted to identify feline trematodes and throw the light on their public health significance in Kuwait. Out of 240 stray cats trapped from different localities of Kuwait from June 2011 to May 2012, 59 (24.6%) were found to be infected with 14 species of trematodes. The most common were trematodes of the genus Heterophyes, particularly H. heterophyes and H. dispar that were found in respectively 15.8% and 10.8% of the cats examined. Other trematodes recorded, with lower prevalences, were Heterophyes nocens (2.9%), Haplorchis taichui (3.8%), Stictodora sawakinensis (2.1%), Stellantchasmus falcatus (1.6%), Echinochasmus japonicus (1.6%), and Mesostephanus dottrensi (1.3%). Centrocestus cuspidatus, Galactosomum fregatae, Ascocotyle sp., Mesostephanus appendiculatus, Haplorchis yokogawai, and Pygidiopsis genata showed the lowest prevalence (0.4%) and intensity. The majority of the trematodes are recorded for the first time in Kuwait and even in the Gulf region. The study reveals that stray cats are good indicators of fish-borne trematodes in the environment. As all trematodes recovered are zoonotic, their significance to public health should be considred. 相似文献