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Charalambos Magoulas Ada Loverre-Chyurlia Sumaia Abukashawa Laure Bally-Cuff Donal A. Hickey 《Journal of molecular evolution》1993,36(3):234-242
Summary Previous studies have demonstrated that the expression of the -amylase gene is repressed by dietary glucose in Drosophila melanogaster. Here, we show that the -amylase gene of a distantly related species, D. virilis, is also subject to glucose repression. Moreover, the cloned amylase gene of D. virilis is shown to be glucose repressible when it is transiently expressed in D. melanogaster larvae. This cross-species, functional conservation is mediated by a 330-bp promoter region of the D. virilis amylase gene. These results indicate that the promoter elements required for glucose repression are conserved between distantly related Drosophila species. A sequence comparison between the amylase genes of D. virilis and D. melanogaster shows that the promoter sequences diverge to a much greater degree than the coding sequences. The amylase promoters of the two species do, however, share small clusters of sequence similarity, suggesting that these conserved cis-acting elements are sufficient to control the glucose-regulated expression of the amylase gene in the genus Drosophila.Offprint requests to: D.A. Hickey 相似文献
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Analysis of mitochondrial DNA restriction fragment length polymorphism in
European anchovy (Engraulis encrasicolus) revealed a large number of
mitotypes that form two distinct clusters (phylads). Phylad A consists of
one common mitotype and many rare secondary mitotypes that are one
mutational step removed from the main type. Nucleotide diversity and number
of homoplasious changes are low. Phylad B has a complex pattern of mitotype
connectedness, high nucleotide diversity, and a large number of
homoplasious changes. It is suggested that the two phylads evolved in
isolation from each other and that present coexistence is the result of a
secondary contact. Moreover, phylad A has a "star" phylogeny, which
suggests that it has evolved in a population that experienced a drastic
bottleneck followed by an explosion of size. Phylad A is practically the
only phylad present in the Black Sea, with its frequency dropping to 85% in
the northern Aegean, and to 40% in the rest of Mediterranean and the Bay of
Biscay. The Black Sea is, therefore, the most likely place of origin of
phylad A. Molecular data are consistent with a population bottleneck in the
Black Sea during the last glaciation event and a subsequent exit of phylad
A with the outflow into the Aegean following the ice melting. Phylogenetic
analysis of anchovy mtDNA provides a reconstruction of population history
in the Mediterranean, which is consistent with the geological information.
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Christakis Christos A. Polymenakou Paraskevi N. Mandalakis Manolis Nomikou Paraskevi Kristoffersen Jon Bent Lampridou Danai Kotoulas Georgios Magoulas Antonios 《Extremophiles : life under extreme conditions》2018,22(5):825-825
Extremophiles - In the original publication there is a mistake in the supplementary material. The correct supplementary material is provided in this correction article. 相似文献
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Bo Yuan Tamar Harel Shen Gu Pengfei Liu Lydie Burglen Sandra Chantot-Bastaraud Violet Gelowani Christine?R. Beck Claudia?M.B. Carvalho Sau?Wai Cheung Andrew Coe Valérie Malan Arnold Munnich Pilar?L. Magoulas Lorraine Potocki James?R. Lupski 《American journal of human genetics》2015,96(5):691-692
The genomic duplication associated with Potocki-Lupski syndrome (PTLS) maps in close proximity to the duplication associated with Charcot-Marie-Tooth disease type 1A (CMT1A). PTLS is characterized by hypotonia, failure to thrive, reduced body weight, intellectual disability, and autistic features. CMT1A is a common autosomal dominant distal symmetric peripheral polyneuropathy. The key dosage-sensitive genes RAI1 and PMP22 are respectively associated with PTLS and CMT1A. Recurrent duplications accounting for the majority of subjects with these conditions are mediated by nonallelic homologous recombination between distinct low-copy repeat (LCR) substrates. The LCRs flanking a contiguous genomic interval encompassing both RAI1 and PMP22 do not share extensive homology; thus, duplications encompassing both loci are rare and potentially generated by a different mutational mechanism. We characterized genomic rearrangements that simultaneously duplicate PMP22 and RAI1, including nine potential complex genomic rearrangements, in 23 subjects by high-resolution array comparative genomic hybridization and breakpoint junction sequencing. Insertions and microhomologies were found at the breakpoint junctions, suggesting potential replicative mechanisms for rearrangement formation. At the breakpoint junctions of these nonrecurrent rearrangements, enrichment of repetitive DNA sequences was observed, indicating that they might predispose to genomic instability and rearrangement. Clinical evaluation revealed blended PTLS and CMT1A phenotypes with a potential earlier onset of neuropathy. Moreover, additional clinical findings might be observed due to the extra duplicated material included in the rearrangements. Our genomic analysis suggests replicative mechanisms as a predominant mechanism underlying PMP22-RAI1 contiguous gene duplications and provides further evidence supporting the role of complex genomic architecture in genomic instability. 相似文献
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Investigation on Toxicity and Teratogenicity in Rats of a Retinoid‐Polyamine Conjugate with Potent Anti‐Inflammatory Properties
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Theodoros Petridis Dimitra Giannakopoulou Vassiliki Stamatopoulou Katerina Grafanaki Christos G. Kostopoulos Helen Papadaki Nikos K. Karamanos Stathianna Douroumi Dionysios Papachristou George E. Magoulas Dionissios Papaioannou Denis Drainas 《Birth defects research. Part B, Developmental and reproductive toxicology》2016,107(1):32-44
Previous studies have shown that N1,N12‐bis(all‐trans‐retinoyl)spermine (RASP), a retinoid analog, inhibits RNase P activity and angiogenesis in the chicken embryo chorioallantoic membrane, demonstrates anti‐tumor activity on prostate cancer cells, and acts as anti‐inflammatory agent, being more effective and less toxic than all‐trans retinoic acid. In an attempt to further characterize the biological profile of RASP, we tested its effects on organ toxicity and teratogenicity by daily oral gavage of RASP at a level of 50 mg/Kg of body weight in two generations of rats. We found that this compound does not induce changes to the body growth, the appearance of physical features, and the animal's reflexes. Additionally, no substantial histopathological lesions were found in brain, heart, lung, thymus, liver, thyroid gland, adrenal gland, pituitary gland, kidneys, spleen, skin, femora, prostate, testis, epididymis, vagina, uterus, and ovaries of RASP‐treated animals. These results suggest RASP, as a promising lead compound for the treatment of several dermatological disorders and certain cancer types, has apparently minimal toxic side‐effects as revealed in this two‐generation reproduction study in rats. 相似文献
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