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991.
Although not yet fully understood, reduced sensitivity of tree growth to temperature at high northern latitudes during the last ? 40 years is often linked to concurrent anthropogenic changes of atmospheric composition and global warming. The idea that a temporal localization of the problem could improve its understanding initiated a search for erratic growth‐patterns in earlier periods of high quality dendrochronological archives. An extensive network of maximum latewood density (MXD) measurements from northern Fennoscandia likely represents one of the most reliable regional summer‐temperature reconstructions. The strong coherence between proxy and instrumental data is, however, interrupted by a short, but significant correlation decrease from ? 1900 till 1925, a period of distinct summer‐temperature warming. Here we analyze this early 20th century divergence period (EDP). We therefore use long instrumental station records and tree‐ring density chronologies including 878 Pinus sylvestris and 126 Picea abies samples. Our results indicate that EDP was accompanied by a simultaneous decline of inter‐site and inter‐station correlations. This could be ascribed to substantially reduced inter‐annual summer temperature variability from 1905–1919. Stable correlations of the MXD network with high‐pass filtered sea level pressure and precipitation records imply tree‐growth to be additionally controlled by other factors, e.g. light conditions, in periods of low summer temperature variability. Within the scope of this study, the causes for EDP could be confined to a limited area and a short period. Calibration of proxy data and reconstruction skills thus remain unaffected in this case of divergence. 相似文献
992.
Pierluca Coiro Luminita Stoenica Ulf Strauss Anja Ursula Br?uer 《The Journal of biological chemistry》2014,289(36):24956-24970
The transmembrane protein plasticity-related genes 3 and 5 (PRG3 and PRG5) increase filopodial formation in various cell lines, independently of Cdc42. However, information on the effects of PRG5 during neuronal development is sparse. Here, we present several lines of evidence for the involvement of PRG5 in the genesis and stabilization of dendritic spines. First, PRG5 was strongly expressed during mouse brain development from embryonic day 14 (E14), peaked around the time of birth, and remained stable at least until early adult stages (i.e. P30). Second, on a subcellular level, PRG5 expression shifted from an equal distribution along all neurites toward accumulation only along dendrites during hippocampal development in vitro. Third, overexpression of PRG5 in immature hippocampal neurons induced formation of spine-like structures ahead of time. Proper amino acid sequences in the extracellular domains (D1 to D3) of PRG5 were a prerequisite for trafficking and induction of spine-like structures, as shown by mutation analysis. Fourth, at stages when spines are present, knockdown of PRG5 reduced the number but not the length of protrusions. This was accompanied by a decrease in the number of excitatory synapses and, consequently, by a reduction of miniature excitatory postsynaptic current frequencies, although miniature excitatory postsynaptic current amplitudes remained similar. In turn, overexpressing PRG5 in mature neurons not only increased Homer-positive spine numbers but also augmented spine head diameters. Mechanistically, PRG5 interacts with phosphorylated phosphatidylinositols, phospholipids involved in dendritic spine formation by different lipid-protein assays. Taken together, our data propose that PRG5 promotes spine formation. 相似文献
993.
Xiaoling Dun Wenhao Shen Kaining Hu Zhengfu Zhou Shengqian Xia Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma Jinxing Tu Tingdong Fu Ulf Lagercrantz 《Plant physiology》2014,166(3):1403-1419
Gene duplication followed by functional divergence in the event of polyploidization is a major contributor to evolutionary novelties. The Brassica genus evolved from a common ancestor after whole-genome triplication. Here, we studied the evolutionary and functional features of Brassica spp. homologs to Tic40 (for translocon at the inner membrane of chloroplasts with 40 kDa). Four Tic40 loci were identified in allotetraploid Brassica napus and two loci in each of three basic diploid Brassica spp. Although these Tic40 homologs share high sequence identities and similar expression patterns, they exhibit altered functional features. Complementation assays conducted on Arabidopsis thaliana tic40 and the B. napus male-sterile line 7365A suggested that all Brassica spp. Tic40 homologs retain an ancestral function similar to that of AtTic40, whereas BolC9.Tic40 in Brassica oleracea and its ortholog in B. napus, BnaC9.Tic40, in addition, evolved a novel function that can rescue the fertility of 7365A. A homologous chromosomal rearrangement placed bnac9.tic40 originating from the A genome (BraA10.Tic40) as an allele of BnaC9.Tic40 in the C genome, resulting in phenotypic variation for male sterility in the B. napus near-isogenic two-type line 7365AB. Assessment of the complementation activity of chimeric B. napus Tic40 domain-swapping constructs in 7365A suggested that amino acid replacements in the carboxyl terminus of BnaC9.Tic40 cause this functional divergence. The distribution of these amino acid replacements in 59 diverse Brassica spp. accessions demonstrated that the neofunctionalization of Tic40 is restricted to B. oleracea and its derivatives and thus occurred after the divergence of the Brassica spp. A, B, and C genomes.Polyploidy or whole-genome duplication is thought to be a prominent evolutionary force in eukaryotes (Wolfe, 2001; Udall and Wendel, 2006), especially for flowering plants (Blanc and Wolfe, 2004; Van de Peer et al., 2009). Almost 95% of angiosperms show evidence of having undergone at least one round of whole-genome duplication in their evolutionary history, suggesting that most extant diploid flowering plants have evolved from ancient polyploids (Cui et al., 2006; Soltis et al., 2009). Gene duplications in the event of polyploidization provide sources for evolutionary novelties that could benefit plants (Lukens et al., 2004; Chen, 2007). Divergence after gene duplication could result in three primary evolutionary fates of duplicated genes: pseudogenization, neofunctionalization, and subfunctionalization (Force et al., 1999; Conant and Wolfe, 2008; Liu and Adams, 2010). Pseudogenization implies that duplicated genes with redundant functions lose their function by accumulating negative mutations; neofunctionalization denotes that the redundant gene evolves a new adaptive function; while subfunctionalization causes the duplicated genes to adopt a different part of the function of an ancestral gene (Rodríguez-Trelles et al., 2003; Flagel and Wendel, 2009; Liu and Adams, 2010).The Brassica genus consists of three basic diploid species: Brassica rapa (AA; n = 10), Brassica nigra (BB; n = 8), and Brassica oleracea (CC; n = 9), and their derivative allotetraploid species: Brassica juncea (AABB; n = 18), Brassica napus (AACC; n = 19), and Brassica carinata (BBCC; n = 17; Beilstein et al., 2006). Comparative genetic mapping demonstrated that these Brassica spp., which diverged from Arabidopsis thaliana approximately 20 to 40 million years ago (Lagercrantz and Lydiate, 1996; Blanc et al., 2003; Town et al., 2006), descended from a common ancestor after whole-genome triplication (Parkin et al., 2002). Collinear comparison showed that for each of 24 ancestral genomic blocks defined in the ancestral karyotype in A. thaliana, three syntenic copies were identified in each of the diploid Brassica spp. genomes, with only one exception (Schranz et al., 2006; Wang et al., 2011; Cheng et al., 2013). Fractionation (gene loss from homologous genomic regions) and chromosomal rearrangements were prevalent in the diploidization process of the hexaploid Brassica spp. common ancestor (Lagercrantz, 1998; Town et al., 2006; Ziolkowski et al., 2006; Mun et al., 2009). Based on gene density differences caused by varying gene loss rates in the three collinear genomic block copies, these genomic blocks were classified into three subgenomes in the diploid Brassica spp. genomes: the least fractionated (LF), the medium fractionated (MF1), and the most fractionated (MF2) subgenomes (Wang et al., 2011; Tang and Lyons, 2012; Cheng et al., 2013). The different rates of gene loss of the three subgenomes support a two-step origin of the Brassiceae ancestral genome involving a tetraploidization process followed by substantial fractionation of the subgenomes MF1 and MF2 and more recently hybridization with the third subgenome LF to form a hexaploid (Wang et al., 2011; Tang et al., 2012). Although collinearity and changes in genomic structure, including duplications, deletions, and rearrangements of the Brassica spp. and A. thaliana are well studied, there is limited knowledge of the molecular and functional divergence of duplicated or homologous genes in the Brassica spp.To utilize heterosis in B. napus breeding, hybrid production is based mainly on male sterility. Currently, the recessive epistatic genic male-sterile three-type line system 7365ABC is widely used for oilseed heterosis due to its advantages, producing 100% sterile offspring for realizing the triple-cross hybrid (Huang et al., 2007; Xia et al., 2012). The male sterility in this system is controlled by two genes, a recessive male sterile gene Bnms3 and a epistatic gene BnRf (Zhou et al., 2012). Bnms3 was recently reported to be a homolog to A. thaliana Tic40 (Dun et al., 2011). Tic40 was identified as a member of the TIC (for translocon at the inner envelope membrane of chloroplasts) complex that functions as a cochaperone to coordinate Tic110 and the stromal chaperone heat shock protein93 (Hsp93) (Chou et al., 2003, 2006). The A. thaliana tic40 mutant displayed a chlorotic phenotype throughout development (Chou et al., 2003) but a male-fertile phenotype with mature pollen grains (Dun et al., 2011). Interestingly, one allele of Bnms3, BnaC.Tic40, can rescue the fertility of the B. napus male-sterile line 7365A (Dun et al., 2011).In this study, we identified and characterized Tic40 homologs in B. napus and three basic diploid Brassica spp. We suggested that neofunctionalization of BnaC9.Tic40 after the divergence of the Brassica spp. A, B, and C genomes was caused by amino acid replacements in the C terminus of BnaC9.Tic40. In addition, we validated that the allelic genes BnaC9.Tic40 (equivalent to BnaC.Tic40 as described by Dun et al. [2011]) and bnac9.tic40 originate from the C and A genomes, respectively, and became allelic due to a homologous chromosomal rearrangement in B. napus. These results provide further knowledge for the effective utilization of the restoring gene of 7365A and a better insight into the functional divergence of homologous duplicated genes in paleoploid Brassica spp. 相似文献
994.
Ben Lu Kevin Kwan Yaakov A Levine Peder S Olofsson Huan Yang Jianhua Li Sonia Joshi Haichao Wang Ulf Andersson Sangeeta S Chavan Kevin J Tracey 《Molecular medicine (Cambridge, Mass.)》2014,20(1):350-358
The mammalian immune system and the nervous system coevolved under the influence of cellular and environmental stress. Cellular stress is associated with changes in immunity and activation of the NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome, a key component of innate immunity. Here we show that α7 nicotinic acetylcholine receptor (α7 nAchR)-signaling inhibits inflammasome activation and prevents release of mitochondrial DNA, an NLRP3 ligand. Cholinergic receptor agonists or vagus nerve stimulation significantly inhibits inflammasome activation, whereas genetic deletion of α7 nAchR significantly enhances inflammasome activation. Acetylcholine accumulates in macrophage cytoplasm after adenosine triphosphate (ATP) stimulation in an α7 nAchR-independent manner. Acetylcholine significantly attenuated calcium or hydrogen oxide–induced mitochondrial damage and mitochondrial DNA release. Together, these findings reveal a novel neurotransmitter-mediated signaling pathway: acetylcholine translocates into the cytoplasm of immune cells during inflammation and inhibits NLRP3 inflammasome activation by preventing mitochondrial DNA release. 相似文献
995.
Green algae are major components of biological soil crusts in alpine habitats. Together with cyanobacteria, fungi and lichens, green algae form a pioneer community important for the organisms that will succeed them. In their high altitudinal habitat these algae are exposed to harsh and strongly fluctuating environmental conditions, mainly intense irradiation, including ultraviolet radiation, and lack of water leading to desiccation. Therefore, green algae surviving in these environments must have evolved with either avoidance or protective strategies, as well as repair mechanisms for damage. In this review we have highlighted these mechanisms, which include photoprotection, photochemical quenching, and high osmotic values to avoid water loss, and in some groups flexibility of secondary cell walls to maintain turgor pressure even in water-limited situations. These highly specialized green algae will serve as good model organisms to study desiccation tolerance or photoprotective mechanisms, due to their natural capacity to withstand unfavorable conditions. We point out the urgent need for modern phylogenetic approaches in characterizing these organisms, and molecular methods for analyzing the metabolic changes involved in their adaptive strategies. 相似文献
996.
Ulf Toelch Matthew J. Bruce Lesley Newson Peter J. Richerson Simon M. Reader 《Proceedings. Biological sciences / The Royal Society》2014,281(1776)
Copying others appears to be a cost-effective way of obtaining adaptive information, particularly when flexibly employed. However, adult humans differ considerably in their propensity to use information from others, even when this ‘social information’ is beneficial, raising the possibility that stable individual differences constrain flexibility in social information use. We used two dissimilar decision-making computer games to investigate whether individuals flexibly adjusted their use of social information to current conditions or whether they valued social information similarly in both games. Participants also completed established personality questionnaires. We found that participants demonstrated considerable flexibility, adjusting social information use to current conditions. In particular, individuals employed a ‘copy-when-uncertain’ social learning strategy, supporting a core, but untested, assumption of influential theoretical models of cultural transmission. Moreover, participants adjusted the amount invested in their decision based on the perceived reliability of personally gathered information combined with the available social information. However, despite this strategic flexibility, participants also exhibited consistent individual differences in their propensities to use and value social information. Moreover, individuals who favoured social information self-reported as more collectivist than others. We discuss the implications of our results for social information use and cultural transmission. 相似文献
997.
Anette Peterson Lena Hanberger Karin ?kesson Mats Bojestig Boel Andersson G?re Ulf Samuelsson 《PloS one》2014,9(5)
Background
Several studies show that good metabolic control is important for children and adolescents with type 1 diabetes. In Sweden, there are large differences in mean haemoglobin A1c (HbA1c) in different hospitals and difficulties implementing national guidelines in everyday practice. This study shows how the participation in an improvement collaborative could facilitate improvements in the quality of care by paediatric diabetes teams. The Swedish paediatric diabetes quality registry, SWEDIABKIDS was used as a tool and resource for feedback and outcome measures.Methods
Twelve teams at paediatric diabetes centres, caring for 30% (2302/7660) of patients in Sweden, participated in an 18-month quality improvement program. Each team defined treatment targets, areas needing improvement, and action plans. The main outcome was the centre patients'' mean HbA1c levels, but other clinical variables and change concepts were also studied. Data from the previous six months were compared with the first six months after starting the program, and the long-term follow up after another eleven months.Results
All centres reduced mean HbA1c during the second and third periods compared with the first. The mean reduction for all was 3·7 mmol/mol (p<0.001), compared with non-participating centres who improved their mean HbA1c with 1·7 mmol/mol during the same period. Many of the participating centres reduced the frequency of severe hypoglycaemia and/or ketoacidosis, and five centres reached their goal of ensuring that all patients had some sort of physical activity at least once weekly. Change concepts were, for example, improved guidelines, appointment planning, informing the patients, improving teamwork and active use of the registry, and health promotion activities.Conclusions
By involving paediatric diabetes teams in a quality improvement collaborative together with access to a quality register, the quality of paediatric diabetes care can improve, thereby contributing to a reduced risk of late complications for children and adolescents with diabetes. 相似文献998.
Babs E. Verstrepen Herman Oostermeijer Zahra Fagrouch Melanie van Heteren Henk Niphuis Tom Haaksma Ivanela Kondova Willy M. Bogers Marina de Filette Niek Sanders Linda Stertman Sofia Magnusson Orsolya L?rincz Julianna Lisziewicz Luisa Barzon Giorgio Palù Michael S. Diamond Stefan Chabierski Sebastian Ulbert Ernst J. Verschoor 《PloS one》2014,9(11)
The mosquito-borne West Nile virus (WNV) causes human and animal disease with outbreaks in several parts of the world including North America, the Mediterranean countries, Central and East Europe, the Middle East, and Africa. Particularly in elderly people and individuals with an impaired immune system, infection with WNV can progress into a serious neuroinvasive disease. Currently, no treatment or vaccine is available to protect humans against infection or disease. The goal of this study was to develop a WNV-vaccine that is safe to use in these high-risk human target populations. We performed a vaccine efficacy study in non-human primates using the contemporary, pathogenic European WNV genotype 1a challenge strain, WNV-Ita09. Two vaccine strategies were evaluated in rhesus macaques (Macaca mulatta) using recombinant soluble WNV envelope (E) ectodomain adjuvanted with Matrix-M, either with or without DNA priming. The DNA priming immunization was performed with WNV-DermaVir nanoparticles. Both vaccination strategies successfully induced humoral and cellular immune responses that completely protected the macaques against the development of viremia. In addition, the vaccine was well tolerated by all animals. Overall, The WNV E protein adjuvanted with Matrix-M is a promising vaccine candidate for a non-infectious WNV vaccine for use in humans, including at-risk populations. 相似文献
999.
Mats Halldin Kerstin Brismar Per Fahlstadius Max Vikstr?m Ulf de Faire Mai-Lis Hellénius 《PloS one》2014,9(12)
Background and Aims
The metabolic syndrome (MetS) is associated with an increased risk for left ventricular hypertrophy (LVH) and cardiovascular mortality. The aim of this study was to investigate potential influences from insulin-like growth factor-1 (IGF-1) and IGF binding protein-1 (IGFBP-1) on the relationship between the MetS and LVH, also taking into account the role of physical activity (PA), use of oestrogen and gender.Methods and Results
In a population-based cross-sectional study of 60-year-old men (n = 1822) and women (n = 2049) participants underwent physical examination and laboratory tests, including electrocardiography (ECG), and completed an extensive questionnaire. Women showed higher levels of IGFBP-1 than men (37.0 vs. 28.0 µg/l, p<0.001), and women with LVH had lower levels of IGFBP-1 than women without LVH (31.0 µg/l vs. 37.0 µg/l, p<0.001). Furthermore, women with low levels of IGFBP-1 had a significantly increased risk of having LVH (crude OR≈2.5). When stratifying for PA and oestrogen, respectively, a weaker association between IGFBP-1 and LVH was demonstrated in physically active men and women, compared to inactive individuals, as well as in women using oestrogen, compared to non-users.Conclusion
In a representative sample of 60-year-old Swedish men and women, the main findings were higher levels of IGFBP-1 in women than in men; lower levels of IGFBP-1 in women with LVH, compared to women without LVH; and an increased risk of having LVH in women with low levels of IGFBP-1. The association between IGFBP-1 and LVH was diminished in physically active men and women, as well as in women using oestrogen. 相似文献1000.
Mohsen Besharat Pour Anna Bergstr?m Matteo Bottai Jessica Magnusson Inger Kull Magnus Wickman Tahereh Moradi 《PloS one》2014,9(10)