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951.
Our understanding of the accuracy of tRNA selection on the messenger RNA programmed ribosome has recently increased dramatically because of high-resolution crystal structures of the ribosome, cryo-electron microscopy reconstructions of its functional complexes, and fast kinetics experiments. Application of single-molecule spectroscopy with fluorescence resonance energy transfer to studies of tRNA selection by the ribosome has also provided new, albeit controversial, insights. Interestingly, when the fundamental trade-off between rate and accuracy in substrate-selective biosynthetic reactions is taken into account, some aspects of the current models of ribosome function appear strikingly suboptimal in the context of growing bacterial cells. 相似文献
952.
Laura E. Stummer Jennifer A. Weller Magnus L. Johnson Isabelle M. Côté 《Animal behaviour》2004,68(1):145-150
Little is known of how individuals find each other in interspecific mutualisms involving free-living partners. We tested the importance of two factors, namely body size and the presence of a lateral body stripe, in the recognition of cleanerfish by their fish clients. Clients on an Indonesian reef flat readily approached wooden models of the bluestreak cleaner wrasse, Labroides dimidiatus, which varied in size and stripe characteristics. The composition of the clientele of models was not significantly different from that of natural cleaning stations, suggesting that fish visiting the models were seeking to be cleaned. Normal-sized models of cleaner wrasses attracted significantly more clients, which showed more intense interest and stayed with the models for significantly longer, than super-sized models. For normal-sized models, the number of clients increased as the length of the cleaner's lateral stripe increased (from 0, to 44, 67 and 100% of body length). However, there was no effect of stripe length on client numbers for super-sized models. Client interest also did not vary with stripe length for models of either size. Small body size and the presence of a lateral stripe therefore appear to be long-distance signals that their bearer may be a cleaner, but after initial attraction, client interest is maintained by other cues. Alternative short-distance signals may include colour, other visual signals such as cleanerfish dances, or physical contact between cleaner and client. 相似文献
953.
Henrik Widegren Jonas Erjef?lt Magnus Korsgren Morgan Andersson Lennart Greiff 《Respiratory research》2008,9(1):15
Background
TNFα may contribute to the pathophysiology of airway inflammation. For example, we have recently shown that nasal administration of TNFα produces late phase co-appearance of granulocyte and plasma exudation markers on the mucosal surface. The objective of the present study was to examine indices of granulocyte presence and activity in response to intranasal TNFα challenge.Methods
Healthy subjects and patients with allergic rhinitis (examined out of season) were subjected to nasal challenge with TNFα (10 μg) in a sham-controlled and crossover design. Nasal lavages were carried out prior to and 24 hours post challenge. Nasal biopsies were obtained post challenge. Nasal lavage fluid levels of myeloperoxidase (MPO) and eosinophil cationic protein (ECP) were analyzed as indices of neutrophil and eosinophil activity. Moreover, IL-8 and α2-macroglobulin were analyzed as markers of pro-inflammatory cytokine production and plasma exudation. Nasal biopsy numbers of neutrophils and eosinophils were monitored.Results
Nasal lavage fluid levels of MPO recorded 24 hours post TNFα challenge were increased in healthy subjects (p = 0.0081) and in patients with allergic rhinitis (p = 0.0081) (c.f. sham challenge). Similarly, α2-macroglobulin was increased in healthy subjects (p = 0.014) and in patients with allergic rhinitis (p = 0.0034). Lavage fluid levels of ECP and IL-8 were not affected by TNFα challenge. TNFα increased the numbers of subepithelial neutrophils (p = 0.0021), but not the numbers of eosinophils.Conclusion
TNFα produces a nasal inflammatory response in humans that is characterised by late phase (i.e., 24 hours post challenge) neutrophil activity and plasma exudation. 相似文献954.
Background
Comparative sequencing studies among a wide range of taxonomic groups, including fungi, have led to the discovery that reproductive genes evolve more rapidly than other genes. However, for fungal reproductive genes the question has remained whether the rapid evolution is a result of stochastic or deterministic processes. The mating-type (mat) genes constitute the master regulators of sexual reproduction in filamentous ascomycetes and here we present a study of the molecular evolution of the four mat -genes (mat a-1, mat A-1, mat A-2 and mat A-3) of 20 Neurospora taxa. 相似文献955.
Sara Folkesson Hansson Maja Puchades Kaj Blennow Magnus Sj?gren Pia Davidsson 《Proteome science》2004,2(1):7
Background
The aim of this study was firstly, to improve and validate a cerebrospinal fluid (CSF) prefractionation method followed by two-dimensional electrophoresis (2-DE) and secondly, using this strategy to investigate differences between the CSF proteome of frontotemporal dementia (FTD) patients and controls. From each subject three ml of CSF was prefractionated using liquid phase isoelectric focusing prior to 2-DE. 相似文献956.
Comparative sequencing studies among a wide range of taxonomic groups, including fungi, provide the overall pattern that reproductive genes evolve more rapidly than other genes, and this divergence is believed to be important in the establishment of reproductive barriers between species. In this study, we investigated the molecular evolution of the pheromone receptor genes pre-1 and pre-2 of strains belonging to 12 and 13 heterothallic taxa, respectively, of the model genus Neurospora. Furthermore, we examined the regulatory pattern of both pheromone precursor and receptor genes during sexual crosses of Neurospora crassa and Neurospora intermedia, for which reinforcement of interspecific reproductive barriers in sympatry previously has been documented. We conclude that the part encoding the C-terminal intracellular domain of pre-1 and pre-2 genes evolves rapidly. Both stochastic and directional processes drive this divergence; both genes contain neutrally evolving codons, and in addition, pre-1 contains codons evolving under positive selection, whereas in pre-2 we found highly variable regions with numerous repeats encoding glycine, threonine, or aspartic acid. In addition, we found regulatory changes of the pheromone and receptor genes during crosses between N. crassa and N. intermedia with different reproductive success. Gene expression levels are higher in the interspecific sympatric crosses with low reproductive success than in their intraspecific and/or allopatric equivalents, both at the stage of initial communication and contact and later at postfertilization stages. Taken together, our data indicate that pheromones and receptors are important key players during reproductive isolation between Neurospora species, and this study provides a general framework for future studies on the role of reproductive proteins for reproductive isolation. 相似文献
957.
Calcium-induced generation of reactive oxygen species in brain mitochondria is mediated by permeability transition 总被引:1,自引:0,他引:1
Hansson MJ Månsson R Morota S Uchino H Kallur T Sumi T Ishii N Shimazu M Keep MF Jegorov A Elmér E 《Free radical biology & medicine》2008,45(3):284-294
Mitochondrial uptake of calcium in excitotoxicity is associated with subsequent increase in reactive oxygen species (ROS) generation and delayed cellular calcium deregulation in ischemic and neurodegenerative insults. The mechanisms linking mitochondrial calcium uptake and ROS production remain unknown but activation of the mitochondrial permeability transition (mPT) may be one such mechanism. In the present study, calcium increased ROS generation in isolated rodent brain and human liver mitochondria undergoing mPT despite an associated loss of membrane potential, NADH and respiration. Unspecific permeabilization of the inner mitochondrial membrane by alamethicin likewise increased ROS independently of calcium, and the ROS increase was further potentiated if NAD(H) was added to the system. Importantly, calcium per se did not induce a ROS increase unless mPT was triggered. Twenty-one cyclosporin A analogs were evaluated for inhibition of calcium-induced ROS and their efficacy clearly paralleled their potency of inhibiting mPT-mediated mitochondrial swelling. We conclude that while intact respiring mitochondria possess powerful antioxidant capability, mPT induces a dysregulated oxidative state with loss of GSH- and NADPH-dependent ROS detoxification. We propose that mPT is a significant cause of pathological ROS generation in excitotoxic cell death. 相似文献
958.
Berglund M Dalence-Guzmán MF Skogvall S Sterner O 《Bioorganic & medicinal chemistry》2008,16(5):2513-2528
Capsazepine as well as its derivatives and analogues are general inhibitors of constriction of human small airways. From a systematic variation of the capsazepine structure, divided into four regions, SARs were established. This paper concerns the chlorination of the A-ring as well as the replacement of the catechol with bioisosteric groups. It is revealed that chlorination of the A-ring has a profound effect on activity. Moreover, di-chlorination of the 6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline structure results in a 10-fold increase in potency compared to capsazepine. 相似文献
959.
We made a quantitative analysis of the responses of urban and rural residents in Sweden to arguments supporting and opposing
conservation of large carnivores. The most important arguments in favour of conservation were: “I want them [the large carnivores] to exist in Sweden, even if I will never see any of them”, “Sweden should share the responsibility of conserving the large
carnivores” and “We owe it [conservation of large carnivores] to future generations”. We found only small differences between rural and urban residents. For arguments opposing conservation,
the difference between rural and urban areas was slightly greater. The most important arguments opposing conservation of large
carnivores were: “They may have serious negative impact on livestock farming”, “They may have serious negative impact on reindeer
husbandry” and “May inflict suffering on injured livestock”. We conclude that there seems to be less support for direct use
values such as hunting, ecotourism or just experiencing large carnivores, this may imply that the minimum viable population
size can be used as a long-term management goal for large carnivore populations, possibly with an exception for bears. We
also conclude that a separate conservation or management plan is needed for each species, since the conflicts with human interests
vary greatly between the different carnivore species. 相似文献
960.
Studies of whether disturbance events are associated with the changing genetic compositions of natural populations may provide
insights into the importance of local selection events in maintaining diversity, and might inform plans for the conservation
and protection of that diversity. We examined the dynamics of a colour pattern polymorphism in a natural population of pygmy
grasshoppers Tetrix subulata (Orthoptera: Tetrigidae) inhabiting a previously burnt clear-cut area. Data on morph frequencies for wild-caught and captive-reared
individuals indicated that the initial dominance of black phenotypes following the fire event was followed by an increased
diversity of the polymorphism. This was manifested as the appearance of a novel morph, a decreased incidence of the black
morph, and a more even distribution of individuals across alternative morphs following the recurrence of vegetation. We also
found that the colour patterns of captive-reared individuals resembled those of their parents and that the degree of within-clutch
diversity increased between generations. Our comparisons of morph frequencies across generations and between environments
within generations point to a genetic determination of colour pattern, and indicate that the polymorphism is influenced more
strongly by selection than by plasticity or migration. 相似文献