全文获取类型
收费全文 | 3474篇 |
免费 | 258篇 |
出版年
2023年 | 16篇 |
2022年 | 39篇 |
2021年 | 96篇 |
2020年 | 43篇 |
2019年 | 76篇 |
2018年 | 111篇 |
2017年 | 118篇 |
2016年 | 167篇 |
2015年 | 176篇 |
2014年 | 228篇 |
2013年 | 254篇 |
2012年 | 295篇 |
2011年 | 317篇 |
2010年 | 208篇 |
2009年 | 125篇 |
2008年 | 237篇 |
2007年 | 204篇 |
2006年 | 171篇 |
2005年 | 182篇 |
2004年 | 127篇 |
2003年 | 135篇 |
2002年 | 92篇 |
2001年 | 22篇 |
2000年 | 23篇 |
1999年 | 20篇 |
1998年 | 23篇 |
1997年 | 22篇 |
1996年 | 9篇 |
1995年 | 15篇 |
1994年 | 18篇 |
1993年 | 15篇 |
1992年 | 19篇 |
1991年 | 11篇 |
1990年 | 11篇 |
1989年 | 10篇 |
1988年 | 13篇 |
1987年 | 8篇 |
1985年 | 4篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 10篇 |
1980年 | 7篇 |
1979年 | 6篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 4篇 |
1971年 | 2篇 |
1968年 | 3篇 |
1967年 | 3篇 |
1954年 | 2篇 |
排序方式: 共有3732条查询结果,搜索用时 31 毫秒
21.
22.
23.
24.
Jan Lindgren Magdalena Blaszczyk Barbara Atkinson Zenon Steplewski Hilary Koprowski 《Cancer immunology, immunotherapy : CII》1986,22(1):1-7
Summary Over 600 hybridomas were derived from the immunization of mice with live cells and aqueous extracts of the human prostatic carcinoma cell line PC3. A total of 26 hybridomas with restricted reactivities were selected, subcloned and antibodies tested on a variety of tumor and normal cells. Seven monoclonal antibodies showed reactivity for prostate cancer and other tumor cell lines, including breast carcinomas. Three of the antibodies obtained after immunization with live cells reacted with live cells only and three of the four antibodies obtained after immunization with cell extract reacted with cell extracts and spent culture media. The fourth antibody in the latter group was reactive only in the immunoperoxidase staining assay. Antibody PrS5 recognized a 90,000 molecular weight molecule from 125I-surface-labeled cells in immunoprecipitation analysis. Antibodies PrE3 and PrD8 detected a nonacid glycolipid pentasaccharide from PC3 cells and meconium, and a glycoprotein of 115,000 molecular weight from 125I-surface-labeled red blood cells. The similar patterns of reactivity in RIAs and antigen analysis suggest that antibodies PrE3 and PrD8 recognize the same molecule. The results emphasize the usefulness of immunohistochemistry in the testing of monoclonal antibodies and the impact of the form in which the antigen is presented on the resultant antibody specificity 相似文献
25.
A protein binds the selenocysteine insertion element in the 3'-UTR of mammalian selenoprotein mRNAs. 总被引:6,自引:0,他引:6
下载免费PDF全文
![点击此处可从《Nucleic acids research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Several gene products are involved in co-translational insertion of selenocysteine by the tRNA(Sec). In addition, a stem-loop structure in the mRNAs coding for selenoproteins is essential to mediate the selection of the proper selenocysteine UGA codon. Interestingly, in eukaryotic selenoprotein mRNAs, this stem-loop structure, the selenocysteine insertion sequence (SECIS) element, resides in the 3'-untranslated region, far downstream of the UGA codon. In view of unravelling the underlying complex mechanism, we have attempted to detect RNA-binding proteins with specificity for the SECIS element. Using mobility shift assays, we could show that a protein, present in different types of mammalian cell extracts, possesses the capacity of binding the SECIS element of the selenoprotein glutathione peroxidase (GPx) mRNA. We have termed this protein SBP, for Secis Binding Protein. Competition experiments attested that the binding is highly specific and UV cross-linking indicated that the protein has an apparent molecular weight in the range of 60-65 kDa. Finally, some data suggest that the SECIS elements in the mRNAs of GPx and another selenoprotein, type I iodothyronine 5' deiodinase, recognize the same SBP protein. This constitutes the first report of the existence of a 3' UTR binding protein possibly involved in the eukaryotic selenocysteine insertion mechanism. 相似文献
26.
Barbara Huisamen Elna Ellis Magdalena van Dyk Amanda Lochner 《Molecular and cellular biochemistry》1996,162(1):1-9
Although it is well-accepted that the phosphatidylinositol signalling transduction pathway, producing inositol-1,4,5-P3 (InsP3) and inositol-1,3,4,5-P4 (InsP4) as second messengers, functions in heart muscle, virtually nothing is known about the roles of the higher inositol polyphosphates such as inositolhexakisphosphate (InsP6). This study demonstrates that InSP6 has the ability to bind intracellularly, with different binding characteristics, to different myocardial membranes. Binding to purified sarcoplasmic reticulum (SR) membranes, purified sarcolemmal (SL) membranes as well as to viable mitochondria were characterized. Binding to all these membranes display high as well as low affinity binding sites, with differing affinities. Kd values of binding to SR were 32 and 383 nM, to SL 61 and 1312 nM, while those of mitochondrial binding were 230 and 2200 nM respectively.InsP4 binding was also investigated and displayed the following characteristics: to SR, one low affinity binding site (Kd = 203 nM) and to SL, a high as well as a low affinity binding site with Kd values of 41 and 2075 nM respectively. Presence of InsP3, the second messenger for SR calcium release, at concentrations of 1 nM, elevated the binding of InsP4 to SR and SL by a mean of 30% and 20% respectively.Fractionation of SR and SL membranes on sucrose density gradients, after solubilization with CHAPS, indicated that InsP6 bound to two separate protein peaks in both these membranes, while InsP4 bound to only one. In SR membranes, InsP4 bound preferentially to a protein separating at high sucrose density while it bound to a protein separating at low sucrose density in SL membranes. 相似文献
27.
Changes in in vivo fluorescence quenching in rye and barley as a function of reduced PSII light harvesting antenna size 总被引:1,自引:0,他引:1
Stefan Falk Marianna Krol Denis P. Maxwell David A. Rezansoff Gordon R. Gray Norman P. A. Huner 《Physiologia plantarum》1994,91(4):551-558
The relationship between the size of the light harvesting antenna to photosystem II (LHCII) and quenching of non-photochemical and dark level fluorescence was studied in wild-type rye (Secale cereale L. cv. Musketeer) and barley (Hordeum vulgare L. cv. Gunilla) as well as in the barley chlorophyll b-less chlorina F2 mutant (H. vulgare L. cv. Dornaria, chlorina-F2). Exposure for 10 min to an irradiance of 500 μmol m?2 s?1 resulted in a strong (0.71–0.73) non-photochemical (qs) quenching of the fluorescence yield in wild-type (WT) material, while the barley chlorina F2-mutant was quenched to 75% of this level. Relaxation of qs in darkness revealed a fast initial decay, related to relaxation of the high-energy-state dependent (qE) part of qs. Etiolated seedlings of rye and barley exposed to intermittent light (IML) for 36 cycles of 2 min light and 118 min darkness had suppressed Chl b and LHCII-production in both WT rye and barley, while the barley chlorina F2-mutant became totally devoid of all LHCII-polypeptides. It was found that the levels of qs and qs were similar in control grown barley chlorina F2 and IML-grown WT rye and barley, but qs was reduced by 30 to 35% and qs by 50 to 65%, respectively, as compared to control-grown. WT plants. No significant qs could be detected in IML-grown barley chlorina F2. It is clear, from these changes in in vivo fluorescence quenching in rye and barley that a significant level of qs is detectable even in the absence of LHCII. Only when the proximal antennae are totally absent, does qE completely disappear. We conclude that the presence of LHCII is not an absolute requirement for qE-quenching and suggest that distal as well as proximal antenna may contribute to qE in vivo. 相似文献
28.
Non-motile mutants of Helicobacter pylori and Helicobacter mustelae defective in flagellar hook production 总被引:6,自引:0,他引:6
Flagellar hooks were purified from Helicobacter pylori and Helicobacter mustelae. The 70 × 16nm H. pylori hook was composed of FIgE subunits of 78kDa, while the 72 × 16nm H. mustelae hook was composed of 87kDa subunits. N-terminal sequence was obtained for the FIgH proteins of both species, and for an internal H. mustelae FlgE peptide. Degenerate oligonucleotide primers allowed amplification of a 1.2 kb fragment from the H. mustelae chromosome, which carried part of the flgE gene. The corresponding H. pylori gene was cloned by immunoscreening of a genomic library constructed in λZAP Express, The translated H. pylori flgE sequence indicated a protein with limited homology with the hook proteins from Salmonella typhimurium and Treponema phagedenis. Mutants of H. pylori and H. mustelae defective in hook production generated by allele replacement were non-motile and devoid of flagellar filaments but produced both flagellin subunits, which were localized in the soluble fraction of the cell. The level of flagellin production was unchanged in the mutants, indicating that the regulation of flagellin expression in Helicobacter differs from that in the Enterobacteriaceae. 相似文献
29.
30.
Adrianna Nowicka Magdalena Kanabus Ewa Sledziewska-Gójska Zygmunt Ciesla 《Molecular genetics and genomics : MGG》1994,243(5):584-592
An Escherichia coli strain bearing the dnaQ49 mutation, which results in a defective s subunit of DNA polymerase III, and carrying the lexA71 mutation, which causes derepression of the SOS regulon, is totally unable to maintain high-copy-number plasmids containing the umuDC operon. The strain is also unable to maintain the pAN4 plasmid containing a partial deletion of the umuD gene but retaining the wild-type umuC gene. These results suggest that a high cellular level of UmuC is exceptionally harmful to the defective DNA polymerase III of the dnaQ49 mutant. We have used this finding as a basis for selection of new plasmid umuC mutants. The properties of two such mutants, bearing the umuC61 or umuC95 mutation, are described in detail. In the umuC122:: Tn 5 strain harbouring the mutant plasmids, UV-induced mutagenesis is severely decreased compared to that observed with the parental umuDC + plasmid. Interestingly, while the frequency of UV-induced GC → AT transitions is greatly reduced, the frequency of AT → TA transversions is not affected. Both mutant plasmids bear frameshift mutations within the same run of seven A residues present in umuC +; in umuC61 the run is shortened to six A whereas in umuC95 is lengthened to eight A. We have found in both umuC61 and umuC95 that translation is partially restored to the proper reading frame. We propose that under conditions of limiting amounts of UmuC, the protein preferentially facilitates processing of only some kinds of UV-induced lesions. 相似文献