Rapid measurement of free amino acids in serum and CSF using high-performance liquid chromatography

A simple, rapid method for measuring individual amino acids in physiological fluids using high-performance liquid chromatography [HPLC]/fluorescence detection has been characterized. Solutions containing free amino acids are first derivatized with orthophthalaldehyde, and then chromatographed on C₁₈ reversed-phase columns. Sixteen amino acids are readily separated and quantitated, with a run time of about 60 min, and a sensitivity of at least 10 pmoles. Applying this technique to solutions of amino acid standards, and methanol supernatants of a large pool of blood, we find within-assay and between-assay coefficients of variation generally to be as good as those obtained using a standard amino acid analyzer. Serum amino acid measurements have also been obtained under a number of experimental conditions in rats, and yielded values comparable to those obtained using the amino acid analyzer. Samples of cerebrospinal fluid have also been analyzed via this new procedure. This technique offers numerous advantages over the oftentimes laborious, expensive, and time-consuming procedures required by conventional amino acid analyzers, and thus should find widespread application.     

Isotope tracing in adult zebrafish reveals alanine cycling between melanoma and liver

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Large‐scale molecular barcoding of prey DNA reveals predictors of intrapopulation feeding diversity in a marine predator

Predator–prey interactions are critical in understanding how communities function. However, we need to describe intraspecific variation in diet to accurately depict those interactions. Harbor seals (Phoca vitulina) are an abundant marine predator that prey on species of conservation concern. We estimated intrapopulation feeding diversity (variation in feeding habits between individuals of the same species) of harbor seals in the Salish Sea. Estimates of feeding diversity were examined relative to sex, month, and location using a novel approach that combined molecular techniques, repeated cross‐sectional sampling of scat, and a specialization metric (within‐individual consistency in diet measured by the Proportional Similarity Index ()). Based on 1,083 scat samples collected from five haul‐out sites during four nonsequential years, we quantified diet using metabarcoding techniques and determined the sex of the scat depositor using a molecular assay. Results suggest that intrapopulation feeding diversity was present. Specialization was high over short periods (24–48 hr,  = 0.392, 95% CI = 0.013, R = 100,000) and variable in time and space. Females showed more specialization than males, particularly during summer and fall. Additionally, demersal and benthic prey species were correlated with more specialized diets. The latter finding suggests that this type of prey likely requires specific foraging strategies and that there are trade‐offs between pelagic and benthic foraging styles for harbor seals. This differential feeding on prey species, as well as between sexes of harbor seals, indicates that predator–prey interactions in harbor seals are complex and that each sex may have a different impact on species of conservation concern. As such, describing intrapopulation feeding diversity may unravel hitherto unknown complex predator–prey interactions in the community.