全文获取类型
收费全文 | 897篇 |
免费 | 62篇 |
专业分类
959篇 |
出版年
2023年 | 10篇 |
2022年 | 16篇 |
2021年 | 27篇 |
2020年 | 19篇 |
2019年 | 19篇 |
2018年 | 15篇 |
2017年 | 15篇 |
2016年 | 25篇 |
2015年 | 42篇 |
2014年 | 45篇 |
2013年 | 61篇 |
2012年 | 61篇 |
2011年 | 58篇 |
2010年 | 35篇 |
2009年 | 29篇 |
2008年 | 42篇 |
2007年 | 42篇 |
2006年 | 34篇 |
2005年 | 39篇 |
2004年 | 38篇 |
2003年 | 25篇 |
2002年 | 23篇 |
2001年 | 6篇 |
2000年 | 6篇 |
1999年 | 6篇 |
1998年 | 13篇 |
1996年 | 6篇 |
1995年 | 7篇 |
1994年 | 5篇 |
1993年 | 10篇 |
1992年 | 8篇 |
1991年 | 11篇 |
1990年 | 4篇 |
1989年 | 5篇 |
1988年 | 7篇 |
1987年 | 7篇 |
1986年 | 7篇 |
1983年 | 6篇 |
1982年 | 13篇 |
1981年 | 8篇 |
1980年 | 9篇 |
1978年 | 9篇 |
1976年 | 4篇 |
1974年 | 6篇 |
1973年 | 6篇 |
1972年 | 6篇 |
1971年 | 7篇 |
1970年 | 5篇 |
1969年 | 9篇 |
1968年 | 7篇 |
排序方式: 共有959条查询结果,搜索用时 15 毫秒
101.
Plant productivity is predicted to increase in northern latitudes as a result of climate warming; however, this may depend on whether biological nitrogen (N)-fixation also increases. We evaluated how the variation in temperature and light affects N-fixation by two boreal feather mosses, Pleurozium schreberi and Hylocomium splendens, which are the primary source of N-fixation in most boreal environments. We measured N-fixation rates 2 and 4 wk after exposure to a factorial combination of environments of normal, intermediate and high temperature (16.3, 22.0 and 30.3°C) and light (148.0, 295.7 and 517.3 μmol m(-2) s(-1)). Our results showed that P. schreberi achieved higher N-fixation rates relative to H. splendens in response to warming treatments, but that the highest warming treatment eventually caused N-fixation to decline for both species. Light strongly interacted with warming treatments, having positive effects at low or intermediate temperatures and damaging effects at high temperatures. These results suggest that climate warming may increase N-fixation in boreal forests, but that increased shading by the forest canopy or the occurrence of extreme temperature events could limit increases. They also suggest that P. schreberi may become a larger source of N in boreal forests relative to H. splendens as climate warming progresses. 相似文献
102.
Naveen Parmar Kyle Burrows Pia M. Vornewald Hvard T. Lindholm Rosalie T. Zwiggelaar Alberto Díez-Snchez Mara Martín-Alonso Madeleine Fosslie Bruce A. Vallance John Arne Dahl Colby Zaph Menno J. Oudhoff 《PLoS pathogens》2021,17(3)
Infectious and inflammatory diseases in the intestine remain a serious threat for patients world-wide. Reprogramming of the intestinal epithelium towards a protective effector state is important to manage inflammation and immunity and can be therapeutically targeted. The role of epigenetic regulatory enzymes within these processes is not yet defined. Here, we use a mouse model that has an intestinal-epithelial specific deletion of the histone demethylase Lsd1 (cKO mice), which maintains the epithelium in a fixed reparative state. Challenge of cKO mice with bacteria-induced colitis or a helminth infection model both resulted in increased pathogenesis. Mechanistically, we discovered that LSD1 is important for goblet cell maturation and goblet-cell effector molecules such as RELMß. We propose that this may be in part mediated by directly controlling genes that facilitate cytoskeletal organization, which is important in goblet cell biology. This study therefore identifies intestinal-epithelial epigenetic regulation by LSD1 as a critical element in host protection from infection. 相似文献
103.
Justin Maire Sam K. Girvan Sophie E. Barkla Alexis Perez-Gonzalez David J. Suggett Linda L. Blackall Madeleine J. H. van Oppen 《The ISME journal》2021,15(7):2168
Corals house a variety of microorganisms which they depend on for their survival, including endosymbiotic dinoflagellates (Symbiodiniaceae) and bacteria. While cnidarian–microorganism interactions are widely studied, Symbiodiniaceae–bacteria interactions are only just beginning to receive attention. Here, we describe the localization and composition of the bacterial communities associated with cultures of 11 Symbiodiniaceae strains from nine species and six genera. Three-dimensional confocal laser scanning and electron microscopy revealed bacteria are present inside the Symbiodiniaceae cells as well as closely associated with their external cell surface. Bacterial pure cultures and 16S rRNA gene metabarcoding from Symbiodiniaceae cultures highlighted distinct and highly diverse bacterial communities occur intracellularly, closely associated with the Symbiodiniaceae outer cell surface and loosely associated (i.e., in the surrounding culture media). The intracellular bacteria are highly conserved across Symbiodiniaceae species, suggesting they may be involved in Symbiodiniaceae physiology. Our findings provide unique new insights into the biology of Symbiodiniaceae.Subject terms: Symbiosis, Microbiome, Marine microbiology 相似文献
104.
105.
Lejour M 《Plastic and reconstructive surgery》2007,120(7):2106-2109
106.
Spatial dependency of paraspinal muscle activity was assessed using a new two-dimensional MMG recording system. MMG signals were detected over the left and right paraspinal muscles of 10 volunteers using a grid of 12 accelerometers. During two separate trials subjects maintained a 20 degrees flexed position and held loads that ranged from 0 to 15 kg (in 2.5 kg increments) for 20s; and 7.5 kg for 6 min. Maps of absolute and normalised (with respect to initial values) average rectified value, mean power frequency, variance and skewness of the power spectral density were obtained from the two-dimensional MMG recordings. For both the short duration and sustained contractions, the MMG absolute average rectified value, mean power frequency, variance and skewness depended on accelerometer location (P<0.05), while, with the exception of the skewness (P<0.05), normalised values did not. These results demonstrate both inhomogeneous MMG absolute activity and homogeneous MMG normalised activity in paraspinal muscles for short duration and sustained contractions. Moreover, the effect of accelerometer location on spectral variables confirmed the limited validity of general relationships between MMG spectral changes and motor unit recruitment strategies. This study underlines the importance of using multiple recording sites when assessing back muscle activity. 相似文献
107.
Ward Delphi Melbourne-Thomas Jessica Pecl Gretta T. Evans Karen Green Madeline McCormack Phillipa C. Novaglio Camilla Trebilco Rowan Bax Narissa Brasier Madeleine J. Cavan Emma L. Edgar Graham Hunt Heather L. Jansen Jan Jones Russ Lea Mary-Anne Makomere Reuben Mull Chris Semmens Jayson M. Shaw Janette Tinch Dugald van Steveninck Tatiana J. Layton Cayne 《Reviews in Fish Biology and Fisheries》2022,32(1):65-100
Reviews in Fish Biology and Fisheries - Marine ecosystems and their associated biodiversity sustain life on Earth and hold intrinsic value. Critical marine ecosystem services include maintenance of... 相似文献
108.
109.
Barta TE Becker DP Bedell LJ Easton AM Hockerman SL Kiefer J Munie GE Mathis KJ Li MH Rico JG Villamil CI Williams JM 《Bioorganic & medicinal chemistry letters》2011,21(10):2820-2822
Seeking compounds preferentially potent and selective for MMP-13, we reported in the preceding Letter on a series of hydroxamic acids with a flexible benzamide tail groups.1a Here, we replace the amide moiety with non-hydrolyzable heterocycles in an effort to improve half-life. We identify a hydroxamate tetrazole 4e that spares MMP-1 and -14, shows >400-fold selectivity versus MMP-8 and >600-fold selectivity versus MMP-2, and has a 4.8 h half-life in rats. X-ray data (1.9 Å) for tetrazole 4c is presented. 相似文献
110.
Effective management of reef corals requires knowledge of the extent to which populations are open or closed and the scales over which genetic exchange occurs, information which is commonly derived from population genetic data. Such data are sparse for Great Barrier Reef (GBR) corals and other organisms, with the studies that are available being mostly based on a small number of sampling locations spanning only part of the GBR. Using 11 microsatellite loci, we genotyped 947 colonies of the reef-building coral Acropora millepora from 20 sites spanning almost the full length of the GBR (~12° of latitude and ~1550 km). The results show a major divide between the southernmost central to southern offshore populations and all other sampled populations. We interpret this divide as a signature of allopatric divergence in northern and southern refugia during the Pleistocene glaciations, from which the GBR was subsequently recolonized. Superimposed on this pattern is a cross-shelf genetic division, as well as a separation of inshore populations south of the Cape Clifton Front at ~21.5-22°S. Most inshore populations north of this, as well as mid-shelf populations in the northern and far northern GBR, are open, exchanging recruits frequently. In contrast, inshore populations south of the Cape Clifton Front and offshore populations in the central and southern GBR are largely self-seeding, at least within the spatial resolution that was achieved given our sampling intensity. Populations that have been impacted by recent disturbance events causing extensive coral mortality show no evidence of reduced genetic diversity. 相似文献