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991.
992.
In a previous laboratory study, larvae of the ant-tended lycaenid butterfly Hemiargus isola developed into larger adults when reared with the ant Formica perpilosa than when reared without ants. Ants neither fed butterfly larvae nor significantly delayed developmental duration. We investigated
two non-exclusive hypotheses for the mechanism of this effect: larvae tended by F. perpilosa (1) consume more food, and (2) digest the food they consume more efficiently, than those reared without ants. Larvae reared
in the laboratory with F. perpilosa ants became significantly heavier adults but produced a significantly lighter fecal mass than their untended counterparts,
suggesting that greater food consumption was not the primary mechanism for the higher growth rates of ant-tended larvae. Tended
and untended larvae were equally proficient at digesting the contents of pollen (a major natural food source) throughout the
tended portion of the life cycle. Taken together, the results suggest that neither greater consumption nor higher assimilation
accounts for the larger size of F. perpilosa-tended larvae. We propose that tended larvae may expend less energy than their untended counterparts.
Received: 3 January 1997 / Accepted: 18 June 1997 相似文献
993.
A. H. del Rio J. B. Bamberg Z. Huaman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):191-198
Effects of gene bank seed-increases on the genetic integrity of potato germ plasm is a major concern of gene bank managers.
Thus the Association of Potato Inter-gene-bank Collaborators (APIC), a consortium of world potato gene bank leaders, initiated
this joint research project using RAPD markers to determine genetic relationships between increased generations within accessions.
Solanum jamesii (2n=2x=24) and S. fendleri (2n=4x=48), two wild potato species native to North America, were used as plant material. These species represented two major breeding
systems found among Solanum species: outcrossing diploids and inbreeding disomic tetraploids, respectively. Comparisons were made between populations
one generation apart and between sister populations generated from a common source. Fourteen such comparisons within S. jamesii accessions had an average similarity of 96.3%, and 21 such comparisons within S. fendleri accessions had an average similarity of 96.0%. No pairs of populations were significantly different, despite the fact that
RAPD markers easily separated all of these very similar accessions within their respective species. Only one of six S. jamesii accessions analyzed showed a significant change in gene frequencies among generations. These findings indicate that there
has been minimal loss or change of genetic diversity in ex situ germplasm using the gene bank techniques standard at NRSP-6 and other world potato gene banks.
Received: 28 October 1996 / Accepted: 7 March 1997 相似文献
994.
M. García A. Gradillas C. del Campo E.F. Llama J.M. Sanchez-Montero J.V. Sinisterra 《Biotechnology letters》1997,19(10):999-1004
sThe hydrolysis of several esters catalyzed by lipase of Candida rugosa was used to analyse the interaction between the ester substrate and the active site and the geometry and steric restrictions of alk, ar and h subsites. Using 4-R-C H -CH(R)-COOEt and ethyl trans-cinnamate, the presence of a low electronic density zone in the ar pocket is described, the dimensions of the pocket produced by Phe-296 and Leu-297 in the alk subsite and the size of the h subsite are described. 相似文献
995.
I. Faus C. Pati-o J.L. del R'o C. del Moral H.S. Barroso J. Bladé V. Rubio 《Biotechnology letters》1997,19(12):1185-1191
A synthetic gene encoding the amino acid sequence of the sweet-tasting protein thaumatin II has been expressed in Penicillium roquefortii. Using several different expression cassettes thaumatin was expressed either intracellularly or extracellularly, at concentrations of 1–2 mg thaumatin/l. 相似文献
996.
B. Büdel U. Becker S. Porembski W. Barthlott 《Plant biology (Stuttgart, Germany)》1997,110(6):458-465
This study focuses on the saxicolous lichens and cyanobacteria of the open, exposed rock surface of inselbergs. Twenty-three species of cyanobacteria and 17 cyanobacterial lichen species (“cyanolichens”) from several inselbergs and other rocky outcrops of three major climatic regions, savanna, transition zone and rain forest, are reported from the Ivory Coast. Inselbergs are isolated and frequently mountains consisting of Precambrian granites or gneisses that abruptly rise from the surrounding plains. Cyanobacteria were found to be the dominating organisms on all rock surfaces. The lichens found mainly belong to the family Peltulaceae and a few were present from the family Lichinaceae. Nine species of the cyanolichens and most of the cyanobacteria are new for the Ivory Coast. A gradient in total species number (cyanolichens and cyanobacteria) occurs from the savanna to the rain forest, with a decrease in species number towards the rain forest. Saxicolous cyanobacterial lichens reached a higher species number in the savanna type ecosystem (11) than on inselbergs in the rain forest (7). The cyanolichens and cyanobacteria found are characteristic for larger, light-exposed rock surfaces and species like P. congregate, P. lingulata, P. tortuosa and P. umbilicata preferentially occur on the granite or sandstone of inselbergs. 相似文献
997.
Raúl Cueva del Castillo J. Núez-Farfn 《Evolution; international journal of organic evolution》1999,53(1):209-215
We measured in the field the intensity and mode (i.e., directional, stabilizing) of sexual selection acting jointly on body size and time of sexual maturity in the univoltine, polygamous grasshopper Sphenarium purpurascens. Statistical analyses indicated that selection favored large and protandrous males in terms of a higher mating success. At the same time, evidence of correlational selection acting simultaneously on body size and time to sexual maturity was found. Thus, selection should strengthen the relationship between body size and the time of sexual maturity. Theoretical work suggests the existence of a trade-off between reaching a large size and early sexual maturation in insects. The present study does not support the existence of this kind of trade-off. Recent theoretical and empirical work like the one reported here suggests that such a trade-off may not be necessarily expected if growth rates (which are often assumed to be invariable) are affected by environmental and genetic factors. 相似文献
998.
999.
Araceli del Arco Julián Morcillo Juan Ramon Martínez-Morales Carmen Galián Vera Martos Paola Bovolenta Jorgina Satrústegui 《European journal of biochemistry》2002,269(13):3313-3320
Aralar1 and citrin are members of the subfamily of calcium-binding mitochondrial carriers and correspond to two isoforms of the mitochondrial aspartate/glutamate carrier (AGC). These proteins are activated by Ca2+ acting on the external side of the inner mitochondrial membrane. Although it is known that aralar1 is expressed mainly in skeletal muscle, heart and brain, whereas citrin is present in liver, kidney and heart, the precise tissue distribution of the two proteins in embryonic and adult tissues is largely unknown. We investigated the pattern of expression of aralar1 and citrin in murine embryonic and adult tissues at the mRNA and protein levels. In situ hybridization analysis indicates that both isoforms are expressed strongly in the branchial arches, dermomyotome, limb and tail buds at early embryonic stages. However, citrin was more abundant in the ectodermal components of these structures whereas aralarl had a predominantly mesenchymal localization. The strong expression of citrin in the liver was acquired postnatally, whereas the characteristic expression of aralar1 in skeletal muscle was detected at E18 and that in the heart began early in development (E11) and was preferentially localized to auricular myocardium in late embryonic stages. Aralar1 was also expressed in bone marrow, T-lymphocytes and macrophages, including Kupffer cells in the liver, indicating that this is the major AGC isoform present in the hematopoietic system. Both aralar1 and citrin were expressed in fetal gut and adult stomach, ovary, testis, and pancreas, but only aralar1 is enriched in lung and insulin-secreting beta cells. These results show that aralar1 is expressed in many more tissues than originally believed and is absent from hepatocytes, where citrin is the only AGC isoform present. This explains why citrin deficiency in humans (type II citrullinemia) only affects the liver and suggests that aralar1 may compensate for the lack of citrin in other tissues. 相似文献
1000.
I. Ma. Martín del Molino M. Ulloa R. Martínez-Carrasco P. Pérez 《Physiologia plantarum》1986,66(3):503-508
Three wheat cultivars ( Triticum aestivum L.), Splendeur, Hobbit and Maris Huntsman grown in pots were compared. Especially when compared to Splendeur, the flag leaf senesced most rapidly in Maris Huntsman, which presented the most rapid loss of moisture, chlorophyll and nitrogen. The uptake of exogenous nitrogen during the post-anthesis period was lower in the rapidly than in the slowly senescing variety. A higher concentration of free amino nitrogen in the flag leaf at a given sampling date was associated with a lower percentage decrease of soluble proteins at the following date. Acid proteinase activity in the flag leaf was inversely related to moisture percentage and free amino nitrogen level, but unrelated to the nitrogen loss of the flag leaf. Acid proteinase activity in the flag leaf was directly related to grain nitrogen percentage, but inversely related to grain yield. Grain yield was also directly related to the mean soluble protein content of the flag leaf through senescence. 相似文献