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Ewen J. Maclean 《BMJ (Clinical research ed.)》1907,2(2448):1618-1619
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Ewen J. Maclean 《BMJ (Clinical research ed.)》1907,1(2412):716-717
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The plant antimicrobial peptide MiAMP1 from Macadamia integrifolia and the yeast killer toxin peptide WmKT from Williopsis mrakii are structural homologues. Comparative studies of yeast mutants were performed to test their sensitivity to these two antimicrobial peptides. No differences in susceptibility to MiAMP1 were detected between wild-type and several WmKT-resistant mutant yeast strains. A yeast mutant MT1, resistant to MiAMP1 but unaffected in its susceptibility to plant defensins and hydrogen peroxide, also did not show enhanced tolerance towards WmKT. It is therefore probable that the Greek key beta-barrel structure shared by MiAMP1 and WmKT provides a robust structural framework ensuring stability for the two proteins but that the specific action of the peptides depends on other motifs. 相似文献
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Maclean JA Chen MA Wayne CM Bruce SR Rao M Meistrich ML Macleod C Wilkinson MF 《Cell》2005,120(3):369-382
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Diniz FM Maclean N Ogawa M Cintra IH Bentzen P 《Marine biotechnology (New York, N.Y.)》2005,7(5):462-473
Atlantic spiny lobsters support major fisheries in northeastern Brazilian waters and in the Caribbean Sea. To avoid reduction
in diversity and elimination of distinct stocks, understanding their population dynamics, including structuring of populations
and genetic diversity, is critical. We here explore the potential of using the hypervariable domain in the control region
of the mitochondrial DNA as a genetic marker to characterize population subdivision in spiny lobsters, using Panulirus argus as the species model. The primers designed on the neighboring conserved genes have amplified the entire control region (approx.
780 bases) of P. argus and other closely related species. Average nucleotide and haplotype diversity within P. argus were found to be high, and population structuring was hypothesized. The data suggest a division of P. argus into genetically different phylogeographic groups. The hypervariable domain seems to be useful for determining genetic differentiation
of geographically distinct stocks of P. argus and other Atlantic spiny lobsters. 相似文献
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