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排序方式: 共有1349条查询结果,搜索用时 281 毫秒
101.
Effects of litter traits,soil biota,and soil chemistry on soil carbon stocks at a common garden with 14 tree species 总被引:1,自引:0,他引:1
Kevin E. Mueller Sarah E. Hobbie Jon Chorover Peter B. Reich Nico Eisenhauer Michael J. Castellano Oliver A. Chadwick Tomasz Dobies Cynthia M. Hale Andrzej M. Jagodziński Izabela Kałucka Barbara Kieliszewska-Rokicka Jerzy Modrzyński Anna Rożen Maciej Skorupski Łukasz Sobczyk Małgorzata Stasińska Lidia K. Trocha January Weiner Anna Wierzbicka Jacek Oleksyn 《Biogeochemistry》2015,123(3):313-327
102.
Maciej Stolarski 《Biological Rhythm Research》2015,46(3):417-423
The aim of the present article is to determine whether recently shown positive relationships between self-reported emotional intelligence (EI) and morningess preference exist when EI is measured with an ability-based test. In two studies (study 1: N = 206 and study 2: N = 184), we applied two different morningness–eveningness questionnaires and a performance test of EI consisting of four dimensions (perception, understanding, assimilation, managing). The two studies provided similar results indicating that “night owls” have higher EI than “larks”; specifically, evening-oriented subjects showed greater ability in emotion perception and understanding, as compared to morning-oriented individuals. The obtained results were in line with hitherto existing research on chronotype and general intelligence. The results provide further evidence for a cognitive character of ability-based EI and accentuate its different nature when compared to self-reported EI. The main findings are discussed in terms of evolutionary theories of circadian preferences, EI and mental abilities. 相似文献
103.
Marta Kulik Anna?M. Goral Maciej Jasiński Paulina?M. Dominiak Joanna Trylska 《Biophysical journal》2015,108(3):655-665
Electrostatic interactions often play key roles in the recognition of small molecules by nucleic acids. An example is aminoglycoside antibiotics, which by binding to ribosomal RNA (rRNA) affect bacterial protein synthesis. These antibiotics remain one of the few valid treatments against hospital-acquired infections by Gram-negative bacteria. It is necessary to understand the amplitude of electrostatic interactions between aminoglycosides and their rRNA targets to introduce aminoglycoside modifications that would enhance their binding or to design new scaffolds. Here, we calculated the electrostatic energy of interactions and its per-ring contributions between aminoglycosides and their primary rRNA binding site. We applied either the methodology based on the exact potential multipole moment (EPMM) or classical molecular mechanics force field single-point partial charges with Coulomb formula. For EPMM, we first reconstructed the aspherical electron density of 12 aminoglycoside-RNA complexes from the atomic parameters deposited in the University at Buffalo Databank. The University at Buffalo Databank concept assumes transferability of electron density between atoms in chemically equivalent vicinities and allows reconstruction of the electron densities from experimental structural data. From the electron density, we then calculated the electrostatic energy of interaction using EPMM. Finally, we compared the two approaches. The calculated electrostatic interaction energies between various aminoglycosides and their binding sites correlate with experimentally obtained binding free energies. Based on the calculated energetic contributions of water molecules mediating the interactions between the antibiotic and rRNA, we suggest possible modifications that could enhance aminoglycoside binding affinity. 相似文献
104.
High cell density cultivation of Pseudomonas putida KT2440 using glucose without the need for oxygen enriched air supply
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105.
Francine Z Marques Simon PR Romaine Matthew Denniff James Eales John Dormer Ingrid M Garrelds Lukasz Wojnar Katarzyna Musialik Barbara Duda-Raszewska Bartlomiej Kiszka Magdalena Duda Brian J Morris Nilesh J Samani AH Jan Danser Pawel Bogdanski Ewa Zukowska-Szczechowska Fadi J Charchar Maciej Tomaszewski 《Molecular medicine (Cambridge, Mass.)》2015,21(1):739-748
MicroRNA-181a binds to the 3′ untranslated region of messenger RNA (mRNA) for renin, a rate-limiting enzyme of the renin-angiotensin system. Our objective was to determine whether this molecular interaction translates into a clinically meaningful effect on blood pressure and whether circulating miR-181a is a measurable proxy of blood pressure. In 200 human kidneys from the TRANScriptome of renaL humAn TissuE (TRANSLATE) study, renal miR-181a was the sole negative predictor of renin mRNA and a strong correlate of circulating miR-181a. Elevated miR-181a levels correlated positively with systolic and diastolic blood pressure in TRANSLATE, and this association was independent of circulating renin. The association between serum miR-181a and systolic blood pressure was replicated in 199 subjects from the Genetic Regulation of Arterial Pressure of Humans In the Community (GRAPHIC) study. Renal immunohistochemistry and in situ hybridization showed that colocalization of miR-181a and renin was most prominent in collecting ducts where renin is not released into the systemic circulation. Analysis of 69 human kidneys characterized by RNA sequencing revealed that miR-181a was associated with downregulation of four mitochondrial pathways and upregulation of 41 signaling cascades of adaptive immunity and inflammation. We conclude that renal miR-181a has pleiotropic effects on pathways relevant to blood pressure regulation and that circulating levels of miR-181a are both a measurable proxy of renal miR-181a expression and a novel biochemical correlate of blood pressure. 相似文献
106.
Zajdel P Marciniec K Maślankiewicz A Paluchowska MH Satała G Partyka A Jastrzębska-Więsek M Wróbel D Wesołowska A Duszyńska B Bojarski AJ Pawłowski M 《Bioorganic & medicinal chemistry》2011,19(22):6750-6759
Novel arene- and quinolinesulfonamides were synthesized using different solutions and a solid-support methodology, and were evaluated for their affinity for 5-HT(1A), 5-HT(2A), 5-HT(6), and 5-HT(7) receptors. Compound 54 (N-Ethyl-N-[4-(1,2,3,4,4a,5,6,7,8,8a-decahydroisoquinolin-2-yl)butyl]-8-quinolinesulfonamide) was identified as potent 5-HT(7) antagonist (K(i)=13 nM, K(B)=140 nM) with good selectivity over 5-HT(1A), 5-HT(2A), 5-HT(6) receptors. In the FST in mice, it reduced immobility in a manner similar to the selective 5-HT(7) antagonist SB-269970. 相似文献
107.
Byrtus H Obniska J Czopek A Kamiński K Pawłowski M 《Bioorganic & medicinal chemistry》2011,19(20):6149-6156
Synthesis, physicochemical and anticonvulsant properties of new N-Mannich bases derived from 5-cyclopropyl-5-phenyl- and 5-cyclopropyl-5-(4-chlorophenyl)-imidazolidine-2,4-diones have been described. Initial anticonvulsant screening was performed using intraperitoneal (ip.) maximal electroshock (MES) and subcutaneous pentylenetetrazole (scPTZ) seizure tests. The neurotoxicity was determined applying the rotarod test. The in vivo results in mice showed that all compounds were effective especially in the MES screen. The quantitative evaluation after oral administration in rats showed that the most active was 5-cyclopropyl-5-phenyl-imidazolidine-2,4-dione (1) with ED(50) values of 5.76 mg/kg (MES) and 57.31 mg/kg (scPTZ). This molecule was more potent than phenytoin and ethosuximide which were used as reference antiepileptic drugs. Additionally compound 1 with ED(50) of 26.06 mg/kg in psychomotor seizure test (6-Hz) in mice showed comparable activity to new generation anticonvulsant - levetiracetam. 相似文献
108.
Krastev DB Slabicki M Paszkowski-Rogacz M Hubner NC Junqueira M Shevchenko A Mann M Neugebauer KM Buchholz F 《Nature cell biology》2011,13(7):809-818
TP53 (tumour protein 53) is one of the most frequently mutated genes in human cancer and its role during cellular transformation has been studied extensively. However, the homeostatic functions of p53 are less well understood. Here, we explore the molecular dependency network of TP53 through an RNAi-mediated synthetic interaction screen employing two HCT116 isogenic cell lines and a genome-scale endoribonuclease-prepared short interfering RNA library. We identify a variety of TP53 synthetic interactions unmasking the complex connections of p53 to cellular physiology and growth control. Molecular dissection of the TP53 synthetic interaction with UNRIP indicates an enhanced dependency of TP53-negative cells on small nucleolar ribonucleoprotein (snoRNP) assembly. This dependency is mediated by the snoRNP chaperone gene NOLC1 (also known as NOPP140), which we identify as a physiological p53 target gene. This unanticipated function of TP53 in snoRNP assembly highlights the potential of RNAi-mediated synthetic interaction screens to dissect molecular pathways of tumour suppressor genes. 相似文献
109.
Milena Niemiec Maciej G?ogowski Dobromira Tyc-Szczepaniak Marek Wierzchowski Lucyna K?pka 《Reports of Practical Oncology and Radiotherapy》2011,16(2):49-53
Background and aim
Long-term survival of lung cancer patients with brain metastases (BM) is very rare. Our aim is to report the characteristics of patients who survived for at least three years after a BM diagnosis.Materials and methods
Nineteen lung cancer patients who had survived ≥3 years after a BM diagnosis were identified in our database. Seven (37%) had undergone whole-brain radiotherapy (WBRT) only, five (26%) BM surgery + WBRT, three (16%) BM surgery + WBRT + BM radiosurgery, and four (21%) no WBRT (one, surgery; one, radiosurgery; two, BM surgery + radiosurgery). Their characteristics were compared with historical data for 322 lung cancer patients with BM (control group, CG), who had received WBRT between 1986 and 1997.Results
Median survival from BM in long survivors group was 73 months (in CG – 4 months). Characteristics comparison: median age 55 vs. 58 (CG), p = 0.16; female sex 68% vs. 28% (CG), p = 0.003; RTOG/RPA class 1 – 75% vs. 13% (CG), p = 0.00001; adenocarcinoma histology 84% vs. 24% (CG), p < 0.00001; control of primary tumor 95% vs. 27% (CG), p < 0.00001; extracranial metastases 0 vs. 26% (CG), p = 0.01; single BM 63% vs. 9% (CG), p = 0.00001; surgery of BM 53% vs. 14% (CG), p = 0.00001.Conclusions
Beside prognostic factors already recognized as favorable in patients with BM, the adenocarcinoma histology and female sex were prevalent in long-term survivors of BM from lung cancer. 相似文献110.
Large-scale candidate gene analysis of HDL particle features 总被引:1,自引:0,他引:1
Kaess BM Tomaszewski M Braund PS Stark K Rafelt S Fischer M Hardwick R Nelson CP Debiec R Huber F Kremer W Kalbitzer HR Rose LM Chasman DI Hopewell J Clarke R Burton PR Tobin MD Hengstenberg C Samani NJ 《PloS one》2011,6(1):e14529