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101.
SNPs in the porcine <Emphasis Type="Italic">PPARGC1a</Emphasis> gene: Interbreed differences and their phenotypic effects 总被引:1,自引:0,他引:1
Stachowiak M Szydlowski M Cieslak J Switonski M 《Cellular & molecular biology letters》2007,12(2):231-239
Due to its function, the peroxisome proliferative activated receptor-γ, coactivator-1α (PPARGC1A) gene is a candidate in the search for genes that may affect production traits in the pig. The purpose of this study was
to screen for new SNPs in exon 8 of the porcine PPARGC1A gene and to test their possible association with production traits. Altogether 736 pigs representing five breeds Polish Landrace,
n=242; Polish Large White, n=192; Hampshire, n=27; Duroc, 21; Pietrain, n=12) and synthetic line 990 (n=242) were scanned
via SSCP assay. Four SNPs were found; two new ones: C/G (His338Gln) and G/A Thr359Thr), and two previously reported ones:
C/A (Arg369Arg) and T/A Cys430Ser). The missense T/A and C/G SNPs demonstrated pronounced interbreed variability in terms
of allele frequencies, including the exclusive presence of the C/G substitution in the Hampshire breed. The tested SNPs occurred
in five putative haplotypes, and their frequency also differed substantially between breeds. The association of the SNPs with
production traits was tested for G/A (Thr359Thr), C/A (Arg369Arg) and T/A (Cys430Ser) substitutions in Polish Large White,
Polish Landrace and line 990. The analysis revealed only breed-specific associations. The T/A (Cys430Ser) SNP was related
to the feed conversion ratio in the Polish Large White (P=0.02), and the silent G/A and C/A substitutions were respectively
associated with abdominal fat in line 990 and backfat thickness in Polish Landrace (P=0.04). The combined effects of the substitutions
were estimated as haplotype effects. Three significant contrasts between haplotypes were calculated, but the observed associations
were again only breed-specific. 相似文献
102.
Effects of litter traits,soil biota,and soil chemistry on soil carbon stocks at a common garden with 14 tree species 总被引:1,自引:0,他引:1
Kevin E. Mueller Sarah E. Hobbie Jon Chorover Peter B. Reich Nico Eisenhauer Michael J. Castellano Oliver A. Chadwick Tomasz Dobies Cynthia M. Hale Andrzej M. Jagodziński Izabela Kałucka Barbara Kieliszewska-Rokicka Jerzy Modrzyński Anna Rożen Maciej Skorupski Łukasz Sobczyk Małgorzata Stasińska Lidia K. Trocha January Weiner Anna Wierzbicka Jacek Oleksyn 《Biogeochemistry》2015,123(3):313-327
103.
Maciej Stolarski 《Biological Rhythm Research》2015,46(3):417-423
The aim of the present article is to determine whether recently shown positive relationships between self-reported emotional intelligence (EI) and morningess preference exist when EI is measured with an ability-based test. In two studies (study 1: N = 206 and study 2: N = 184), we applied two different morningness–eveningness questionnaires and a performance test of EI consisting of four dimensions (perception, understanding, assimilation, managing). The two studies provided similar results indicating that “night owls” have higher EI than “larks”; specifically, evening-oriented subjects showed greater ability in emotion perception and understanding, as compared to morning-oriented individuals. The obtained results were in line with hitherto existing research on chronotype and general intelligence. The results provide further evidence for a cognitive character of ability-based EI and accentuate its different nature when compared to self-reported EI. The main findings are discussed in terms of evolutionary theories of circadian preferences, EI and mental abilities. 相似文献
104.
High cell density cultivation of Pseudomonas putida KT2440 using glucose without the need for oxygen enriched air supply 下载免费PDF全文
105.
Francine Z Marques Simon PR Romaine Matthew Denniff James Eales John Dormer Ingrid M Garrelds Lukasz Wojnar Katarzyna Musialik Barbara Duda-Raszewska Bartlomiej Kiszka Magdalena Duda Brian J Morris Nilesh J Samani AH Jan Danser Pawel Bogdanski Ewa Zukowska-Szczechowska Fadi J Charchar Maciej Tomaszewski 《Molecular medicine (Cambridge, Mass.)》2015,21(1):739-748
MicroRNA-181a binds to the 3′ untranslated region of messenger RNA (mRNA) for renin, a rate-limiting enzyme of the renin-angiotensin system. Our objective was to determine whether this molecular interaction translates into a clinically meaningful effect on blood pressure and whether circulating miR-181a is a measurable proxy of blood pressure. In 200 human kidneys from the TRANScriptome of renaL humAn TissuE (TRANSLATE) study, renal miR-181a was the sole negative predictor of renin mRNA and a strong correlate of circulating miR-181a. Elevated miR-181a levels correlated positively with systolic and diastolic blood pressure in TRANSLATE, and this association was independent of circulating renin. The association between serum miR-181a and systolic blood pressure was replicated in 199 subjects from the Genetic Regulation of Arterial Pressure of Humans In the Community (GRAPHIC) study. Renal immunohistochemistry and in situ hybridization showed that colocalization of miR-181a and renin was most prominent in collecting ducts where renin is not released into the systemic circulation. Analysis of 69 human kidneys characterized by RNA sequencing revealed that miR-181a was associated with downregulation of four mitochondrial pathways and upregulation of 41 signaling cascades of adaptive immunity and inflammation. We conclude that renal miR-181a has pleiotropic effects on pathways relevant to blood pressure regulation and that circulating levels of miR-181a are both a measurable proxy of renal miR-181a expression and a novel biochemical correlate of blood pressure. 相似文献
106.
Zajdel P Marciniec K Maślankiewicz A Paluchowska MH Satała G Partyka A Jastrzębska-Więsek M Wróbel D Wesołowska A Duszyńska B Bojarski AJ Pawłowski M 《Bioorganic & medicinal chemistry》2011,19(22):6750-6759
Novel arene- and quinolinesulfonamides were synthesized using different solutions and a solid-support methodology, and were evaluated for their affinity for 5-HT(1A), 5-HT(2A), 5-HT(6), and 5-HT(7) receptors. Compound 54 (N-Ethyl-N-[4-(1,2,3,4,4a,5,6,7,8,8a-decahydroisoquinolin-2-yl)butyl]-8-quinolinesulfonamide) was identified as potent 5-HT(7) antagonist (K(i)=13 nM, K(B)=140 nM) with good selectivity over 5-HT(1A), 5-HT(2A), 5-HT(6) receptors. In the FST in mice, it reduced immobility in a manner similar to the selective 5-HT(7) antagonist SB-269970. 相似文献
107.
Byrtus H Obniska J Czopek A Kamiński K Pawłowski M 《Bioorganic & medicinal chemistry》2011,19(20):6149-6156
Synthesis, physicochemical and anticonvulsant properties of new N-Mannich bases derived from 5-cyclopropyl-5-phenyl- and 5-cyclopropyl-5-(4-chlorophenyl)-imidazolidine-2,4-diones have been described. Initial anticonvulsant screening was performed using intraperitoneal (ip.) maximal electroshock (MES) and subcutaneous pentylenetetrazole (scPTZ) seizure tests. The neurotoxicity was determined applying the rotarod test. The in vivo results in mice showed that all compounds were effective especially in the MES screen. The quantitative evaluation after oral administration in rats showed that the most active was 5-cyclopropyl-5-phenyl-imidazolidine-2,4-dione (1) with ED(50) values of 5.76 mg/kg (MES) and 57.31 mg/kg (scPTZ). This molecule was more potent than phenytoin and ethosuximide which were used as reference antiepileptic drugs. Additionally compound 1 with ED(50) of 26.06 mg/kg in psychomotor seizure test (6-Hz) in mice showed comparable activity to new generation anticonvulsant - levetiracetam. 相似文献
108.
Krastev DB Slabicki M Paszkowski-Rogacz M Hubner NC Junqueira M Shevchenko A Mann M Neugebauer KM Buchholz F 《Nature cell biology》2011,13(7):809-818
TP53 (tumour protein 53) is one of the most frequently mutated genes in human cancer and its role during cellular transformation has been studied extensively. However, the homeostatic functions of p53 are less well understood. Here, we explore the molecular dependency network of TP53 through an RNAi-mediated synthetic interaction screen employing two HCT116 isogenic cell lines and a genome-scale endoribonuclease-prepared short interfering RNA library. We identify a variety of TP53 synthetic interactions unmasking the complex connections of p53 to cellular physiology and growth control. Molecular dissection of the TP53 synthetic interaction with UNRIP indicates an enhanced dependency of TP53-negative cells on small nucleolar ribonucleoprotein (snoRNP) assembly. This dependency is mediated by the snoRNP chaperone gene NOLC1 (also known as NOPP140), which we identify as a physiological p53 target gene. This unanticipated function of TP53 in snoRNP assembly highlights the potential of RNAi-mediated synthetic interaction screens to dissect molecular pathways of tumour suppressor genes. 相似文献
109.
Kroon AA Wang J Kavanagh BP Kavanagh B Huang Z Kuliszewski M van Goudoever JB Post M 《PloS one》2011,6(2):e16910
Rationale
The molecular mechanism(s) by which mechanical ventilation disrupts alveolar development, a hallmark of bronchopulmonary dysplasia, is unknown.Objective
To determine the effect of 24 h of mechanical ventilation on lung cell cycle regulators, cell proliferation and alveolar formation in newborn rats.Methods
Seven-day old rats were ventilated with room air for 8, 12 and 24 h using relatively moderate tidal volumes (8.5 mL.kg−1).Measurement and Main Results
Ventilation for 24 h (h) decreased the number of elastin-positive secondary crests and increased the mean linear intercept, indicating arrest of alveolar development. Proliferation (assessed by BrdU incorporation) was halved after 12 h of ventilation and completely arrested after 24 h. Cyclin D1 and E1 mRNA and protein levels were decreased after 8–24 h of ventilation, while that of p27Kip1 was significantly increased. Mechanical ventilation for 24 h also increased levels of p57Kip2, decreased that of p16INK4a, while the levels of p21Waf/Cip1 and p15INK4b were unchanged. Increased p27Kip1 expression coincided with reduced phosphorylation of p27Kip1 at Thr157, Thr187 and Thr198 (p<0.05), thereby promoting its nuclear localization. Similar -but more rapid- changes in cell cycle regulators were noted when 7-day rats were ventilated with high tidal volume (40 mL.kg−1) and when fetal lung epithelial cells were subjected to a continuous (17% elongation) cyclic stretch.Conclusion
This is the first demonstration that prolonged (24 h) of mechanical ventilation causes cell cycle arrest in newborn rat lungs; the arrest occurs in G1 and is caused by increased expression and nuclear localization of Cdk inhibitor proteins (p27Kip1, p57Kip2) from the Kip family. 相似文献110.