全文获取类型
收费全文 | 177篇 |
免费 | 34篇 |
出版年
2021年 | 2篇 |
2020年 | 3篇 |
2018年 | 2篇 |
2017年 | 5篇 |
2015年 | 5篇 |
2014年 | 6篇 |
2013年 | 7篇 |
2012年 | 7篇 |
2011年 | 8篇 |
2010年 | 5篇 |
2009年 | 13篇 |
2008年 | 8篇 |
2007年 | 6篇 |
2006年 | 8篇 |
2005年 | 9篇 |
2004年 | 4篇 |
2003年 | 9篇 |
2002年 | 10篇 |
2001年 | 6篇 |
2000年 | 7篇 |
1999年 | 11篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 5篇 |
1985年 | 3篇 |
1983年 | 4篇 |
1977年 | 1篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 4篇 |
1970年 | 2篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1954年 | 3篇 |
1951年 | 1篇 |
1910年 | 1篇 |
1887年 | 1篇 |
1875年 | 1篇 |
排序方式: 共有211条查询结果,搜索用时 15 毫秒
151.
Tumorigenic poxviruses: construction of the composite physical map of the Shope fibroma virus genome. 总被引:7,自引:4,他引:3 下载免费PDF全文
The sites for the restriction enzymes BamHI, Bg/I, HindIII, PstI, PvuII, and SstI on the linear DNA genome of Shope fibroma virus, a tumorigenic poxvirus of rabbits, have been determined by digestions of the cloned BamHI and HindIII restriction fragments and by hybridization of 32P-labeled cloned fragments to Southern blots of Shope fibroma virus DNA cleaved partially or completely with the various enzymes. The linear genome is shown to be 160 kilobases in length and to possess terminal inverted repeat sequences of between 12.2 and 12.5 kilobases extending inwards from the cross-linked DNA telomeres. The fine map of the Shope fibroma virus terminal inverted repeats has been constructed and shown to be distinctly different from that of members of the orthopoxvirus group, such as vaccinia, by the absence of detectable tandemly repeated sequences near the termini and by the lack of detectable sequence homology with vaccinia termini. 相似文献
152.
S. Lance Macaulay Ashraf S. M. Kelada Joseph Proietto 《Molecular and cellular biochemistry》1994,141(1):27-33
Isoproterenol is a beta adrenergic agonist whose effects have been attributed to the generation of cAMP. Previous studies have shown that it inhibits glucose transport in adipocytes without changing the number of insulin-responsive glucose transporters (GLUT4) on the cell surface. However, we have shown previously that cAMP stimulates translocation of GLUT4 to the cell surface in adipocytes (Keladaet al. J Biol Chem 267, 7021–7025, 1992). We therefore further investigated the mechanisms involved in isoproterenol regulation of glucose transport. Consistent with the effects of dibutyryl cAMP, we found that a low concentration of isoproterenol (10 nM) stimulated glucose transport and the translocation of GLUT4 from the low density microsomal fraction to the plasma membrane. By contrast, a higher concentration of isoproterenol (1 M) did not stimulate transport or GLUT4 translocation and furthermore inhibited dibutyryl cAMP-stimulated GLUT4 translocation. This inhibitory effect was specific for cAMP since isoproterenol had no effect on insulin-stimulated GLUT4 translocation. We conclude that isoproterenol has a biphasic effect on glucose transport, mediated by acute translocation of GLUT4 at low concentrations and by inhibition of intrinsic activity at high concentration, both of which may be explained by effects of cAMP. It has a further cAMP-independent effect at high concentration to inhibit cAMP-mediated translocation of GLUT4.This work forms portions of the PhD thesis requirements. 相似文献
153.
K. E. Bendall V. A. Macaulay J. R. Baker B. C. Sykes 《American journal of human genetics》1996,59(6):1276-1287
As part of an investigation of the fixation mechanisms of mtDNA mutations in humans, we sequenced the first hypervariable segment of the control region in 180 twin pairs and found evidence of site heteroplasmy in 4 pairs. Significant levels of two mitochondrial haplotypes differing by a single point mutation were found in two MZ pairs, and within each pair, both members had similar levels of heteroplasmy. Two DZ pairs were found in which the predominant mitochondrial haplotype differed within the pair. We measured proportions of mitochondrial haplotypes within two twin pairs and their maternal relatives, using primer extension. In both maternal lineages, most family members were heteroplasmic, and the proportions of each genotype varied widely in different individuals. We used the changes in haplotype proportions within mother-offspring pairs to calculate the size range of potential bottlenecks in mitochondrial numbers occurring during development of the offspring. In most individuals, the most likely effective bottleneck sizes ranged from 3 to 20 segregating units, though in two individuals a small bottleneck was very unlikely and there was no upper limit on its possible size. We also used the data from this study, together with unpublished data from other populations, to estimate the frequency of site heteroplasmy in normal human populations. From this, we calculated that the rate of mutation and fixation in the first hypervariable segment of the human mtDNA control region is between 1.2 x 10(-6) and 2.7 x 10(-5) per site per generation. This range is in good agreement with published estimates calculated by other methods. 相似文献
154.
Mark E. Looseley Lucie L. Griffe Bianca Büttner Kathryn M. Wright Jill Middlefell-Williams Hazel Bull Paul D. Shaw Malcolm Macaulay Allan Booth Günther Schweizer Joanne R. Russell Robbie Waugh William T. B. Thomas Anna Avrova 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(12):2513-2528
Key message
Association analyses of resistance to Rhynchosporium commune in a collection of European spring barley germplasm detected 17 significant resistance quantitative trait loci. The most significant association was confirmed as Rrs1.Abstract
Rhynchosporium commune is a fungal pathogen of barley which causes a highly destructive and economically important disease known as rhynchosporium. Genome-wide association mapping was used to investigate the genetic control of host resistance to R. commune in a collection of predominantly European spring barley accessions. Multi-year disease nursery field trials revealed 8 significant resistance quantitative trait loci (QTL), whilst a separate association mapping analysis using historical data from UK national and recommended list trials identified 9 significant associations. The most significant association identified in both current and historical data sources, collocated with the known position of the major resistance gene Rrs1. Seedling assays with R. commune single-spore isolates expressing the corresponding avirulence protein NIP1 confirmed that this locus is Rrs1. These results highlight the significant and continuing contribution of Rrs1 to host resistance in current elite spring barley germplasm. Varietal height was shown to be negatively correlated with disease severity, and a resistance QTL was identified that co-localised with the semi-dwarfing gene sdw1, previously shown to contribute to disease escape. The remaining QTL represent novel resistances that are present within European spring barley accessions. Associated markers to Rrs1 and other resistance loci, identified in this study, represent a set of tools that can be exploited by breeders for the sustainable deployment of varietal resistance in new cultivars.155.
156.
157.
Sequences throughout the basic beta-1,3-glucanase mRNA coding region are targets for homology dependent post-transcriptional gene silencing 总被引:3,自引:0,他引:3
158.
159.
160.
Bart GJ Knols Basilio N Njiru Evan M Mathenge Wolfgang R Mukabana John C Beier Gerry F Killeen 《Malaria journal》2002,1(1):19-13