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41.
Annemarie MM Vlaar Tjerk de Nijs Marinus JPG van Kroonenburgh Werner H Mess Ania Winogrodzka Selma C Tromp Wim EJ Weber 《BMC neurology》2008,8(1):42
Background
Transcranial duplex sonography (TCD) of the substantia nigra has emerged as a promising, non-invasive tool to diagnose idiopathic Parkinson's disease (IPD). However, its diagnostic accuracy in patients with undefined parkinsonism remains to be determined. 相似文献42.
Díaz López E A Morales Ruíz EJ Vargas Ramírez P Hernández Herrera RC Reséndiz Melgar I Brena Hernández JM Loeza Corte 《Phyton》2015,84(2):319-330
The effect of six phosphorus levels (0, 40, 80, 120, 160 and 200 kg/ha) on the duration of cumulative leaf area, biomass and agronomic yield was determined in the maize cultivars: Amarillo Almoloya, Cacahuacintle and Condor in 2010 and 2011. Such cultivars were sown in the Cerrillo Piedras Blancas Mexico. A completely randomized complete block design with factorial arrangement was utilized. High phosphorus levels (120, 160 and 200 kg/ha) positively affected the duration of cumulative leaf area; greatest values were obtained in Cacahuacintle. A greater duration of accumulated leaf area contributes to determine high values of biomass accumulation and grain yield in this cultivar. Leaf area duration appeared to be a useful tool for evaluating different genotypes in a given environment. 相似文献
43.
Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells 总被引:1,自引:1,他引:0
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When L929 cells are exposed to 5 μg/ml dexamethasone, synthesis of a 90,000 M(r) polypeptide is induced within 12 h. Flattening of the cells begins at about this time and progresses to become quite prominent after 48 h of exposure. Two-dimensional PAGE and partial proteolytic fingerprints identify the 90,000 M(r) polypeptide as gelsolin, a Ca(++)-dependent inhibitor of actin polymerization. Thus, this system provides evidence that gelsolin may have a role in regulating cell shape in response to physiological agents such as glucocorticoids. 相似文献
44.
Veera Kainulainen Yurui Tang Thomas Spillmann Susanne Kilpinen Justus Reunanen Per EJ Saris Reetta Satokari 《BMC microbiology》2015,15(1)
Background
For a good probiotic candidate, the abilities to adhere to intestinal epithelium and to fortify barrier function are considered to be crucial for colonization and functionality of the strain. The strain Lactobacillus acidophilus LAB20 was isolated from the jejunum of a healthy dog, where it was found to be the most pre-dominant lactobacilli. In this study, the adhesion ability of LAB20 to intestinal epithelial cell (IECs) lines, IECs isolated from canine intestinal biopsies, and to canine, porcine and human intestinal mucus was investigated. Further, we studied the ability of LAB20 to fortify the epithelial cell monolayer and to reduce LPS-induced interleukin (IL-8) release from enterocytes.Results
We found that LAB20 presented higher adhesion to canine colonic mucus as compared to mucus isolated from porcine colon. LAB20 showed adhesion to HT-29 and Caco-2 cell lines, and importantly also to canine IECs isolated from canine intestinal biopsies. In addition, LAB20 increased the transepithelial electrical resistance (TER) of enterocyte monolayers and thus strengthened the intestinal barrier function. The strain showed also anti-inflammatory capacity in being able to attenuate the LPS-induced IL-8 production of HT-29 cells.Conclusion
In conclusion, canine indigenous strain LAB20 is a potential probiotic candidate for dogs adhering to the host epithelium and showing intestinal barrier fortifying and anti-inflammatory effects.Electronic supplementary material
The online version of this article (doi:10.1186/s12866-014-0337-9) contains supplementary material, which is available to authorized users. 相似文献45.
Background
We consider the potential for infection to spread in a farm population from the primary outbreak farm via livestock movements prior to disease detection. We analyse how this depends on the time of the year infection occurs, the species transmitting, the length of infectious period on the primary outbreak farm, location of the primary outbreak, and whether a livestock market becomes involved. We consider short infectious periods of 1 week, 2 weeks and 4 weeks, characteristic of acute contagious livestock diseases. The analysis is based on farms in Scotland from 1 January 2003 to 31 July 2007.Results
The proportion of primary outbreaks from which an acute contagious disease would spread via movement of livestock is generally low, but exhibits distinct annual cyclicity with peaks in May and August. The distance that livestock are moved varies similarly: at the time of the year when the potential for spread via movements is highest, the geographical spread via movements is largest. The seasonal patterns for cattle differ from those for sheep whilst there is no obvious seasonality for pigs. When spread via movements does occur, there is a high risk of infection reaching a livestock market; infection of markets can amplify disease spread. The proportion of primary outbreaks that would spread infection via livestock movements varies significantly between geographical regions.Conclusions
In this paper we introduce a set-up for analysis of movement data that allows for a generalized assessment of the risk associated with infection spreading from a primary outbreak farm via livestock movements, applying this to Scotland, we assess how this risk depends upon the time of the year, species transmitting, location of the farm and other factors. 相似文献46.
47.
Stefan Richter Rudi Loesel Günter Purschke Andreas Schmidt-Rhaesa Gerhard Scholtz Thomas Stach Lars Vogt Andreas Wanninger Georg Brenneis Carmen Döring Simone Faller Martin Fritsch Peter Grobe Carsten M Heuer Sabrina Kaul Ole S Møller Carsten HG Müller Verena Rieger Birgen H Rothe Martin EJ Stegner Steffen Harzsch 《Frontiers in zoology》2010,7(1):1-49
48.
Douglas E. Coplen Pamela S. Howard John W. Duckett Howard M. Snyder Edward J. Macarak 《In vitro cellular & developmental biology. Animal》1994,30(9):604-608
Summary For the first time we report on the growth, culture, and matrix production characteristics of a cell type isolated from the
lamina propria of the urinary bladder wall. A fibroblastlike cell was identified as distinct from bladder detrusor smooth
muscle cells and urothelium based on morphology, growth characteristics, and immunohistochemical staining. Characterization
of extracellular matrix synthesis by this cell type using35S-methionine metabolic labeling demonstrated that these cells are capable of secreting components of the surrounding connective
tissue, including several fibrillar collagens, a basement membrane collagen, and fibronectin. 相似文献
49.
Smooth muscle trans-membrane sarcoglycan complex in partial bladder outlet obstruction 总被引:2,自引:2,他引:0
Macarak EJ Schulz J Zderic SA Sado Y Ninomiya Y Polyak E Chacko S Howard PS 《Histochemistry and cell biology》2006,126(1):71-82
The urinary bladder experiences both distension and contraction as a part of the normal filling and emptying cycle. To empty properly, tension generated intracellularly in a smooth muscle cell must be smoothly and efficiently transferred across its sarcolemma to the basement membrane, which mediates its binding to both the extracellular matrix and to other cells. As a consequence of urethral obstruction, the bladder cannot generate appropriate force to contract the organ, thereby leading to inefficient emptying and associated sequelae. In this study, an animal model of urethral obstruction was utilized to study the membrane-associated structures that transfer tension across the sarcolemma of bladder smooth muscle cells. Immunohistochemical localization of key components of the smooth muscle tension transfer apparatus (TTA) was performed utilizing specific antibodies against:(1) the α-chains of type IV collagen, a basement membrane component, and (2) β-sarcoglycan, an integral membrane protein that is a participant in the physical linkage between the cytoskeleton and the basement membrane. We demonstrate, in obstructed animals, that there is a pronounced disruption of the TTA with a physical displacement of these two components that can be demonstrated at the level of the light microscope using scanning confocal microscopy. Electron microscopy further demonstrates significant increases in the size of the junctional plaques between smooth muscle cells. 相似文献
50.