全文获取类型
收费全文 | 815篇 |
免费 | 68篇 |
出版年
2022年 | 5篇 |
2021年 | 12篇 |
2020年 | 11篇 |
2019年 | 9篇 |
2018年 | 10篇 |
2017年 | 24篇 |
2016年 | 34篇 |
2015年 | 47篇 |
2014年 | 45篇 |
2013年 | 56篇 |
2012年 | 55篇 |
2011年 | 47篇 |
2010年 | 23篇 |
2009年 | 36篇 |
2008年 | 40篇 |
2007年 | 41篇 |
2006年 | 25篇 |
2005年 | 27篇 |
2004年 | 24篇 |
2003年 | 24篇 |
2002年 | 20篇 |
2001年 | 22篇 |
2000年 | 20篇 |
1999年 | 13篇 |
1998年 | 6篇 |
1996年 | 5篇 |
1994年 | 7篇 |
1992年 | 6篇 |
1991年 | 7篇 |
1990年 | 13篇 |
1989年 | 13篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1985年 | 9篇 |
1984年 | 15篇 |
1983年 | 5篇 |
1981年 | 6篇 |
1979年 | 7篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1975年 | 5篇 |
1973年 | 4篇 |
1972年 | 7篇 |
1971年 | 7篇 |
1970年 | 5篇 |
1967年 | 6篇 |
1966年 | 4篇 |
1888年 | 6篇 |
1887年 | 5篇 |
排序方式: 共有883条查询结果,搜索用时 15 毫秒
71.
72.
Many species of Eucalyptus in Australia provide copious amounts of nectar during their reproductive seasons. The nectar is used by many animal species but especially by birds, insects and some bats, which act as pollinators. One of the major features of eucalypt flowering in southern Australia is the patchy, asynchronous flowering of different species, which appears to drive mass nomadism of nectarivorous birds among regions and among habitats. Here we explore whether flowering asynchrony or climate is primarily responsible for the influxes and effluxes of vast numbers of nectarivorous birds in central Victoria, Australia. By using a structured sampling program, we show that winter flowering by red ironbark Eucalyptus tricarpa is the most likely agent controlling avian-nectarivore dynamics rather than climatic differences among regions. Densities and species richness of nectarivores, and numbers of nectarivory events, are all closely related to measures of flowering intensity. However, nonnectarivores, such as insectivores and granivores, show no relationships with either habitat or region. We discuss how dependence on a patchily distributed but highly rewarding resource such as nectar influences population densities and community structure in birds. 相似文献
73.
Emma del Carmen Macías-Cortés Lidia Llanes-González Leopoldo Aguilar-Faisal Juan Asbun-Bojalil 《PloS one》2015,10(3)
Background
Perimenopausal period refers to the interval when women''s menstrual cycles become irregular and is characterized by an increased risk of depression. Use of homeopathy to treat depression is widespread but there is a lack of clinical trials about its efficacy in depression in peri- and postmenopausal women. The aim of this study was to assess efficacy and safety of individualized homeopathic treatment versus placebo and fluoxetine versus placebo in peri- and postmenopausal women with moderate to severe depression.Methods/Design
A randomized, placebo-controlled, double-blind, double-dummy, superiority, three-arm trial with a 6 week follow-up study was conducted. The study was performed in a public research hospital in Mexico City in the outpatient service of homeopathy. One hundred thirty-three peri- and postmenopausal women diagnosed with major depression according to DSM-IV (moderate to severe intensity) were included. The outcomes were: change in the mean total score among groups on the 17-item Hamilton Rating Scale for Depression, Beck Depression Inventory and Greene Scale, after 6 weeks of treatment, response and remission rates, and safety. Efficacy data were analyzed in the intention-to-treat population (ANOVA with Bonferroni post-hoc test).Results
After a 6-week treatment, homeopathic group was more effective than placebo by 5 points in Hamilton Scale. Response rate was 54.5% and remission rate, 15.9%. There was a significant difference among groups in response rate definition only, but not in remission rate. Fluoxetine-placebo difference was 3.2 points. No differences were observed among groups in the Beck Depression Inventory. Homeopathic group was superior to placebo in Greene Climacteric Scale (8.6 points). Fluoxetine was not different from placebo in Greene Climacteric Scale.Conclusion
Homeopathy and fluoxetine are effective and safe antidepressants for climacteric women. Homeopathy and fluoxetine were significantly different from placebo in response definition only. Homeopathy, but not fluoxetine, improves menopausal symptoms scored by Greene Climacteric Scale.Trial Registration
ClinicalTrials.gov NCT01635218Protocol Publication
http://www.trialsjournal.com/content/14/1/105. 相似文献74.
Bushmeat genetics: setting up a reference framework for the DNA typing of African forest bushmeat
下载免费PDF全文
![点击此处可从《Molecular ecology resources》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Philippe Gaubert Flobert Njiokou Ayodeji Olayemi Paolo Pagani Sylvain Dufour Emmanuel Danquah Mac Elikem K. Nutsuakor Gabriel Ngua Alain‐Didier Missoup Pablo A. Tedesco Rémy Dernat Agostinho Antunes 《Molecular ecology resources》2015,15(3):633-651
The bushmeat trade in tropical Africa represents illegal, unsustainable off‐takes of millions of tons of wild game – mostly mammals – per year. We sequenced four mitochondrial gene fragments (cyt b, COI, 12S, 16S) in >300 bushmeat items representing nine mammalian orders and 59 morphological species from five western and central African countries (Guinea, Ghana, Nigeria, Cameroon and Equatorial Guinea). Our objectives were to assess the efficiency of cross‐species PCR amplification and to evaluate the usefulness of our multilocus approach for reliable bushmeat species identification. We provide a straightforward amplification protocol using a single ‘universal’ primer pair per gene that generally yielded >90% PCR success rates across orders and was robust to different types of meat preprocessing and DNA extraction protocols. For taxonomic identification, we set up a decision pipeline combining similarity‐ and tree‐based approaches with an assessment of taxonomic expertise and coverage of the GENBANK database. Our multilocus approach permitted us to: (i) adjust for existing taxonomic gaps in GENBANK databases, (ii) assign to the species level 67% of the morphological species hypotheses and (iii) successfully identify samples with uncertain taxonomic attribution (preprocessed carcasses and cryptic lineages). High levels of genetic polymorphism across genes and taxa, together with the excellent resolution observed among species‐level clusters (neighbour‐joining trees and Klee diagrams) advocate the usefulness of our markers for bushmeat DNA typing. We formalize our DNA typing decision pipeline through an expert‐curated query database – DNAbushmeat – that shall permit the automated identification of African forest bushmeat items. 相似文献
75.
Gesteira AS Micheli F Carels N Da Silva AC Gramacho KP Schuster I Macêdo JN Pereira GA Cascardo JC 《Annals of botany》2007,100(1):129-140
BACKGROUND AND AIMS: Witches' broom disease is caused by the hemibiotrophic basidiomycete Moniliophthora perniciosa, and is one of the most important diseases of cacao in the western hemisphere. Because very little is known about the global process of such disease development, expressed sequence tags (ESTs) were used to identify genes expressed during the Theobroma cacao-Moniliophthora perniciosa interaction. METHODS: Two cDNA libraries corresponding to the resistant (RT) and susceptible (SP) cacao-M. perniciosa interactions were constructed from total RNA, using the DB SMART Creator cDNA library kit (Clontech). Clones were randomly selected, sequenced from the 5' end and analysed using bioinformatics tools including in silico analysis of the differential gene expression. KEY RESULTS: A total of 6884 ESTs were generated from the RT and SP cDNA libraries. These ESTs were composed of 2585 singlets and 341 contigs for a total of 2926 non-redundant sequences. The redundancy of the libraries was low and their specificity high when compared with the few other cacao libraries already published. Sequence analysis allowed the assignment of a putative functional category for 54 % of sequences, whereas approx. 22 % of sequences corresponded to unknown function and approx. 24 % of sequences did not show any significant similarity with other proteins present in the database. Despite the similar overall distribution of the sequences in functional categories between the two libraries, qualitative differences were observed. Genes involved during the defence response to pathogen infection or in programmed cell death were identified, such as pathogenesis related-proteins, trypsin inhibitor or oxalate oxidase, and some of them showed an in silico differential expression between the resistant and the susceptible interactions. CONCLUSIONS: As far as is known this is the first EST resource from the cacao-M. perniciosa interaction and it is believed that it will provide a significant contribution to the understanding of the molecular mechanisms of the resistance and susceptibility of cacao to M. perniciosa, to develop strategies to control witches' broom, and as a source of polymorphism for molecular marker development and marker-assisted selection. 相似文献
76.
1. Habitat structure long has been identified as a primary factor influencing local assemblage composition. Most evidence has been in the form of correlations of species occurrence and assemblage composition over a range of habitats, with experimental verification of relationships being relatively uncommon because of the difficulties of enacting precise manipulations of habitat structure. 2. Fallen timber (also known as coarse or large woody debris) is one of the few habitat-structural elements in forests and woodlands that can be manipulated with relatively high precision. We report on manipulations of wood-loads on 30 experimental 1-ha plots in floodplain forests of northern Victoria, Australia, over 4 years (one pre- and three post-manipulation). 3. We show that very high wood-loads (80 Mg ha(-1)) and intermediate wood-loads derived from tree crowns (40 Mg ha(-1)) increase species richness (all species and ground-foraging species) and numbers of birds (all species and ground-foraging species) relative to the control plots. 4. Three bird species consistently increased most following manipulations: white-plumed honeyeater Lichenostomus penicillatus (Gould 1837) (fam. Meliphagidae), brown treecreeper Climacteris picumnus (Temm. & Laug. 1824) (fam. Climacteridae) and yellow rosella Platycercus elegans flaveolus (Gould 1837) (fam. Psittacidae). The honeyeater is not considered as a ground or fallen timber dependent species, while the treecreeper and rosella both are regarded as being dependent on ground-layer structure. 5. Fallen timber management needs to be considered in a landscape and temporal context for improving conservation of avian biodiversity. 相似文献
77.
Mitogen-activated protein kinase phosphatase 1/dual specificity phosphatase 1 mediates glucocorticoid inhibition of osteoblast proliferation 总被引:1,自引:0,他引:1
Horsch K de Wet H Schuurmans MM Allie-Reid F Cato AC Cunningham J Burrin JM Hough FS Hulley PA 《Molecular endocrinology (Baltimore, Md.)》2007,21(12):2929-2940
Steroid-induced osteoporosis is a common side effect of long-term treatment with glucocorticoid (GC) drugs. GCs have multiple systemic effects that may influence bone metabolism but also directly affect osteoblasts by decreasing proliferation. This may be beneficial at low concentrations, enhancing differentiation. However, high-dose treatment produces a severe deficit in the proliferative osteoblastic compartment. We provide causal evidence that this effect of GC is mediated by induction of the dual-specificity MAPK phosphatase, MKP-1/DUSP1. Excessive MKP-1 production is both necessary and sufficient to account for the impaired osteoblastic response to mitogens. Overexpression of MKP-1 after either GC treatment or transfection ablates the mitogenic response in osteoblasts. Knockdown of MKP-1 using either immunodepletion of MKP-1 before in vitro dephosphorylation assay or short interference RNA transfection prevents inactivation of ERK by GCs. Neither c-jun N-terminal kinase nor p38 MAPK is activated by the mitogenic cocktail in 20% fetal calf serum, but their activation by a DNA-damaging agent (UV irradiation) was inhibited by either GC treatment or overexpression of MKP-1, indicating regulation of all three MAPKs by MKP-1 in osteoblasts. However, an inhibitor of the MAPK/ERK kinase-ERK pathway inhibited osteoblast proliferation whereas inhibitors of c-jun N-terminal kinase or p38 MAPK had no effect, suggesting that ERK is the MAPK that controls osteoblast proliferation. Regulation of ERK by MKP-1 provides a novel mechanism for control of osteoblast proliferation by GCs. 相似文献
78.
Zonobiomes, zonoecotones and azonal vegetation along the Pacific coast of North America 总被引:1,自引:1,他引:0
In this study of the Pacific coast of North America, from Baja California to Alaska, we evaluated the hypothesis that the
floristic composition of azonal vegetation determines areas and distribution limits similar to those of the corresponding
zonobiomes (ZB), and does so in response to the same macroclimatic changes occurring on the continental scale. To this end,
686 vascular plants of the different habitats found in 279 sites along this coastal strip were recorded. Using an objective
classification system (Average Linkage Clustering) and factorial analysis, floristic data acquired in fieldwork were classified
into groups, which were in turn related to regional macroclimates. Our main finding was that the azonal coastal vegetation
follows a distribution model that is closely linked to the corresponding macroclimate. The four ZB of the northern Pacific
coast show a flora and azonal vegetation characteristic to each zonobiome; the latitudinal limits of the azonal vegetation
practically coinciding with those already established for the zonal vegetation. The Boreal and Temperate ZB show high percentages
of broadly distributed elements. The floristically richest zonobiome in terms of endemic taxa is the Mediterranean zonobiome,
whereas the flora of Baja California is characterized by a high number of taxa related to Neotropical flora, especially to
those showing links with South America. Data on the geographical distribution and habitats of the 247 most significant coastal
species are also provided. 相似文献
79.
80.
Rodrigo Araújo Lima Rodrigues Ludmila Karen dos Santos Silva Fábio Pio Dornas Danilo Bretas de Oliveira Thais Furtado Ferreira Magalh?es Daniel Assis Santos Adriana Oliveira Costa Luiz de Macêdo Farias Paula Prazeres Magalh?es Cláudio Ant?nio Bonjardim Erna Geessien Kroon Bernard La Scola Juliana Reis Cortines J?natas Santos Abrah?o 《Journal of virology》2015,89(23):11812-11819