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121.
Differential effects of nutrient-limited primary production on primary, secondary or tertiary consumers 总被引:2,自引:0,他引:2
Arne M. Malzahn Florian Hantzsche Katherina L. Schoo Maarten Boersma Nicole Aberle 《Oecologia》2010,162(1):35-48
Nutritional imbalances between predator and prey are the rule rather than the exception at the lower end of food webs. We investigated the role of different grazers in the propagation of nutritionally imbalanced primary production by using the same primary producers in a three-trophic-level food chain and a four-trophic-level food chain experimental setup. The three-trophic-level food chain consisted of a classic single-cell primary producer (Rhodomonas salina), a metazoan grazer (the copepod Acartia tonsa) and a top predator (the jellyfish Gonionemus vertens), while we added a protozoan grazer (Oxyrrhis marina) as primary consumer to the food chain to establish the four-trophic-level food chain. This setup allowed us to investigate how nutrient-limitation effects change from one trophic level to another, and to investigate the performance of two components of our experimental food chains in different trophic positions. Stoichiometry and fatty acid profiles of the algae showed significant differences between the nutrient-depleted [no N and no P addition (?P), respectively] and the nutrient-replete (f/2) treatments. The differences in stoichiometry could be traced when O. marina was the first consumer. Copepods feeding on these flagellates were not affected by the nutritional imbalance of their prey in their stoichiometry, their respiration rates nor in their developmental rates. In contrast, when copepods were the primary consumer, those reared on the ?P algae showed significantly higher respiration rates along with significantly lower developmental rates. In neither of our two experimental food chains did the signals from the base of the food chains travel up to jelly fish, our top predator. 相似文献
122.
123.
As seston and chlorophyll concentrations in eutrophic lakesare usually high all year round, it was hypothesized in thepast that food limitation is of major importance for the seasonaldynamics of herbivorous zooplankton in such lakes. Since directmeasurements of food are hampered by the lack of knowledge onthe exact nature of the food in eutrophic conditions, indirectmeasurements are necessary to estimate the degree of food limitationin these circumstances. Hence, we used laboratory- and field-derivedrelationships between the body length and body carbon contentof different species of Daphnia, which were collected from thehighly eutrophic Tjeukemeer, the Netherlands. From a seasonalsurvey of the carbon content of the daphnids. we concluded thatin Tjeukemeer D.galeata, D.galeata x cucullata and D.cucullataare food limited during the largest part of the year. Sincethe condition of the hybrids was relatively high as comparedwith the parental species when the food concentration was high,D.galeata x cucutlaia is expected to be the more successfultaxon during periods of high food availability. 相似文献
124.
Roohparvar R Huser A Zwiers LH De Waard MA 《Applied and environmental microbiology》2007,73(15):5011-5019
Medical drugs known to modulate the activity of human ATP-binding cassette (ABC) transporter proteins (modulators) were tested for the ability to potentiate the activity of the azole fungicide cyproconazole against in vitro growth of Mycosphaerella graminicola and to control disease development due to this pathogen on wheat seedlings. In vitro modulation of cyproconazole activity could be demonstrated in paper disk bioassays. Some of the active modulators (amitriptyline, flavanone, and phenothiazines) increased the accumulation of cyproconazole in M. graminicola, suggesting that they reversed cyproconazole efflux. However, synergism between cyproconazole and modulators against M. graminicola on wheat seedlings could not be shown. Despite their low in vitro toxicity to M. graminicola, some modulators (amitriptyline, loperamide, and promazine) did show significant intrinsic disease control activity in preventive and curative foliar spray tests with wheat seedlings. The results suggest that these compounds have indirect disease control activity based on modulation of fungal ABC transporters essential for virulence and constitute a new class of disease control agents. 相似文献
125.
Wielockx B Staelens J Puimège L Vanlaere I Van Roy M van Lint P Van Roy F Libert C 《Journal of immunology (Baltimore, Md. : 1950)》2007,178(8):5069-5075
In our search for genes that inhibit the inflammatory effects of TNF without diminishing its antitumor capacities we found that, compared with C57BL/6 mice, DBA/2 mice exhibit a dominant resistance to TNF-induced lethality. Tumor-bearing (C57BL/6 x DBA/2)(BXD)F(1) mice completely survived an otherwise lethal TNF/IFN-gamma-antitumor therapy with complete regression of the tumor. This was not the case for C57BL/6 mice. Genetic linkage analysis revealed that TNF resistance is linked to a major locus on distal chromosome 6 and a minor locus on chromosome 17. Compared with littermate controls, chromosome substitution mice carrying a DBA/2 chromosome 6 in a C57BL/6 background were significantly protected against TNF and TNF/IFN-gamma, albeit less so than DBA/2 mice. Definition of a critical region of 13 Mb on chromosome 6 was the highest mapping resolution obtained. Further analysis of candidate genes may provide a powerful tool to control TNF-induced pathologies in humans. 相似文献
126.
Glutamate transporters are trimeric membrane proteins in which each protomer contains a separate translocation path. To determine whether structural rearrangements take place at the subunit interfaces during transport, intersubunit disulfide bridges were introduced in the bacterial transporter GltT. None of the intersubunit cross-links, which had been designed across the entire interface, affected the glutamate transport activity, indicating that the subunit interfaces are rigid during turnover. 相似文献
127.
von Stetten D Seibeck S Michael N Scheerer P Mroginski MA Murgida DH Krauss N Heyn MP Hildebrandt P Borucki B Lamparter T 《The Journal of biological chemistry》2007,282(3):2116-2123
The mutants H250A and D197A of Agp1 phytochrome from Agrobacterium tumefaciens were prepared and investigated by different spectroscopic and biochemical methods. Asp-197 and His-250 are highly conserved amino acids and are part of the hydrogen-bonding network that involves the chromophore. Both substitutions cause a destabilization of the protonated chromophore in the Pr state as revealed by resonance Raman and UV-visible absorption spectroscopy. Titration experiments demonstrate a lowering of the pK(a) from 11.1 (wild type) to 8.8 in H250A and 7.2 in D197A. Photoconversion of the mutants does not lead to the Pfr state. H250A is arrested in a meta-Rc-like state in which the chromophore is deprotonated. For H250A and the wild-type protein, deprotonation of the chromophore in meta-Rc is coupled to the release of a proton to the external medium, whereas the subsequent proton re-uptake, linked to the formation of the Pfr state in the wild-type protein, is not observed for H250A. No transient proton exchange with the external medium occurs in D197A, suggesting that Asp-197 may be the proton release group. Both mutants do not undergo the photo-induced protein structural changes that in the wild-type protein are detectable by size exclusion chromatography. These conformational changes are, therefore, attributed to the meta-Rc --> Pfr transition and most likely coupled to the transient proton re-uptake. The present results demonstrate that Asp-197 and His-250 are essential for stabilizing the protonated chromophore structure in the parent Pr state, which is required for the primary photochemical process, and for the complete photo-induced conversion to the Pfr state. 相似文献
128.
129.
Localization of metabotropic glutamate receptors in the outer plexiform layer of the goldfish retina
We studied the localization of metabotropic glutamate receptors (mGluRs) in the goldfish outer plexiform layer by light-and
electron-microscopical immunohistochemistry. The mGluR1α antibody labeled putative ON-type bipolar cell dendrites and horizontal
cell processes in both rod spherules and cone triads. Immunolabeling for mGluR2/3 was absent in the rod synaptic complex but
was found at horizontal cell dendrites directly opposing the cone synaptic ribbon. The mGluR5 antibody labeled Müller cell
processes wrapping rod terminals and horizontal cell somata. The mGluR7 antibody labeled mainly horizontal cell dendrites
invaginating rods and cones and some putative bipolar cell dendrites in the cone synaptic complex. The finding of abundant
expression of various mGluRs in bipolar and horizontal cell dendrites suggests multiple sites of glutamatergic modulation
in the outer retina.
Financial support for this work was provided by Conselho Nacional de Pesquisa (CNPq), Brazil (grant 200915/98-3 to C.J.) 相似文献
130.
External phosphorus load to a wetland with two shallow lakes in the Botshol Nature Reserve, The Netherlands, was reduced,
resulting in a rapid reduction of phytoplankton biomass and turbidity, and after 4 years, explosive growth of Characeae. The
clear water state was unstable, however, and the ecosystem then alternated between clear, high-vegetation and turbid, low-vegetation
states. A model of water quality processes was used in conjunction with a 14-year nutrient budget for Botshol to determine
if fluctuations in precipitation and nutrient load caused the ecosystem instability. The results indicate that, during wet
winters when groundwater level rose above surface water level, phosphorus from runoff was stored in the lake bottom and banks.
Stored phosphorus was released the following spring and summer under anaerobic sediment conditions, resulting in increased
phytoplankton density and light attenuation in the water column. During years with high net precipitation, flow from land
to surface water also transported humic acids, further increasing light attenuation. In years with dry winters, the phosphorus
and humic acid loads to surface water were reduced, and growth of submerged macrophytes was enhanced by clear water. Thus,
the temporal pattern of precipitation and flow from land to water gave a coherent, quantitative explanation of the observed
dynamics in phosphorus, phytoplankton, turbidity, and Characeae. Global warming has caused winters in The Netherlands to become
warmer and wetter during the last 50 years, increasing flow from land to water of humic acids and phosphorus and, ultimately,
enhancing instability of Characeae populations. In the first half of the 20th century interannual variation in precipitation
was not sufficient to cause large changes in internal P flux in Botshol, and submerged macrophyte populations were stable. 相似文献