全文获取类型
收费全文 | 1451篇 |
免费 | 151篇 |
专业分类
1602篇 |
出版年
2023年 | 12篇 |
2022年 | 20篇 |
2021年 | 32篇 |
2020年 | 22篇 |
2019年 | 23篇 |
2018年 | 25篇 |
2017年 | 32篇 |
2016年 | 57篇 |
2015年 | 89篇 |
2014年 | 107篇 |
2013年 | 103篇 |
2012年 | 136篇 |
2011年 | 108篇 |
2010年 | 76篇 |
2009年 | 55篇 |
2008年 | 96篇 |
2007年 | 91篇 |
2006年 | 77篇 |
2005年 | 61篇 |
2004年 | 58篇 |
2003年 | 44篇 |
2002年 | 58篇 |
2001年 | 16篇 |
2000年 | 7篇 |
1999年 | 14篇 |
1998年 | 12篇 |
1997年 | 17篇 |
1996年 | 13篇 |
1995年 | 17篇 |
1994年 | 17篇 |
1993年 | 13篇 |
1992年 | 15篇 |
1991年 | 10篇 |
1990年 | 6篇 |
1989年 | 7篇 |
1988年 | 12篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1976年 | 3篇 |
1974年 | 2篇 |
1973年 | 3篇 |
1972年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有1602条查询结果,搜索用时 15 毫秒
911.
The BANYULS gene encodes a DFR-like protein and is a marker of early seed coat development 总被引:10,自引:0,他引:10
Martine Devic Jocelyne Guilleminot Isabelle Debeaujon Nicole Bechtold Emmanuelle Bensaude Maarten Koornneef Georges Pelletier Michel Delseny 《The Plant journal : for cell and molecular biology》1999,19(4):387-398
Mutations in the BANYULS (BAN) gene lead to precocious accumulation of anthocyanins in immature seed coat in Arabidopsis. The ban -1 allele has been isolated from a collection of T-DNA transformants and found to be tagged by the integrative molecule. The sequencing of wild-type and two independent mutant alleles confirmed the identity of the gene. Analysis of the full-length cDNA sequence revealed an open reading frame encoding a 342 amino acid protein which shared strong similarities with DFR and other enzymes of the phenylpropanoid biosynthesis pathway. BAN expression was restricted to the endothelium of immature seeds at the pre-globular to early globular stages of development as predicted from the maternal inheritance of the phenotype, and therefore represents a marker for early differentiation and development of the seed coat. BAN is probably involved in a metabolic channelling between the production of anthocyanins and pro-anthocyanidins in the seed coat. 相似文献
912.
913.
Maarten B. Eppinga Max Rietkerk Martin J. Wassen Peter C. De Ruiter 《Plant Ecology》2009,200(1):53-68
Paleoecological studies indicate that peatland ecosystems may exhibit bistability. This would mean that these systems are
resilient to gradual changes in climate, until environmental thresholds are passed. Then, ecosystem stability is lost and
rapid shifts in surface and vegetation structure at landscape scale occur. Another remarkable feature is the commonly observed
self-organized spatial vegetation patterning, such as string-flark and maze patterns. Bistability and spatial self-organization
may be mechanistically linked, the crucial mechanism being scale-dependent (locally positive and longer-range negative) feedback
between vegetation and the peatland environment. Focusing on bogs, a previous model study shows that nutrient accumulation
by vascular plants can induce such scale-dependent feedback driving pattern formation. However, stability of bog microforms
such as hummocks and hollows has been attributed to different local interactions between Sphagnum, vascular plants, and the bog environment. Here we analyze both local and longer-range interactions in bogs to investigate
the possible contribution of these different interactions to vegetation patterning and stability. This is done by a literature
review, and subsequently these findings are incorporated in the original model. When Sphagnum and encompassing local interactions are included in this model, the boundaries between vegetation types become sharper and
also the parameter region of bistability drastically increases. These results imply that vegetation patterning and stability
of bogs could be synergistically governed by local and longer-range interactions. Studying the relative effect of these interactions
is therefore suggested to be an important component of future predictions on the response of peatland ecosystems to climatic
changes. 相似文献
914.
Bacillus species are valuable producers of industrial enzymes and biopharmaceuticals, because they can secrete large quantities of high-quality proteins directly into the growth medium. This requires the concerted action of quality control factors, such as folding catalysts and 'cleaning proteases'. The expression of two important cleaning proteases, HtrA and HtrB, of Bacillus subtilis is controlled by the CssRS two-component regulatory system. The induced CssRS-dependent expression of htrA and htrB has been defined as a protein secretion stress response, because it can be triggered by high-level production of secreted alpha-amylases. It was not known whether translocation of these alpha-amylases across the membrane is required to trigger a secretion stress response or whether other secretory proteins can also activate this response. These studies show for the first time that the CssRS-dependent response is a general secretion stress response which can be triggered by both homologous and heterologous secretory proteins. As demonstrated by high-level production of a nontranslocated variant of the alpha-amylase, AmyQ, membrane translocation of secretory proteins is required to elicit this general protein secretion stress response. Studies with two other secretory reporter proteins, lipase A of B. subtilis and human interleukin-3, show that the intensity of the protein secretion stress response only partly reflects the production levels of the respective proteins. Importantly, degradation of human interleukin-3 by extracellular proteases has a major impact on the production level, but only a minor effect on the intensity of the secretion stress response. 相似文献
915.
Dejan M. Petrović Kees Leenhouts Maarten L. van Roosmalen Jaap Broos 《Amino acids》2013,44(5):1329-1336
Biosynthetic incorporation of tryptophan (Trp) analogues in recombinant proteins using an E. coli Trp auxotroph expression host is limited to analogues modified with a small substituent like a fluoro atom or a hydroxyl or amine group. We report here the efficient incorporation (>89 %) of chloro- and bromo atoms containing Trp analogues in alloproteins at high expression levels using a Lactococcus lactis Trp auxotroph strain. This result was only obtained after coexpression of the enzyme tryptophanyl-tRNA synthetase (TrpRS) of L. lactis, an enzyme believed to show a more relaxed substrate specificity than TrpRS from E. coli. Chloro- and bromo-Trps are attractive intrinsic phosphorescence probes as these Trp analogues are much less sensitive for quenchers in the medium, like oxygen, than Trp. Coexpression of TrpRS was also essential for the biosynthetic incorporation (94 %) of the Trp analogue 5,6 difluoroTrp. This makes our expression system ideally suited to generate a set of methyl- and fluoro-substituted Trp analogue-containing alloproteins in high yield for investigating the involvement of the Trp residue in cation-pi or pi–pi interactions. Taken together, the presented Trp auxotroph expression system features the most relaxed specificity for Trp analogue structures reported to date and gives a high alloprotein yield. 相似文献
916.
Monteferrante CG Baglieri J Robinson C van Dijl JM 《Applied and environmental microbiology》2012,78(14):4999-5001
Two independent twin-arginine translocases (Tat) for protein secretion were previously identified in the Gram-positive bacterium Bacillus subtilis. These consist of the TatAd-TatCd and TatAy-TatCy subunits. The function of a third TatA subunit named TatAc was unknown. Here, we show that TatAc can form active protein translocases with TatCd and TatCy. 相似文献
917.
Ülle Sillasoo Anneli Poska Heikki Seppä Maarten Blaauw Frank M. Chambers 《Vegetation History and Archaeobotany》2009,18(4):315-327
Connections between environmental and cultural changes are analysed in Estonia during the past c. 4,500 years. Records of cereal-type pollen as (agri)cultural indices are compared with high-resolution palaeohydrological and annual mean temperature reconstructions from a selection of Estonian bogs and lakes (and Lake Igelsjön in Sweden). A broad-scale comparison shows increases in the percentage of cereal-type pollen during a decreasing trend in annual mean temperatures over the past c. 4,300 years, suggesting a certain independence of agrarian activities from environmental conditions at the regional level. The first cereal-type pollen in the region is found from a period with a warm and dry climate. A slow increase in pollen of cultivated land is seen around the beginning of the late Bronze Age, a slight increase at the end of the Roman Iron Age and a significant increase at the beginning of the Middle Ages. In a few cases increases in agricultural pollen percentages occur in the periods of warming. Stagnation and regression occurs in the periods of cooling, but regression at individual sites may also be related to warmer climate episodes. The cooling at c. 400–300 cal b.p., during the ‘Little Ice Age’ coincides with declines in cereal-type and herb pollen curves. These may not, however, be directly related to the climate change, because they coincide with war activities in the region. 相似文献
918.
Felix Liechti Janine Aschwanden Jan Blew Mathieu Boos Robin Brabant Adriaan M. Dokter Vladislav Kosarev Maryna Lukach Mercedes Maruri Maarten Reyniers Inbal Schekler Heiko Schmaljohann Baptiste Schmid Nadja Weisshaupt Nir Sapir 《Ecography》2019,42(5):887-898
Large parts of the continents are continuously scanned by terrestrial weather radars to monitor precipitation and wind conditions. These systems also monitor the mass movements of bird, bat, and insect migration, but it is still unknown how many of these systems perform with regard to detection and quantification of migration intensities of the different groups. In this study that was undertaken within five regions across Europe and the Middle East we examined to what extent bird migration intensities derived from different weather radars are comparable between each other and relate to intensities measured by local small‐scaled radars, some of them specifically developed to monitor birds. Good correspondence was found for the relative day‐to‐day pattern in migration intensities among most radar systems that were compared. Absolute intensities varied between different systems and regions. The findings of this study can be used to infer about absolute bird migration intensities measured by different radar systems and consequently help resolving methodological issues regarding the estimation of migrant numbers in the Western‐Palearctic region. It further depicts a scientific basis for the future monitoring of migratory bird populations across a large spatio‐temporal scale, predicting their movements and studying its consequences on ecological systems and human lives. 相似文献
919.
Noémie De Zutter Maarten Ameye Jane Debode Caroline De Tender Sarah Ommeslag Jan Verwaeren Pieter Vermeir Kris Audenaert Leen De Gelder 《Microbial biotechnology》2021,14(4):1594-1612
Phosphorus (P) is despite its omnipresence in soils often unavailable for plants. Rhizobacteria able to solubilize P are therefore crucial to avoid P deficiency. Selection for phosphate-solubilizing bacteria (PSB) is frequently done in vitro; however, rhizosphere competence is herein overlooked. Therefore, we developed an in planta enrichment concept enabling simultaneous microbial selection for P-solubilization and rhizosphere competence. We used an ecologically relevant combination of iron- and aluminium phosphate to select for PSB in maize (Zea mays L.). In each consecutive enrichment, plant roots were inoculated with rhizobacterial suspensions from plants that had grown in substrate with insoluble P. To assess the plants’ P statuses, non-destructive multispectral imaging was used for quantifying anthocyanins, a proxy for maize’s P status. After the third consecutive enrichment, plants supplied with insoluble P and inoculated with rhizobacterial suspensions showed a P status similar to plants supplied with soluble P. A parallel metabarcoding approach uncovered that the improved P status in the third enrichment coincided with a shift in the rhizobiome towards bacteria with plant growth-promoting and P-solubilizing capacities. Finally, further consecutive enrichment led to a functional relapse hallmarked by plants with a low P status and a second shift in the rhizobiome at the level of Azospirillaceae and Rhizobiaceae. 相似文献
920.
Hanne Hoskens Dongjing Liu Sahin Naqvi Myoung Keun Lee Ryan J. Eller Karlijne Indencleef Julie D. White Jiarui Li Maarten H. D. Larmuseau Greet Hens Joanna Wysocka Susan Walsh Stephen Richmond Mark D. Shriver John R. Shaffer Hilde Peeters Seth M. Weinberg Peter Claes 《PLoS genetics》2021,17(5)
The analysis of contemporary genomic data typically operates on one-dimensional phenotypic measurements (e.g. standing height). Here we report on a data-driven, family-informed strategy to facial phenotyping that searches for biologically relevant traits and reduces multivariate 3D facial shape variability into amendable univariate measurements, while preserving its structurally complex nature. We performed a biometric identification of siblings in a sample of 424 children, defining 1,048 sib-shared facial traits. Subsequent quantification and analyses in an independent European cohort (n = 8,246) demonstrated significant heritability for a subset of traits (0.17–0.53) and highlighted 218 genome-wide significant loci (38 also study-wide) associated with facial variation shared by siblings. These loci showed preferential enrichment for active chromatin marks in cranial neural crest cells and embryonic craniofacial tissues and several regions harbor putative craniofacial genes, thereby enhancing our knowledge on the genetic architecture of normal-range facial variation. 相似文献