Animal Life Without Oxygen: Basic Biochemical Mechanisms

A variety of animals are now known to be facultative anaerobes,capable of utilizing molecular oxygen when it is present andcapable of sustained anaerobiosis when it is absent. Duringanoxia these organisms rely upon the simultaneous catabolismof carbohydrate and amino acids. In probing the mechanisms utilized,this essay accounts for (1) the maintenance of redox balanceduring anoxia, (2) the sources of energy in the form of ATP,and (3) the formation of a multiplicity of anaerobic end products.     

Effects of Hydrostatic Pressure on Catalysis by Epaxial Muscle Phosphofructokinase from an Abyssal Fish

SYNOPSIS: Phosphofructokinase (PFK) extracted from muscle ofabyssal Coryphaenoides fishes common in the deep waters aroundthe Galápagos Archipelago is extremely unstable upondecompression and extraction and can be recovered only in lowactivities. Preliminary studies indicate that the pressure responsesof the enzyme are complex: At low pressures, the enzyme is activated;at moderate pressures, activity passes through a pressure optimum;at high pressures, maximum catalytic activity is decelerated.At low pressures, the volume change of activation is about –11cm³/mole at 3°C and increases to about –46 cm³/moleat 28°C. The homologous enzyme from a surface species (Oligoplitesmundus) appears to be more pressure sensitive at low temperatures.     

Structural biomass partitioning in regrowth and undisturbed mesquite (Prosopis glandulosa): implications for bioenergy uses

Honey mesquite ( Prosopis glandulosa Torr.) which grows on grasslands and rangelands in southwestern USA may have potential as a bioenergy feedstock because of existing standing biomass and regrowth potential. However, regrowth mesquite physiognomy is highly different from undisturbed mesquite physiognomy and little is known regarding growth rates and structural biomass allocation in regrowth mesquite. We compared canopy architecture, aboveground biomass and relative allocation of biomass components in regrowth (RG) trees of different known ages with undisturbed (UD) trees of similar canopy height to each RG age class. RG trees in most age classes (2–12 years old) had greater canopy area, leaf area, basal stem number, twig (<0.5 cm diameter) mass and small stem (0.5–3 cm diameter) mass than UD trees of the same height. Large stem (>3 cm diameter) mass was similar between RG and UD trees in all height classes. Ages of UD trees were determined after harvest and further comparisons were made between age, canopy structure and biomass in RG and UD trees. Relationships between age and total mass, age and height, and age and canopy area indicated a faster growth rate in RG than in UD trees. Large stem mass as a percentage of total tree mass accumulated more rapidly with age in RG than UD trees. Leaf area index and leaf : twig mass ratio were maintained near 1 in all RG and UD trees. Regrowth potential may be one of the most important features of mesquite in consideration as a bioenergy feedstock.     

Muscari turcicum (Liliaceae/Hyacinthaceae), a new species from south Anatolia, Turkey

Muscari turcicum Uysal, Ertugrul & Dural (Liliaceae/Hyacinthaceae) is described and illustrated from south Anatolia, Turkey. This species grows on alpine steppe in the Middle Taurus (C4 Konya Province). Muscari turcicum , an endemic confined to the Middle Taurus, is closely related to M. discolor Boiss. & Hausskn. and M. anatolicum Cowley & Özhatay. Diagnostic morphological characters are discussed and compared with those of closely related taxa.  © 2007 The Linnean Society of London, Botanical Journal of the Linnean Society , 2007, 154 , 233–236.     

Translation of a standardized manufacturing protocol for mesenchymal stromal cells: A systematic comparison of validation and manufacturing data

BackgroundMany data are available on expansion protocols for mesenchymal stromal cells (MSCs) for both experimental settings and manufacturing for clinical trials. However, there is a lack of information on translation of established protocols for Good Manufacturing Practice (GMP) from validation to manufacturing for clinical application. We present the validation and translation of a standardized pre-clinical protocol for isolation and expansion of MSCs for a clinical trial for reconstitution of alveolar bone.MethodsKey parameters of 22 large-scale expansions of MSCs from bone marrow (BM) for validation were compared with 11 expansions manufactured for the clinical trial “Jaw bone reconstruction using a combination of autologous mesenchymal stromal cells and biomaterial prior to dental implant placement (MAXILLO1)” aimed at reconstruction of alveolar bone.ResultsDespite variations of the starting material, the robust protocol led to stable performance characteristics of expanded MSCs. Manufacturing of the autologous advanced therapy medicinal product MAXILLO-1-MSC was possible, requiring 21 days for each product. Transport of BM aspirates and MSCs within 24 h was guaranteed. MSCs fulfilled quality criteria requested by the national competent authority. In one case, the delivered MSCs developed a mosaic in chromosomal finding, showing no abnormality in differentiation capacity, growth behavior or surface marker expression during long-term culture. The proportion of cells with the mosaic decreased in long-term culture and cells stopped growth after 38.4 population doublings.ConclusionsClinical use of freshly prepared MSCs, manufactured according to a standardized and validated protocol, is feasible for bone regeneration, even if there was a long local distance between manufacturing center and clinical site. Several parameters, such as colony forming units fibroblasts (CFU-F), percentage of CD34+ cells, cell count of mononuclear cells (MNCs) and white blood cells (WBCs), of the BM may serve as a predictive tool for the yield of MSCs and may help to avoid unnecessary costs for MSC manufacturing due to insufficient cell expansion rates.     

Length-weight relationship and condition factor of common minnow, Esomus danricus (Ham.) from different freshwater environments.

Investigations were made on the length-weight relationship and condition factor of Esomu danricus (Ham) from ponds and flowing water channels. These relationships could be represented by the regression equations, log W = -2.6252 + 3.2451 log L (for channel fishes) and log W = -1.5390 + 2.6318 log L (for pond fishes). The correlations were significantly high (P < 0.001). The mean values of condition factors were 0.529 and 0.713 for pond and channel fishes, respectively. The additive action of various factors in stagnant ponds, such as oxygen deficiency, stratification of indispensahle nutrients at the bottom, accumulation of body wastes of fishes, and unoxidized chemical compounds, competition for many necessities of life as space, food, shelter, seemed to adversely affect the growth rate and condition factor of pond dwelling fishes. Contrary to this the circulation of water induced by currents in the channels appeared to cause mixing of water at all depths to even out differences in dissolved oxygen concentration, temperature, nutrients, besides minimizing the effect of repressive factor. Such conditions invariably offered more opportunities to fishes to conserve almost all the available space and other resources in the habitat. This ensured rapid growth as evidenced by higher condition factor of the flowing-water fishes as compared to their counterparts occurring in ponds.     

Selective feeding behaviour of the common carp Esomus danricus (Ham.) in its natural habitat

Food selectivity by the common freshwater carp, Esomus danricus (Ham.) was studied through Ivlev's equation of electivity. The selective feeding seemed to be influenced by many factors, including the accessibility, abundance and taste, of the food items, the mechanical and physiological adaptations on the part of the fish to capture and digest those food organisms, as also the inherent and instinctive property of the fish to prefer certain types of food over the others. The absence of strong specific selectivity by the fish for a variety of planktonic food organisms was found to be a phenomenon compensatory to stenophagism, in order to make use of whichever food items remained in the habitat at the time when the preferred genera of plankton were not available, due to the process of succession.     

Observations on the effect of photoperiod on the control of polymorphism in Psylla pyricola

ABSTRACT. Psylla pyricola Forster (Homoptera) occurs as two adult morphs, a summer form (f. typica) and a winter form (f. simulans). The latter is larger, darker and overwinters in ovarian diapause. Eggs laid by either morph under LD 12:12 (short days) or under LD 18:6 or 16:8 (long days) gave f. typica when reared under long days. When reared under short days, almost all were f. simulans. No interforms were recorded. When the photoperiod was switched during the nymphal stage (whether from long to short days, diapause induction; or from short to long days, diapause termination), nymphs were progressively less sensitive with increasing age, with no morph changes when the switch occurred in the fifth instar. Nymphs derived from f. simulans reared under long days tended to be more sensitive to a change to short days in the early instars than when the nymphs were first reared under short days and then switched to long days. Interforms were frequent when the switch occurred during the nymphal stage, particularly in the third instar. Wing lengths varied within morphs depending on the timing of the switch. The largest f. simulans were derived from f. typica when maintained under short days, and the smallest f. typica from either morph when maintained under long days, as would be expected under field conditions. No f. simulans were found under field conditions in the first f. typica generation, but a small percentage were present in the second generation. It is concluded that, even within a given generation, not all P. pyricola are equally sensitive to photoperiod, but that photoperiod is one of the major factors controlling the change to f. simulans in the autumn. Whilst under laboratory conditions photoperiod can also affect the switch to f. typica in the spring, in the field it may be less important than low temperatures.     

Heterogonic development of the head, thorax and abdomen in Gryllus domesticus (Orthoptera:Gryllidae)

The heterogonic development of the three basic divisions of the body (head, thorax and abdomen) of Gryllus domesticus , was studied. The relationships in the linear dimensions of each of these parts with the standard parameter (absolute body length) were established through regression equations and the cephalosomatic, thoraco-somatic and abdomeno-somatic ratios. Of the body parts investigated the head was found to be the most heterogonic component followed by the abdomen and the thorax. The heterogonic development in the insect body seemed to be the result of varying intensities of the growth potential of different anatomical locations.