全文获取类型
收费全文 | 1088篇 |
免费 | 72篇 |
国内免费 | 2篇 |
专业分类
1162篇 |
出版年
2024年 | 3篇 |
2023年 | 6篇 |
2022年 | 15篇 |
2021年 | 27篇 |
2020年 | 9篇 |
2019年 | 16篇 |
2018年 | 34篇 |
2017年 | 22篇 |
2016年 | 33篇 |
2015年 | 45篇 |
2014年 | 52篇 |
2013年 | 84篇 |
2012年 | 89篇 |
2011年 | 101篇 |
2010年 | 46篇 |
2009年 | 48篇 |
2008年 | 71篇 |
2007年 | 53篇 |
2006年 | 57篇 |
2005年 | 64篇 |
2004年 | 59篇 |
2003年 | 49篇 |
2002年 | 31篇 |
2001年 | 16篇 |
2000年 | 20篇 |
1999年 | 14篇 |
1998年 | 9篇 |
1997年 | 9篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1994年 | 2篇 |
1993年 | 7篇 |
1992年 | 5篇 |
1991年 | 13篇 |
1990年 | 4篇 |
1989年 | 10篇 |
1988年 | 1篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 5篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1974年 | 2篇 |
1972年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有1162条查询结果,搜索用时 0 毫秒
81.
82.
83.
Monocrotophos inhibited monoamine oxidase (MAO) activity both in vitro and in vivo in liver, kidney and brain areas of albino rats. In vitro effect was more pronounced than the in vivo effect. During in vivo daily treatment with sublethal doses of Monocrotophos, the MAO activity was significantly inhibited after 1h to 7 days of treatments. Later recovery of inhibition was noticed which might be attributed to the enhanced absorption of Monocrotophos to protein and fat bodies or enhanced metabolic dispositional mechanisms. Brain areas exhibit varied responses to Monocrotophos toxicity. 相似文献
84.
Vince JE Wong WW Khan N Feltham R Chau D Ahmed AU Benetatos CA Chunduru SK Condon SM McKinlay M Brink R Leverkus M Tergaonkar V Schneider P Callus BA Koentgen F Vaux DL Silke J 《Cell》2007,131(4):682-693
XIAP prevents apoptosis by binding to and inhibiting caspases, and this inhibition can be relieved by IAP antagonists, such as Smac/DIABLO. IAP antagonist compounds (IACs) have therefore been designed to inhibit XIAP to kill tumor cells. Because XIAP inhibits postmitochondrial caspases, caspase 8 inhibitors should not block killing by IACs. Instead, we show that apoptosis caused by an IAC is blocked by the caspase 8 inhibitor crmA and that IAP antagonists activate NF-kappaB signaling via inhibtion of cIAP1. In sensitive tumor lines, IAP antagonist induced NF-kappaB-stimulated production of TNFalpha that killed cells in an autocrine fashion. Inhibition of NF-kappaB reduced TNFalpha production, and blocking NF-kappaB activation or TNFalpha allowed tumor cells to survive IAC-induced apoptosis. Cells treated with an IAC, or those in which cIAP1 was deleted, became sensitive to apoptosis induced by exogenous TNFalpha, suggesting novel uses of these compounds in treating cancer. 相似文献
85.
Harsh Chauhan Srinivas A. Desai Paramjit Khurana 《Plant Cell, Tissue and Organ Culture》2007,91(3):191-199
An efficient genotype independent, in vitro regeneration system was developed for nine popular Indian wheat cultivars, three
each of Triticum aestivum L. viz., CPAN1676, HD2329 and PBW343, Triticum durum Desf. viz., PDW215, PDW233 and WH896, and Triticum dicoccum Schrank. Schubl. viz., DDK1001, DDK1025 and DDK1029, by manipulating the concentration and time of exposure to the growth
regulator, thidiazuron (TDZ). A total of 18 (for immature inflorescence and embryo explant) and six (for mature embryo explant)
different combinations of growth regulators were tried for callusing and regeneration, respectively. Media combination with
low concentration of TDZ (2.2 μM) in combination to auxin and/or cytokinin (depending upon culture stage), was found to be
effective for immature and mature explants. Compact, nodular and highly embryogenic calli were obtained by using immature
embryo, immature inflorescence and mature embryo explants, and regeneration frequency up to 25 shoots/explant with an overall
80% regeneration was achieved. Comparable regeneration frequency was achieved for mature embryo explants. No separate hormone
combination for rooting was required and plantlets ready to transfer to soil could be obtained in a short period of 8–10 weeks.
This protocol can be used for raising transgenic plants for functional genomics analysis of agronomically important traits
in the three species of wheat. 相似文献
86.
Mitogen-activated protein kinase kinase-4 promotes cell survival by decreasing PTEN expression through an NF kappa B-dependent pathway 总被引:2,自引:0,他引:2
Xia D Srinivas H Ahn YH Sethi G Sheng X Yung WK Xia Q Chiao PJ Kim H Brown PH Wistuba II Aggarwal BB Kurie JM 《The Journal of biological chemistry》2007,282(6):3507-3519
87.
Balaji KN Goyal G Narayana Y Srinivas M Chaturvedi R Mohammad S 《Microbes and infection / Institut Pasteur》2007,9(3):271-281
Ectopic expression of the Mycobacterium tuberculosis PE-family gene Rv1818c, triggers apoptosis in the mammalian Jurkat T cells, which is blocked by anti-apoptotic protein Bcl-2. Although complete overlap is not observed, a considerable proportion of cellular pools of ectopically expressed Rv1818c localizes to mitochondria. However, recombinant Rv1818c does not trigger release of cytochrome c from isolated mitochondria even though Rv1818c protein induced apoptosis of Jurkat T cells. Apoptosis induced by Rv1818c is blocked by the broad-spectrum caspase inhibitory peptide zVAD-FMK. Unexpectedly, Rv1818c-induced apoptosis is not blocked in a Jurkat sub-clone deficient for caspase-8 (JI 9.2) or in cells where caspase-9 function is inhibited or expression of caspase-9 reduced by siRNA, arguing against a central role for these caspases in Rv1818c-induced apoptotic signaling. Depleting cellular pools of the mitochondrial protein Smac/DIABLO substantially reduces apoptosis consistent with mitochondrial involvement in this death pathway. We present evidence that Rv1818c-induced apoptosis is blocked by the co-transfection of an endogenous inhibitor of caspase activation, XIAP in T cells. Additionally, Rv1818c is released into extracellular environment via exosomes secreted by M. tuberculosis infected BM-DC's and macrophages. Furthermore, the extracellular Rv1818c protein can be detected in T cells co-cultured with infected BM-DC's. Taken together, these data suggest that Rv1818c-induced apoptotic signaling is likely regulated in part by the Smac-dependent activation of caspases in T cells. 相似文献
88.
Ramprasad OG Srinivas G Rao KS Joshi P Thiery JP Dufour S Pande G 《Cell motility and the cytoskeleton》2007,64(3):199-216
The number and distribution of lipid molecules, including cholesterol in particular, in the plasma membrane, may play a key role in regulating several physiological processes in cells. We investigated the role of membrane cholesterol in regulating cell shape, adhesion and motility. The acute depletion of cholesterol from the plasma membrane of cells that were well spread and motile on fibronectin caused the rounding of these cells and decreased their adhesion to and motility on fibronectin. These modifications were less pronounced in cells plated on laminin, vitronectin or plastic, indicating that cholesterol-mediated changes in adhesion and motility are more specific for adhesion mediated by fibronectin-specific integrins, such as alpha5beta1. These changes were accompanied by remodeling of the actin cytoskeleton, the spatial reorganization of paxillin in the membrane, and changes to the dynamics of alpha5 integrin and paxillin-rich focal adhesions. Levels of tyrosine phosphorylation at position 576/577 of FAK and Erk1/Erk2 MAP-kinase activity levels were both lower in cholesterol-depleted than in control cells. These levels normalized only on fibronectin when cholesterol was reincorporated into the cell membrane. Thus, membrane cholesterol content has a specific effect on certain signaling pathways specifically involved in regulating cell motility on fibronectin and organization of the actin cytoskeleton. 相似文献
89.
Cation-aromatic database 总被引:1,自引:0,他引:1
Cation-aromatic database (CAD) is a publicly available web-based database that aims to provide further understanding of interaction between a cation and the pi interactions. A tool to identify the interactions in a user-given protein is also added to the database. CAD is freely accessible via the Internet at http://203.199.182.73/gnsmmg/databases/cad/. 相似文献
90.
Wagner Sabrina Manickam Ravikumar Brotto Marco Tipparaju Srinivas M. 《Molecular and cellular biochemistry》2022,477(6):1829-1848
Molecular and Cellular Biochemistry - The nicotinamide adenine dinucleotide (NAD+) is an essential redox cofactor, involved in various physiological and molecular processes, including energy... 相似文献