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61.
Yi Sui Hai-Lu Zhao Heung Man Lee Jing Guan Lan He Fernand MM Lai Peter CY Tong Juliana CN Chan 《Translational oncology》2009,2(4):258-263
Nephrectomized rats have widely been used to study chronic renal failure. Interestingly, renal cell carcinoma occurred in the remnant kidney after uninephrectomy (UNX). In this study, we probed insulin-like growth factor (IGF)-1 signaling pathway in UNX-induced renal cancer. Adult male Sprague-Dawley rats were randomized into two groups: UNX rats (n = 22) and sham-operated rats (n = 12). Rats were killed at 3, 7, and 10 months. After 7 months after nephrectomy, the UNX rats developed renal cell carcinoma with increased expression of proliferating cell nuclear antigen, and 68.2% (15/22) of the animals exhibited invasive carcinoma. Western blot demonstrated significant down-regulation of IGF binding protein 3 contrasting with the up-regulation of protein kinase Cζ and Akt/protein kinase B in the renal cancer tissues. These findings indicate a unique rat model of UNX-induced renal cancer associated with enhanced IGF-1 signaling pathway. 相似文献
62.
Sefika C Mizrak Bart M Gadella Hatice Erdost Aytekin Ozer Ana MM van Pelt Federica MF van Dissel-Emiliani 《Reproductive biology and endocrinology : RB&E》2008,6(1):1-9
Background
The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction.Methods
In the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR (JAR cells), placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied on human placentae from normal (n = 3) and abnormal (n = 9) pregnancies.Results
Variations of transferrin expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In placentae from normal pregnancies, the expression of transferrin in the syncytium was significantly lower (p < 0.001) when compared to placentae from abnormal ones (gestational diabetes, pregnancy induced hypertension, drug abuse).Conclusion
These observations suggest that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placental dysfunction. 相似文献63.
West African dwarf nanny goats and their single-born kids were tested to find out their behavioural response to separation and their mutual recognition during the first 48 h post-partum. The tests were conducted at 18, 24, 36 or 48 h post partum. Chi-square analyses were used to determine whether age, sex and birth weight of kids as well as h partum and parity of dams had an effect on post-partum bahaviours.In a preliminary study where the kids were not prevented from sucking prior to the tests, the majority of kids (93%) and does (86%) exhibited apparant minimal concern to separation (i.e., had scores of less than 3) and also failed to seek each other. When the kids were prevented from sucking 2 h prior to the tests, the couple showed some response to separation and made attempts to seek each other. This suggests that in goats the state of the neonate's nourishment and the fullness of the dam's udder may be important factors that determine the willingness of the kid and the dam to seek each other when separated. The behavioural response of 48-h-old kids to separation from their dams when they had not sucked for 2 h was significantly higher (P<0.05) than that of 18-h-old kids. The sex and birth-weight of 48-h-old kids did not affect their response to separation from their dams. Hours post-partum and parity of does did not affect their response to separation from their kids even though the proportion of does exhibiting the highest response was much higher in primiparous then multiparous animals (44% vs. 13%). Recognition of dams by kids was poor at 18 and 24 h with more than half (71%) the kids failing to recognize their dams. Dam recognition ability improved with age and at 48 h the majority of kids (88%) were able to recognize their dams. Does tested at 48 h post partum had better (P0.05) kid recognition than those tested at 18 and 24 h. The sex and birth weight of kids and the parity of dams had no effect on the mutual recognition of kids and their dams at 48 h post-partum. 相似文献
64.
Andreas Krupke Wiebke Mohr Julie LaRoche Bernhard M Fuchs Rudolf I Amann Marcel MM Kuypers 《The ISME journal》2015,9(7):1635-1647
Symbiotic relationships between phytoplankton and N2-fixing microorganisms play a crucial role in marine ecosystems. The abundant and widespread unicellular cyanobacteria group A (UCYN-A) has recently been found to live symbiotically with a haptophyte. Here, we investigated the effect of nitrogen (N), phosphorus (P), iron (Fe) and Saharan dust additions on nitrogen (N2) fixation and primary production by the UCYN-A–haptophyte association in the subtropical eastern North Atlantic Ocean using nifH expression analysis and stable isotope incubations combined with single-cell measurements. N2 fixation by UCYN-A was stimulated by the addition of Fe and Saharan dust, although this was not reflected in the nifH expression. CO2 fixation by the haptophyte was stimulated by the addition of ammonium nitrate as well as Fe and Saharan dust. Intriguingly, the single-cell analysis using nanometer scale secondary ion mass spectrometry indicates that the increased CO2 fixation by the haptophyte in treatments without added fixed N is likely an indirect result of the positive effect of Fe and/or P on UCYN-A N2 fixation and the transfer of N2-derived N to the haptophyte. Our results reveal a direct linkage between the marine carbon and nitrogen cycles that is fuelled by the atmospheric deposition of dust. The comparison of single-cell rates suggests a tight coupling of nitrogen and carbon transfer that stays balanced even under changing nutrient regimes. However, it appears that the transfer of carbon from the haptophyte to UCYN-A requires a transfer of nitrogen from UCYN-A. This tight coupling indicates an obligate symbiosis of this globally important diazotrophic association. 相似文献
65.
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67.
Mirjafari H Farragher TM Verstappen SM Yates A Bunn D Marshall T Lunt M Symmons DP Bruce IN 《Arthritis research & therapy》2011,13(5):R159
Introduction
Cardiovascular disease (CVD) is the leading cause of death in patients with inflammatory polyarthritis (IP), especially in seropositive disease. In established rheumatoid arthritis (RA), insulin resistance (IR) is increased and associated with CVD. We investigated factors associated with IR in an inception cohort of patients with early IP. 相似文献68.
69.
Ebenezer?Addae Xiuli?Dong Eric?McCoy Chang?Yang Wei?Chen Liju?YangEmail author 《Journal of biological engineering》2014,8(1):11