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91.
92.
Hoque ME Arnett SD Lunte CE 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2005,827(1):51-57
Capillary electrophoresis (CE) has become a useful analytical tool for the analysis of microdialysis samples. However, CE with UV detection (CE-UV) does not provide detection limits sufficient to quantify glutathione (GSH) and glutathione disulfide (GSSG) in biological samples such as liver microdialysates, because of the small optical path length in the capillary. To overcome this limitation, an on-column preconcentration technique, pH-mediated base stacking, was used in this study to improve the sensitivity of CE-UV. This stacking technique allowed large volumes of high ionic strength sample injection without deterioration of the separation efficiency and resolution. A 26-fold increase in sensitivity was achieved for both GSH and GSSG using the pH-mediated base stacking, relative to normal injection without stacking. The limit of detection for GSH and GSSG was found to be 0.75 microM (S/N=6) and 0.25 microM (S/N=6), respectively. The developed method was used to analyze GSH and GSSG in liver microdialysates of anesthetized Sprague Dawley male rats. The basal concentrations of GSH and GSSG in the liver microdialysates of male rats were found to be 4.73+/-2.08 microM (n=7) and 5.52+/-3.66 microM (n=7), respectively. 相似文献
93.
The ovarian cycle ofRana tigrina was analysed by quantifying the developing oocytes (classified into stages on the basis of diameter) and atretic ones at
monthly intervals. Stages I to IV represent oocytes in the first growth phase and the remaining ones the vitellogenic or second
growth phase. Stages I–III occurred year round but exhibited significant variation in their number. The number of stage II
oocytes always dominated the other stages. Recruitment of oocytes to stages IV and V in April marked the initiation of vitellogenic
growth in all specimens. Of the 30 to 35% second growth phase oocytes, 25 to 28% reached ovulatory sizes by June. After spawning
the ovarian mass declined drastically from 15 to 0.2% of body mass in July. Atresia was maximal (5%) in August. In other months,
it was less than 1.5% of the total oocytes. Oogenic episodes occurred in March and July yielding new oocytes.
The number of first growth phase oocytes fluctuated from 65 to 95%. The fluctuation was inversely correlated with the second
growth phase oocytes indicating a 30 to 35% annual turnover rate of oocytes in the frog. The final egg number/ovarian mass
is positively correlated with the snout-vent length as well as body mass of the frogs.R. tigrina produces about 4000 eggs/100g body mass. Further, the mean number of yolky eggs/100 g body mass and the total volume (V) of eggs/frog were highly correlated.
Frogs living in captivity produced fewer eggs compared to the wild ones (3594 ± 227 in captivevs 4704 ± 317 in wild frogs). Also, these frogs failed to breed though they showed amplexus with breeding males. Injection of
desoxycorticosterone acetate however induced spawning in 4 out of 5 frogs. They released about 3000 eggs each. Captivity seems
to mainly impair breeding and to a little extent the vitellogenic growth of oocytes inR. tigrina. 相似文献
94.
C. R. Ahsan M. M. Hoque Z. Rasul M. M. Hoq 《World journal of microbiology & biotechnology》1992,8(2):187-189
Fourteen strains ofVibrio furnissii, isolated from different ulcerated areas of eel, were tested to check their enterotoxicity in an animal model. Most strains caused fluid accumulation in ileal loop tests after serial passages and culture filtrates of most of the strains caused induration and increase in vascular permeability in rabbit skin. Production of extracellular haemolysin was also detected in all the culture filtrates. All of these observations clearly establish the enterotoxicity of these organisms. 相似文献
95.
Stanley?G.?Kimani Sushil?Kumar Viralkumar?Davra Yun-Juan?Chang Canan?Kasikara Ke?Geng Wen-I?Tsou Shenyan?Wang Mainul?Hoque Andrej?Bohá? Anita?Lewis-Antes Mariana?S.?De Lorenzo Sergei?V.?Kotenko Raymond?B.?BirgeEmail author 《Cell communication and signaling : CCS》2016,14(1):19
Background
Tyro3, Axl, and Mertk (TAMs) are a family of three conserved receptor tyrosine kinases that have pleiotropic roles in innate immunity and homeostasis and when overexpressed in cancer cells can drive tumorigenesis.Methods
In the present study, we engineered EGFR/TAM chimeric receptors (EGFR/Tyro3, EGFR/Axl, and EGF/Mertk) with the goals to interrogate post-receptor functions of TAMs, and query whether TAMs have unique or overlapping post-receptor activation profiles. Stable expression of EGFR/TAMs in EGFR-deficient CHO cells afforded robust EGF inducible TAM receptor phosphorylation and activation of downstream signaling.Results
Using a series of unbiased screening approaches, that include kinome-view analysis, phosphor-arrays, RNAseq/GSEA analysis, as well as cell biological and in vivo readouts, we provide evidence that each TAM has unique post-receptor signaling platforms and identify an intrinsic role for Axl that impinges on cell motility and invasion compared to Tyro3 and Mertk.Conclusion
These studies demonstrate that TAM show unique post-receptor signatures that impinge on distinct gene expression profiles and tumorigenic outcomes.96.
97.
98.
Sheikh Imranudin Sheikh-Ali Akil Ahmad Siti-Hamidah Mohd-Setapar Zainul Akmal Zakaria Norfahana Abdul-Talib Aidee Kamal Khamis Md Enamul Hoque 《Journal of microbiology (Seoul, Korea)》2014,52(10):807-818
The contamination of food and feed by Aspergillus has become a global issue with a significant worldwide economic impact. The growth of Aspergillus is unfavourable to the development of food and feed industries, where the problems happen mostly due to the presence of mycotoxins, which is a toxic metabolite secreted by most Aspergillus groups. Moreover, fungi can produce spores that cause diseases, such as allergies and asthma, especially to human beings. High temperature, high moisture, retarded crops, and poor food storage conditions encourage the growth of mold, as well as the development of mycotoxins. A variety of chemical, biological, and physical strategies have been developed to control the production of mycotoxins. A biological approach, using a mixed culture comprised of Saccharomyces cerevisiae and Lactobacillus rhamnosus resulted in the inhibition of the growth of fungi when inoculated into fermented food. The results reveal that the mixed culture has a higher potential (37.08%) to inhibit the growth of Aspergillus flavus (producer of Aflatoxin) compared to either single culture, L. rhamnosus NRRL B-442 and S. cerevisiae, which inhibit the growth by 63.07% and 64.24%, respectively. 相似文献
99.
Md. Tozammel Hoque 《Biochemical and biophysical research communications》2009,379(1):60-64
Multidrug resistance protein 4 (MRP4/ABCC4), a member of the ATP-binding cassette protein superfamily, confers resistance to nucleoside and nucleotide analogs as well as camptothecin derivatives. MRP4 also mediates the efflux of certain cyclic nucleotides, eicosanoids, conjugated steroids, and uric acid. Depending on the cell type, MRP4 may localize to either apical or basolateral membranes in polarized cells. The adaptor protein NHERF1 has previously been implicated in MRP4 internalization in non-polarized cells. We have now found that NHERF1 levels are very low in polarized MDCKI cells which express MRP4 on basolateral membranes relative to polarized LLC-PK1 cells which express MRP4 on apical membranes. Furthermore, ectopic expression of FLAG-tagged NHERF1 in MDCKI cells and in MDCKI cells stably expressing eGFP-tagged MRP4 causes endogenous MRP4 and eGFP-MRP4, respectively, to traffic to the apical membranes. These data establish NHERF1 as a major determinant of MRP4 trafficking to apical membranes of mammalian kidney cells. 相似文献
100.
Tiana Baqueiro Momtchilo Russo Virgínia MG Silva Thayna Meirelles Pablo RS Oliveira Eliane Gomes Renato Barboza Ana T Cerqueira-Lima Camila A Figueiredo Lain Pontes-de-Carvalho Neuza M Alcantara-Neves 《Respiratory research》2010,11(1):51