Über ein vereinfachtes Verfahren der Gewinnung von Gurkensamen

Ohne ZusammenfassungMit 1 Abbildung.Quedlinburger Beiträge zur Züchtungsforschung Nr. 4.     

Über das Auftreten der Milchsäure in höheren Pflanzen,insbesondere Während der Keimung

Ohne ZusammenfassungMit 7 Textabbildungen.D 15.     

Observations on the morphology of submaxillary gland cells isolated in a density gradient,and the demonstration of interacinar bridges

Summary Methods are presented for the recovery of separate fractions of rabbit submaxillary acinar and striated duct cells from acini and duct segments, by means of density gradient centrifugation in glycerol-sucrose mixtures.Incomplete acinar fragmentation discloses the presence of acellular, nonstaining interacinar structures, referred to as interacinar bridges. The effectiveness of trypsin in liberating the acinar cells is ascribed to the disruption of intercellular secretory capillaries and interacinar bridges.The basal processes of the isolated striated duct cells correspond with both the striations observed with optical microscopy and the infolded cytoplasmic compartments revealed with electron microscopy. The presence of clearly discernible spaces between the basal processes is compatible with the concept of intermembrane dilations.     

Über die Biologische Wirksamkeit einiger Gibberellinsäure-Abbauprodukte

Zusammenfassung Die biologische Wirksamkeit von 4 Gibberellinsäure-Abbauprodukten (Abb. 1) wurde an mehreren Testpflanzen unter Verwendung von A₃ als Standard vergleichend untersucht. Die Substanzen wurden dünnschichtchromatographisch gereinigt und auf Einheitlichkeit geprüft. Gibberellensäure. Die Mais-Zwergmutanten d-1, d-3 und d-5 reagierten auf Mindestdosen von l g/Pflanze. Im Zwergerbsen-und Reissämlingstest zeigte sich geringere, im Lactuca-Hypokotyltest sehr schwache Wirksamkeit. Die Dunkelkeimung von Lactuca-Achänen wurde nicht stimuliert.Dicarbonsäure A (s. Material und Methodik). Bei Zea mays d-5 besaß die Verbindung etwa gleichstarke, bei d-1 und d-3 etwas geringere Aktivität als Gibberellensäure. Bei Erbsen und im Lactuca-Hypokotyltest wurde nur schwache, bei Reissämlingen praktisch keine Wirksamkeit festgestellt. Allogibberinsäure war bei Lactuca sativa (Hypokotylverlängerung und Dunkelkeimung) und Oryza sativa stärker wirksam als die übrigen Abbauprodukte. Die Maismutante d-5 reagierte ebenfalls relativ empfindlich; bei d-3 und d-1 zeigte sich geringere, bei Zwergerbsen nur sehr schwache Aktivität. Gibberinsäure besaß lediglich bei Zea mays d-5 in hohen Dosen (100 g/Pflanze) eine geringe Wirksamkeit.

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On the biological activity of some degradation products of gibberellic acid. Gibberellins. Part VIII

Summary The potency of four degradation products of gibberellic acid (Fig.1) was studied in several bioassays using gibberellin A₃ as a standard. The substances had been purified and checked for homogeneity by means of thin-layer chromatography. Gibberellenic acid. Dwarf maize mutants d-1, d-3 and d-5 showed positive reactions after application of minimal doses of 1 g per plant. In the case of dwarf peas and rice seedlings gibberellenic acid exhibited a lower potency, and with Lactuca seedlings (hypocotyl extension) very low biological activity was observed. Germination of Lactuca seeds in the dark was not stimulated.Dicarbonic acid A (see Material und Methodik) had the same potency as gibberellenic acid with Zea mays d-5 and slightly lower potency with d-1 and d-3. With dwarf peas as well as in the Lactuca hypocotyl test dicarbonic acid A exhibited only low activity and in the case of rice seedlings the activity was negligible. Allogibberic acid had a higher potency than the other degradation products in bioassays with Lactuca sativa (both hypocotyl extension and seed germination) and with Oryza sativa. Zea mays d-5 was also relatively sensitive towards this substance. With d-1 and d-3 allogibberic acid exhibited a lower potency and with Pisum sativum only a very low potency. Gibberic acid. Only Zea mays d-5 showed positive reactions after application of hig doses (100 g per plant).

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VII. Mitt.: Sembdner u. Schreiber (1965b).     

Discovery of a novel species,Theileria haneyi n. sp., infective to equids,highlights exceptional genomic diversity within the genus Theileria: implications for apicomplexan parasite surveillance

A novel apicomplexan parasite was serendipitously discovered in horses at the United States – Mexico border. Phylogenetic analysis based on 18S rDNA showed the erythrocyte-infective parasite to be related to, but distinct from, Theileria spp. in Africa, the most similar taxa being Theileria spp. from waterbuck and mountain zebra. The degree of sequence variability observed at the 18S rDNA locus also suggests the likely existence of additional cryptic species. Among described species, the genome of this novel equid Theileria parasite is most similar to that of Theileria equi, also a pathogen of horses. The estimated divergence time between the new Theileria sp. and T. equi, based on genomic sequence data, is greater than 33?million years. Average protein sequence divergence between them, at 23%, is greater than that of Theileria parva and Theileria annulata proteins, which is 18%. The latter two represent highly virulent Theileria spp. of domestic cattle, as well as of African and Asian wild buffalo, respectively, which differ markedly in pathology, host cell tropism, tick vector and geographical distribution. The extent of genome-wide sequence divergence, as well as significant morphological differences, relative to T. equi justify the classification of Theileria sp. as a new taxon. Despite the overall genomic divergence, the nine member equi merozoite antigen (EMA) superfamily, previously found as a multigene family only in T. equi, is also present in the novel parasite. Practically, significant sequence divergence in antigenic loci resulted in this undescribed Theileria sp. not being detectable using currently available diagnostic tests. Discovery of this novel species infective to equids highlights exceptional diversity within the genus Theileria, a finding with serious implications for apicomplexan parasite surveillance.