胺碘酮诱发甲状腺功能亢进症32例临床分析

目的:探讨胺碘酮诱发的甲状腺功能亢进症(AIT)的临床进程和预后因素.方法:回顾性分析我院32例有完整资料的胺碘酮诱发的甲状腺功能亢进症患者,对其,临床特征、实验室参数、治疗方法和随访过程中的事件发生率进行评估,以和AIT相关的并发症如:死亡、心衰住院、中风、心律失常等作为观察终点.结果:32例病人中有1l例接受强的松治疗,但血清游离甲状腺素恢复正常的时间和非强的松治疗组比较没有差异,长期随访结果显示强的松治疗组具有较高的事件发生率.结论:胺碘酮诱发甲状腺亢进症具有较高的事件发生率,强的松治疗不能缩短血清甲状腺素恢复正常时间,反而增加不良事件发生率.     

小蓬草精油对两种蚊虫的毒杀活性和成分分析

【目的】明确小蓬草Conyza canadensis ( L.) Cronq.精油的杀虫潜力及其活性成分。【方法】通过浸虫法和密闭熏蒸法测试了小蓬草精油对白纹伊蚊Aedes albopictus和致倦库蚊Culex pipiens quinquefasciatus幼虫及成蚊的毒杀活性, 并利用气相色谱 质谱联用仪（GC-MS）对精油的挥发性成分进行了定性分析。【结果】 小蓬草精油对白纹伊蚊1-4龄期幼虫及蛹的24 h LC50值分别为25.01, 45.88, 56.94, 64.60和346.23 μg/mL; 对致倦库蚊幼虫1-4龄期幼虫及蛹的24 h LC₅₀值分别为9.16, 8.65, 32.12, 43.68和197.83 μg/mL。在剂量分别为48, 64, 80, 96, 112和128 μg/cm3时, 小蓬草精油对白纹伊蚊成蚊的KT₅₀值分别为28.81, 22.31, 20.38, 17.05, 13.92和9.74 min; 对致倦库蚊的KT50值分别为34.90, 32.97, 23.97, 19.60, 15.20和10.34 min。 24 h熏蒸对白纹伊蚊和致倦库蚊成蚊的LC50值分别是75.46和99.19 μg/cm³。小蓬草精油的GC MS定性分析共分离鉴定出31种化合物, 其中单萜类物质6种, 倍半萜烯类物质17种, 含氧萜烯类6种。【结论】结果表明小蓬草精油对这两种蚊虫的毒杀活性较高, 具有深开发潜力。     

黑果枸杞花青素结构差异对其稳定性及细胞抗氧化活性的影响

本文研究了黑果枸杞花青素化合物结构对其稳定性和细胞抗氧化活性的影响。通过半制备型HPLC分离纯化得到了4种黑果枸杞花青素化合物并鉴定结构,利用比色法评价了化合物的稳定性、细胞毒实验以及H₂O₂诱导的SH-SY5Y细胞损伤模型评价了细胞抗氧化活性,并讨论了化学结构对其影响。结果表明反式结构比顺式结构在中性和弱碱性条件下有更好的稳定性,反式结构、糖基的增加以及母核B环上的甲氧基取代均可以通过缩短母核与糖链的分子距离从而提高稳定性,糖基的增加能显著地降低损伤细胞中的乳酸脱氢酶水平,且反式结构较顺式结构更有活性优势。综上所述,黑果枸杞花青素的结构影响稳定性和细胞抗氧化活性,但糖基的增加和酰基的反式异构对二者有更积极的意义。     

胸腺素α原体外诱导小鼠胸腺细胞凋亡的初步探讨

胸腺细胞经ProTα和/或氢化考的松处理以后,采用PI染色法检测亚二倍体细胞百分率、荧光光度计检测细胞内游离Ca~(2 )浓度及琼脂糖凝胶电泳定性检验DNA片段化。结果发现单独或与氢化考的松联合应用,ProTα均可明显促进DNA片断化、提高亚二倍体细胞百分率并且也明显提高细胞内游离Ca~(2 )浓度。本实验说明ProTα能促进胸腺细胞的凋亡。     

红细胞的前向光散射

本文从红细胞前向光散射的观点出发对红细胞的尺寸、红细胞的变形性研究以及红细胞容积、血红蛋白浓度等参数的测定作了系统的思考。提出在红细胞与周围悬浮介质折射车相差不大时,反常衍射比夫朗和费衍射更适合用于红细胞前向光散射的研究。利用两组不同角间隔的前向散射光来同时测量红细胞容积和血红蛋白浓度。同时其它一些标识红细胞的参数如平均红细胞容量（ＭＣＶ）、平均血红蛋白量（ＭＣＨ）等均可直接或间接由这两项参数导出。最后还将红细胞的光散射与Ｍｉｅ理论作了对比．     

新型隐球酵母非编码小RNAs的研究进展

新型隐球酵母是一种担子菌病原真菌,主要感染免疫功能缺陷的人群,例如HIV-1感染病人,最终会引起致命隐球菌性脑膜炎。非编码小RNAs一般指长度为20-30nt的小RNAs,具有调节功能。新型隐球酵母能够产生大量的小RNAs,但是其生成（biogenesis）过程以及生物学功能尚未完全阐述。本文就新型隐球酵母中小RNAs的特征和产生、以及在新型隐球酵母中的生物学作用和机制进行综述。     

Language Differences in the Brain Network for Reading in Naturalistic Story Reading and Lexical Decision

Differences in how writing systems represent language raise important questions about whether there could be a universal functional architecture for reading across languages. In order to study potential language differences in the neural networks that support reading skill, we collected fMRI data from readers of alphabetic (English) and morpho-syllabic (Chinese) writing systems during two reading tasks. In one, participants read short stories under conditions that approximate natural reading, and in the other, participants decided whether individual stimuli were real words or not. Prior work comparing these two writing systems has overwhelmingly used meta-linguistic tasks, generally supporting the conclusion that the reading system is organized differently for skilled readers of Chinese and English. We observed that language differences in the reading network were greatly dependent on task. In lexical decision, a pattern consistent with prior research was observed in which the Middle Frontal Gyrus (MFG) and right Fusiform Gyrus (rFFG) were more active for Chinese than for English, whereas the posterior temporal sulcus was more active for English than for Chinese. We found a very different pattern of language effects in a naturalistic reading paradigm, during which significant differences were only observed in visual regions not typically considered specific to the reading network, and the middle temporal gyrus, which is thought to be important for direct mapping of orthography to semantics. Indeed, in areas that are often discussed as supporting distinct cognitive or linguistic functions between the two languages, we observed interaction. Specifically, language differences were most pronounced in MFG and rFFG during the lexical decision task, whereas no language differences were observed in these areas during silent reading of text for comprehension.     

一株生淀粉酶产生菌的分离鉴定及酶学性质研究

采用苔酚蓝-生淀粉法从贵州遵义百年磨坊的磨盘下的土样中筛选得到一株具有生淀粉降解能力的丝状真菌,经形态学鉴定和ITS核苷酸序列比对确定该菌为Rhizopus microsporusvar.chinensis。本文首次报道了由Rhizopus microsporus var.chinensis产生的生淀粉酶。通过酶学性质研究发现,该菌株所产生淀粉酶拥有较好的温度耐受性和较宽的pH适用范围,具有一定的工业应用价值。     

数据噪音构建基因布尔网络模型的方法

在分析基因数据时,往往有噪音出现,因此借用基因表达谱数据中的噪音来建立卡诺图,可以得到布尔网络逻辑函数。而且利用此方法确定蛋白质与蛋白质之间的逻辑关系,建立蛋白质的逻辑网络。通过该方法可以寻找直系同源簇蛋白质数据的逻辑关系。