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A revision of the Encarsia pergandiella species complex (Hymenoptera: Aphelinidae) shows cryptic diversity in parasitoids of whitefly pests
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MARCO GEBIOLA MAURILIA M. MONTI ROISHENE C. JOHNSON JAMES B. WOOLLEY MARTHA S. HUNTER MASSIMO GIORGINI PAOLO A. PEDATA 《Systematic Entomology》2017,42(1):31-59
Encarsia pergandiella Howard, described from North America (USA), and Encarsia tabacivora Viggiani, described from South America (Brazil) (Hymenoptera: Aphelinidae), are two formally recognized taxonomic entities, that have been treated by several authors as synonyms due to lack of strong diagnostic characters. Taxonomy of these species is further complicated because several populations, geographically separated and differing in their biology, have been included under the concept of E. pergandiella. Among these, a population originally collected in Brazil and introduced to North America reproduces by thelytokous parthenogenesis and is infected by the symbiont Cardinium, while a morphologically indistinguishable population, naturally occurring in Texas, is biparental and infected by a related strain of Cardinium that induces cytoplasmic incompatibility. A third population known from California and introduced to the Old World is biparental and uninfected by intracellular symbionts. While adult females of the first two populations have entirely light yellow bodies and pupate face up (light form), those of the third population have largely brown bodies and pupate face down (dark form). Other dark form populations are known from Texas, Florida and New York. Because these parasitoids are economically important biological control agents of cosmopolitan whitefly pests, it is critical to characterize them correctly. In this study, we integrated molecular and morphometric analyses to substantiate observed differences in biological traits, and resolve the complicated taxonomy of this species complex. We sequenced the mitochondrial cytochrome c oxidase subunit I gene and the D2 region of the ribosomal 28S gene for individuals of both light form (from Texas and Brazil) and dark form (from California, Texas, Italy and Canary Islands) originating from laboratory cultures or collected in the field. Phylogenetic analysis unambiguously distinguished three well‐supported groups corresponding to the Texas light form, the Brazil light form and the dark form. Individuals of these three groups, in combination with all available type material (E. pergandiella, its synonym Encarsia versicolor Girault and E. tabacivora) and additional museum specimens of the dark form from New York and Italy, were subjected to multivariate morphometric analyses using Burnaby principal component analysis followed by a linear discriminant analysis, and multivariate ratio analysis. Overall, the analyses showed that: (i) E. pergandiella and E. tabacivora are two distinct species; (ii) the thelytokous Brazil light form corresponds to E. tabacivora; (iii) the biparental Texas light form is a new species formally described here as Encarsia suzannae sp.n. ; (iv) two new biparental species can be referred to the dark form, one described as Encarsia gennaroi sp.n. including the populations sampled in California, Texas, Italy and Canary Islands, and the other corresponding to the population from New York described as Encarsia marthae sp.n. A dichotomous key for both sexes of the species of the E. pergandiella complex is provided for identification. This published work has been registered in ZooBank, http://zoobank.org/urn:lsid:zoobank.org:pub:278475A0‐C2C4‐4400‐A042‐A5716457829D . 相似文献
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ELISA BIGLIARDI MARIA GLORIA SELMI PIETRO LUPETTI SILVIA CORONA SIMONETTA GATTI MASSIMO SCAGLIA LUCIANO SACCHI 《The Journal of eukaryotic microbiology》1996,43(3):181-186
A study of the spore wall of Encephalitozoon hellem was performed on thin sections, freeze-fracture, and deep-etched samples to obtain information on spore wall organization and composition. Our observations demonstrate that the spore wall is formed by an inner 30–35 nm electron-lucent endospore and an outer 25–30 nm electron-dense exospore. The exospore is a complex of three layers: an outer spiny layer, an electron-lucent intermediate lamina and an inner fibrous layer. Freeze-fracture and deep-etching techniques reveal that the intermediate lamina and the inner fibrous layer result from the different spatial disposition of the same 4-nm thick fibrils. In thin sections the endospore reveals a scattered electron-dense material that appears in the form of trabecular structures when analyzed in deep-etched samples. The presence of chitin in the exospore is discussed. 相似文献
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ILARIA BRUNI ANDREA GALIMBERTI SERGIO SGORBATI MAURIZIO CASIRAGHI MASSIMO LABRA 《Botanical journal of the Linnean Society. Linnean Society of London》2012,169(3):518-529
Vegetation surveys have a long tradition in ecological studies, but several limitations in the morphological identification of species have been recognized. The objective of this study was to evaluate the effectiveness of DNA barcoding in plant species identification to save field technicians time and resources. Vegetation surveys were performed in four plots of semi‐dry grassland in the Italian subalpine region of Lombardy. Two identification approaches were employed: a conventional morphological identification and a molecular multi‐marker DNA barcoding method. Results showed that morphological identification of 49 species collected from the study area (five field inspections) required a substantial amount of time to complete relative to the molecular method. The same 49 samples were analysed using the following DNA multi‐marker barcodes: rbcL, matK and trnH‐psbA. rbcL showed 100% amplification success with standard primers, but low interspecific genetic variability. matK demonstrated some amplification problems with standard primers; however, consistent genetic diversity was observed. Finally, the trnH‐psbA spacer region exhibited reliable amplification success and the highest molecular variability. In a comparison with publicly available databases, trnH‐psbA and matK returned the highest proportion of identified samples, whereas rbcL returned several misidentifications. The DNA barcoding approach is a powerful tool in vegetation surveys and may significantly reduce the time and cost spent for species identification. However, to effectively apply DNA barcoding in vegetation surveys, exhaustive local or regional molecular databases must be defined. © 2012 The Linnean Society of London, Botanical Journal of the Linnean Society, 2012, 169 , 518–529. 相似文献
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DANIELE SALVI MASSIMO CAPULA PIERLUIGI BOMBI MARCO A. BOLOGNA 《Biological journal of the Linnean Society. Linnean Society of London》2009,98(3):661-676
The peculiar bioclimatic and geographic features of Corso–Sardinian islands may provide an ideal scenario for investigating microevolutionary processes, given their large heterogeneity of environments, which could affect dispersal and gene flow among populations, as well as processes of local adaptation. The genetic variation and differentiation among populations of the endemic lizard Archaeolacerta bedriagae were studied by allozyme electrophoresis at 20 presumptive loci. The genetic structure of this species is characterized by relatively high levels of polymorphism and low differentiation among populations. The pattern of genetic differentiation cannot be explained by genetic drift as a function of geographic distance. Genetic distance data show that genetic variation is distributed into three geographically coherent population groups and suggest a recent (Late Pleistocene) origin for the observed geographic fragmentation. The analysis of environmental correlates of allozymic variation indicates a strong correlation of the Idh-1 locus with climatic variables. The frequency of the Idh-1106 allele is negatively correlated with annual temperature, and positively correlated with annual precipitation. In addition, the observed heterozygosity at this locus decreases towards more arid climatic regimes. The results obtained support the assumption of differential selection acting on Idh-1 allozymes under diverse climates. An association between Idh-1 allozymes and local bioclimatic regimes was also observed for the sympatric lizard Podarcis tiliguerta , suggesting a key role for such selective agents on Idh-1 polymorphism in these two Corso–Sardinian lacertids. © 2009 The Linnean Society of London, Biological Journal of the Linnean Society , 2009, 98 , 661–676. 相似文献
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SEBASTIEN DELARBRE SIMONETTA GATTI MASSIMO SCAGLIA MICHEL DRANCOURT 《The Journal of eukaryotic microbiology》2001,48(4):471-474
Encephalitozoon hellem is a microsporidian species responsible for opportunistic infections in AIDS patients. Use of a novel chitinase-based method allowed unsheared chromosomal DNA to be recovered from eleven E. hellem isolates derived from three geographic regions. All isolates were typed by 18S rDNA sequencing, which showed that they belonged to intemal transcribed spacer type 1. After ethidium bromide staining, pulsed-field gel electrophoresis (PFGE) analysis discriminated two new karyotypes comprising 7 and 8 chromosomal bands respectively, ranging in size from 205- to 272-kb pairs. Genomic size was estimated to be 2.39 Mb. Our data indicate PFGE is useful for typing E. hellem and confirms genetic diversity among E. hellem genotypes. 相似文献
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