THE EFFECT OF THE TETRACYCLINE COMPOUNDS ON THE STORAGE LIFE AND MICROBIOLOGY OF CHILLED EVISCERATED POULTRY

SUMMARY: Chlortetracycline, oxytetracycline and tetracycline, incorporated at 10 p/m in the ice slush used for cooling, were equally efficient in extending the storage life of eviscerated poultry which was subsequently frozen, thawed and stored at 1°. There was complete correlation between the sensory assessment of 'off'odour and the total numbers of micro-organisms present when these were determined by taking bulk samples of skin from the area under the wing and of the surface tissue of the visceral cavity near the vent. Direct microscopical counts confirmed that the latter area was the most heavily contaminated part of the visceral cavity. Non-pigmented strains of Pseudomonas were the main spoilage organisms of untreated chickens but Achromobacter strains and yeasts were responsible for the spoilage of the chlortetracycline treated chickens. Fluorescence was only observed in spoiled chickens where pigmented strains of Pseudomonas occurred in significant numbers. Spoilage in both control and antibiotic treated poultry was accompanied by a rise in pH in the contaminated muscles, but for various reasons this change could not be used as a direct measure of the extent of the microbial contamination.     

EXPERIMENTS ON THE AIRTIGHT STORAGE OF DAMP GRAIN

Airtight storage as a means of preventing deterioration of damp grain was studied both on a laboratory scale and in 10-ton bins, at grain moisture contents from 17 to 24%.
Except when containers leaked, there was no development of mould, and the grain was bright and free-flowing, even after prolonged storage, at high moisture content. The grain remained mould-free after a bin was opened several times and small quantities of grain run out in cool weather. Such grain, removed from hermetic conditions and stored in sacks in an unheated building, showed no mould development for several weeks.
With prolonged hermetic storage or at high moisture content the grain developed a sour-sweet smell and taste which when extreme were not entirely removed by subsequent airing or drying.
There was no spontaneous heating; the grain in the 10-ton bins reflected the mean temperature of the surroundings.
The oxygen in the intergranular air was reduced to a low level and replaced by carbon dioxide within a few days or weeks, according to the moisture content and temperature of the grain. At moisture contents of 16% and above, appreciable positive pressure developed inside the containers. The carbon dioxide concentration rose to 90–95% at grain moistures of 22–24%, 70–75% at 19%, 50% at 18%, and 35–40% at about 17% moisture content. The change from aerobic to anaerobic activity was marked by a reduction in the rate of production of carbon dioxide. With grain of less than 14% moisture content, the rate of respiration was slow, the carbon dioxide concentration being only about 2% after 18 months' storage. As long as oxygen remained, the apparent respiratory quotient was consistently between 0.6 and 0.7, whatever the moisture content of the grain.
This work formed part of the programme of work of the Pest Infestation Laboratory.     

THE INCIDENCE OF BACTERIUM COLI IN FARM WATER SUPPLIES

SUMMARY: Farm water supplies giving 37° positives in MacConkey's broth within 24 hr. had a much higher incidence of presumptive Bact. coli type I than those which gave 37° positives during the second day of incubation only; presumptive Bact. coli reactions were obtained with 85% of the 7,522 tubes positive in 24 hr. compared with 23% for the 7,593 tubes showing positive reactions during the second day at 37°.     

Fat Metabolism in Germinating Citrullus vulgaris. Part II

Breakdown of fat in watermelon seedlings germinated in the darkoccurs in essentially the same way as in the light. Free fattyacids do not accumulate and the composition of the lipid remainsalmost unchanged during its rapid utilization. The low weight of ‘protoplasmic lipid’ remainingin the etiolated cotyledons after the storage fat has disappearedcontains a lower proportion of linolenic acid than that fromgreen cotyledons of a corresponding age. The weight of phosphatide (lipid P) per cotyledon increases both in light- anddark-grown seedlings until 8 days, thereafter declining in thedark but continuing to rise in the light. Phosphatide and glyceridefractions from the same fat resemble each other fairly closelyin fatty acid composition.     

Fatty Acids in Chloroplasts and Leaves

The fatty acid composition of green and white leaf tissue inAcer negundo, Zea mais, and Ilex aquifolium, of green and yellowtissue in Ligustrum ovatifolium and of etiolated and green tissuein Vicia faba has been determined. The mesophytic green leavesexamined show a general similarity in fatty acid composition,characterized by a high concentration of non-conjugated octadecatrienoicacid. Chloroplasts were isolated from Vicia and Acer and containan even higher concentration of this acid and only traces ofnon-conjugated octadecadienoic acid. Conjugated diene and trieneacids occur in traces in chioroplasts, but are also found innon-green leaf tissue. The fats of non-green leaves are in generalmore saturated than those from green tissue but vary considerablyin composition. The relationship between fat composition andplastid development is discussed.     

Evolution and phylogeny of the scarab subtribe Anisopliina (Coleoptera: Scarabaeidae: Rutelinae: Anomalini)

Abstract.  The subtribe Anisopliina (Scarabaeidae: Rutelinae: Anomalini) is associated with grasses, and its species are distributed in the Palaearctic, Oriental, Ethiopian, Nearctic and Neotropical biogeographical regions. Phylogenetic analysis of adult morphological characters was conducted to examine the monophyly and classification of the group, as well as to examine characters associated with grass pollinivory and graminivory. We review the biology, phylogeny and classification of the Anisopliina and provide an overview of each genus. The analysis of ninety-one morphological characters using parsimony does not support the monophyly of the subtribe Anisopliina. Instead, the results provide support for a group referred to here as the anisopliine clade, a circum-Mediterranean group, forming an internal clade within the well-supported tribe Anomalini. Sister group relationships are discussed, possibly being associated with a New World anomaline taxon. Character states associated with grass herbivory, including mouthpart and leg characters, are discussed based on the phylogenetic analysis. Within the Anomalini, an evolutionary shift from generalized leaf feeding to grass associations and grass pollen feeding is supported.