Coordinated approaches to quantify long‐term ecosystem dynamics in response to global change

Many serious ecosystem consequences of climate change will take decades or even centuries to emerge. Long‐term ecological responses to global change are strongly regulated by slow processes, such as changes in species composition, carbon dynamics in soil and by long‐lived plants, and accumulation of nutrient capitals. Understanding and predicting these processes require experiments on decadal time scales. But decadal experiments by themselves may not be adequate because many of the slow processes have characteristic time scales much longer than experiments can be maintained. This article promotes a coordinated approach that combines long‐term, large‐scale global change experiments with process studies and modeling. Long‐term global change manipulative experiments, especially in high‐priority ecosystems such as tropical forests and high‐latitude regions, are essential to maximize information gain concerning future states of the earth system. The long‐term experiments should be conducted in tandem with complementary process studies, such as those using model ecosystems, species replacements, laboratory incubations, isotope tracers, and greenhouse facilities. Models are essential to assimilate data from long‐term experiments and process studies together with information from long‐term observations, surveys, and space‐for‐time studies along environmental and biological gradients. Future research programs with coordinated long‐term experiments, process studies, and modeling have the potential to be the most effective strategy to gain the best information on long‐term ecosystem dynamics in response to global change.     

Temperature cues phenological synchrony in ant‐mediated seed dispersal

Species‐specific climate responses within ecological communities may disrupt the synchrony of co‐evolved mutualisms that are based on the shared timing of seasonal events, such as seed dispersal by ants (myrmecochory). The spring phenology of plants and ants coincides with marked changes in temperature, light and moisture. We investigate how these environmental drivers influence both seed release by early and late spring woodland herb species, and initiation of spring foraging by seed‐dispersing ants. We pair experimental herbaceous transplants with artificial ant bait stations across north‐ and south‐facing slopes at two contrasting geographic locations. This use of space enables robust identification of plant fruiting and ant foraging cues, and the use of transplants permits us to assess plasticity in plant phenology. We find that warming temperatures act as the primary phenological cue for plant fruiting and ant foraging. Moreover, the plasticity in plant response across locations, despite transplants being from the same source, suggests a high degree of portability in the seed‐dispersing mutualism. However, we also find evidence for potential climate‐driven facilitative failure that may lead to phenological asynchrony. Specifically, at the location where the early flowering species (Hepatica nobilis) is decreasing in abundance and distribution, we find far fewer seed‐dispersing ants foraging during its fruit set than during that of the later flowering Hexastylis arifolia. Notably, the key seed disperser, Aphaenogaster rudis, fails to emerge during early fruit set at this location. At the second location, A. picea forages equally during early and late seed release. These results indicate that climate‐driven changes might shift species‐specific interactions in a plant–ant mutualism resulting in winners and losers within the myrmecochorous plant guild.     

In the Way of Development: Indigenous Peoples, Life Projects, and Globalization

In the Way of Development: Indigenous Peoples, Life Projects, and Globalization. Mario Blaser, Harvey A. Feit, and Glenn McRae, eds. New York: Zed Books, 2004. 362 pp.     

How useful are the recommended counts and indices in the systematics of the Octopodidae (Mollusca: Cephalopoda)

A morphological dataset based on 14 standard counts and indices was constructed for 68 specimens comprising 12 species of octopuses. This was used to construct distance matrices based on morphological characters. These matrices were compared with genetic distance matrices compiled during molecular phylogenetic analyses of the same 12 species using four mitochondrial and two nuclear genes. Mantel tests showed that there was significant congruence between the phenetic and genetic matrices, suggesting that the genetic signal is reflected in the morphological data set. Matrices of geographical distance were constructed for the 12 species based on the latitude, longitude, and depth of capture of 1726 individuals. These matrices never showed significant congruence with genetic data or with morphological data. Multivariate analysis of the morphological dataset suggests that these counts and indices, traditionally used for discriminating between species in cephalopods, do not show great discrimination at species level, but provide excellent discrimination at the generic level, and, as such, might be useful for resolving the generic placement of some problematic taxa. © 2008 The Linnean Society of London, Biological Journal of the Linnean Society, 2008, 95 , 205–218.     

Mitochondrial genomes in the Hymenoptera and their utility as phylogenetic markers

Abstract.  In this study, we assessed the ability of mitochondrial genome sequences to recover a test phylogeny of five hymenopteran taxa from which phylogenetic relationships are well accepted. Our analyses indicated that the test phylogeny was well recovered in all nucleotide Bayesian analyses when all the available holometabolan (i.e. outgroup) taxa were included, but only in Bayesian analyses excluding third codon positions when only the hymenopteran representatives and a single outgroup were included. This result suggests that taxon sampling of the outgroup might be as important as taxon sampling of the ingroup when recovering hymenopteran phylogenetic relationships using whole mitochondrial genomes. Parsimony analyses were more sensitive to both taxon sampling and the analytical model than Bayesian analyses, and analyses using the protein dataset did not recover the test phylogeny. In general, mitochondrial genomes did not resolve the position of the Hymenoptera within the Holometabola with confidence, suggesting that an increased taxon sampling, both within the Holometabola and among outgroups, is necessary.     

Revision of the Euthalia phemius complex (Lepidoptera: Nymphalidae) based on morphology and molecular analyses

Adults of the Euthalia phemius complex, which is composed of three South‐East Asian nymphalid species, Euthalia phemius, Euthalia ipona, and Euthalia euphemia, were genetically analysed by examining mitochondrial and nuclear genes. The E. phemius complex was also examined morphologically, with particular emphasis on wing markings and male genitalia. No significant differences amongst the three species in the complex were detected with respect to either genetic distance or genital morphology. We therefore conclude that the three currently recognized Euthalia species belong to a single species. Accordingly, E. ipona is synonymized with E. phemius. Euthalia euphemia is treated as a subspecies of E. phemius. Type specimens of all taxa and a synonymic list for the E. phemius complex are also given. In addition, we briefly discuss the evolution and biogeography of the species complex. © 2011 The Linnean Society of London, Zoological Journal of the Linnean Society, 2012, 164 , 304–327.     

Habitat attributes associated with short‐term settlement of Ozark hellbender (Cryptobranchus alleganiensis bishopi) salamanders following translocation to the wild

1. Organisms associated with lotic systems rank among the most threatened because of global change. Although translocation is being increasingly applied as a conservation strategy, most studies have focused on survival and recruitment of individuals, and few have attempted to identify how habitat attributes influence short‐term settlement of animals during the critical post‐release period. 2. We demonstrate the application of resource selection modelling in an information theoretic framework to identify release‐site characteristics that will increase the likelihood of settlement for a fully aquatic benthic stream salamander, the Ozark hellbender (Cryptobranchus alleganiensis bishopi). We fit discrete choice models using data from 29 radio‐tagged hellbenders that were translocated to two sites in the North Fork of the White River (NFWR), Missouri (U.S.A.). We defined resource availability at two spatial scales (stream reach and home range) and quantified abiotic habitat attributes at 3181 salamander locations and 6329 random available locations collected between May 2008 and August 2009. 3. At both sites and spatial scales, a single model received substantially greater support (0.96–1.00 of total model weight) than all other models, and top‐ranked models were similar in form and predictive ability. At both spatial scales, selection was positively influenced by the presence of cobble‐boulder substratum relative to bedrock and finer substrata. We also noted a negative interactive effect between distance to the nearest substratum particle large enough to provide cover (i.e. at least one axis ≥15 cm in length) and an increase in either a direct or relative (i.e. pool, run, and riffle) measure of water velocity. 4. Collectively, salamanders released in our study selected resources indicative of long‐term benthic microhabitat stability. However, despite strong selection of cobble‐boulder substratum, 8% (282 of 3181) of captive‐reared hellbender locations occurred in bank crevices and root masses. Although several studies have reported the importance of near bed hydraulics in determining occurrence of stream macroinvertebrates, our findings are the first to indicate that spacing among cobble‐boulder substrata may be important for hellbenders. 5. To increase the likelihood of short‐term settlement of captive‐reared hellbenders in the wild, we recommend prioritising release sites where the average distance between cobble‐boulder particles within habitat patches is minimised. In general, average spacing among cobble and boulder substrata should be <1 m in habitat patches where mean benthic water velocity exceeds 0.1 m s^?1, and <0.5 m where water velocity approaches 0.30 m s^?1. Based on home range sizes of captive‐reared Ozark hellbenders, the collective extent of suitable cobble‐boulder habitat patches within release sites should approximate at least 10 m² per salamander released.     

Responses of lotic periphyton to pulses of phosphorus: P‐flux controlled growth rate

1. The response of stream periphyton to the addition of limiting nutrients has been the focus of many studies. However, the influence of pulsed nutrient additions has not previously been examined. This study investigates the biomass accrual and physiological responses of phosphorus‐limited lotic periphyton to hourly phosphate fluxes. 2. Two pulsing experiments were conducted: (i) a variable flux trial that compared variable hourly P‐fluxes, delivered either continuously at different concentrations or at the same concentration but in pulses of differing duration per hour and (ii) a constant flux trial that compared periphyton responses at a set hourly P‐flux but delivered in pulses of varying concentration and duration. 3. Growth response and alkaline phosphatase activity during the variable flux experiment showed that periphyton responds to the hourly integrated flux of phosphorus, regardless of whether the nutrient is supplied in short concentrated pulses or continuously at much lower concentrations. 4. The constant flux experiment examined the pulse period required to attain maximum biomass for a given phosphorus flux. Periphyton response to 5‐min pulses of phosphate per hour approximated the maximum biomass as that attained when the same hourly flux was added continuously. Compared with the control, there was also a substantial increase in biomass with pulses of only 1 min each hour. These results demonstrate that the hourly average phosphate concentration to which periphyton communities are exposed is paramount in determining P‐limited growth dynamics. 5. Species composition was not significantly different among treatments in each experiment; however, the design was to evaluate monotonic response with increasing phosphorus flux and species diversity may not respond monotonically. The data are therefore preliminary but suggest the need to determine if species diversity is generally lower when there are brief pulses of phosphate. Unlike pulse experiments that mimic lentic situations, nutrient additions were not used to completion and species success and composition was more dependent on their ability to acquire limiting nutrients rapidly rather than on their ability to take up nutrients at the lowest concentration.