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11.
MARK A. JAMES SONIA MONTENEGRO-JAMES CAROL FAJFAR-WHETSTONE FEDERICO MONTEALEGRE JAMES ERICKSON MIODRAG RISTIC 《The Journal of eukaryotic microbiology》1987,34(3):328-332
Antigenic similarities between Plasmodium and Babesia parasites of the phylum Apicomplexa have been previously demonstrated primarily by the serological cross reactivity observed in the indirect fluorescent antibody (IFA) test. We have now studied the antigenic relationship between the human malaria parasite, Plasmodium falciparum, and the hemoparasitic agent of cattle, Babesia bovis, using rabbit monospecific antibodies produced against individual culture-derived P. falciparum polypeptides and bovine polyspecific antibodies to B. bovis exoantigens. These respective antibodies were found to be distinctly cross reactive in the IFA test using infected erythrocytes (squirrel monkey—P. falciparum; bovine—B. bovis) as antigen substrates. Immunofluorescence was shown to be highly specific for parasite surfaces. Additionally, the degree of reactivity with soluble exoantigens contained in Plasmodium and Babesia culture supernatants was monitored by a two-site enzyme immunoassay employing the cross-reactive antibodies. Further evidence for antigenic cross reactivity between P. falciparum and B. bovis parasites was shown with the in vitro inhibition assay. Antibodies to P. falciparum and B. bovis were found to be highly inhibitory for the in vitro growth of P. falciparum in human erythrocytes. 相似文献
12.
The Gomphaeschninae (Odonata: Aeshnidae): new fossil genus, reconstructed phylogeny, and geographical history 总被引:2,自引:0,他引:2
ABSTRACT. Three new species of fossil Anisoptera (dragonflies) are reported from Tiffanian (Late Palaeocene) sediments of the Paskapoo Formation, near Red Deer, Alberta, Canada. The three species are all assigned to the new genus Alloaeschna , which is classified in the subfamily Gomphaeschninae.
Phylogenetic analysis of gomphaeschnine wing venation suggests that the subfamily is paraphyletic, with derived gomphaeschnines such as Boyeria and Linaeschna more closely related to the Brachytroninae and Aeshninae than are other gomphaeschnines. One of the most primitive gomphaeschnines, and the oldest fossil form, is the Jurassic Morbaeschna. Parallelism, convergence and reversal in the evolution of features of the wing venation is common.
The new genus Alloaeschna is at about the same evolutionary grade as the old-world genus Oligoaeschna and the new-world genus Gomphaeschna , and is one of the more primitive known members of the Gomphaeschninae. The new species are the first recorded Palaeocene gomphaeschnines and the oldest known gomphaeschnines from the Americas.
Several extant genera have fossil representatives on continents different from those on which they now survive, suggesting repeated crossing of land bridges and/or widespread ancestral species prior to the separation of the continents, and subsequent extinction leading to present relict distributions. 相似文献
Phylogenetic analysis of gomphaeschnine wing venation suggests that the subfamily is paraphyletic, with derived gomphaeschnines such as Boyeria and Linaeschna more closely related to the Brachytroninae and Aeshninae than are other gomphaeschnines. One of the most primitive gomphaeschnines, and the oldest fossil form, is the Jurassic Morbaeschna. Parallelism, convergence and reversal in the evolution of features of the wing venation is common.
The new genus Alloaeschna is at about the same evolutionary grade as the old-world genus Oligoaeschna and the new-world genus Gomphaeschna , and is one of the more primitive known members of the Gomphaeschninae. The new species are the first recorded Palaeocene gomphaeschnines and the oldest known gomphaeschnines from the Americas.
Several extant genera have fossil representatives on continents different from those on which they now survive, suggesting repeated crossing of land bridges and/or widespread ancestral species prior to the separation of the continents, and subsequent extinction leading to present relict distributions. 相似文献
13.
14.
Ferri- and Ferrocyanide Salts Change the Current/Voltage Relations of Chara corallina: No Correlation with the Transmembrane Redox System 总被引:1,自引:0,他引:1
The current/voltage {I/V) relations of the plasma membrane ofChara corallina cells are characteristic when the bathing mediumhas elevated (5-0 mM) concentrations of potassium Addition ofeither 0-5 mM femcyanide or 0-5 mM ferrocyanide usually induceda qualitatively similar increase in the leak current,with a concomitant increase in membrane conductance. Both redoxreactants failed for unknown reasons to affect the I/V profileof some of the cells tested In the sensitive cells, femcyanidewas unable to generate extra current over that found upon additionof ferrocyanide Because the feiTocyanide oxidation rate of thecells is only 10% of the femcyanide reduction rate, we concludethat both forms, fern- and ferrocyanide, affect the 'leak' conductanceindependent of the redox state of the reactants, i e ferrocyanidedoes not act indirectly via an oxidation to femcyanide Hence,under the experimental conditions, we were unable to detecta current that could be assigned to the operation of a transmembraneredox system Furthermore, the fern- and ferrocyanide inducedshift in the I/V profile only reversed slowly after withdrawalof the redox reactants. This suggests that the elicited currentis independent of the presence of an extracellular electronacceptor, i.e on the continuous operation of a proposed transmembraneredox system Key words: Current/voltage curves, transmembrane reductase, voltage clamp, femcyanide, ferrocyanide, Chara 相似文献
15.
The roots and mycorrhizas of herbaceous woodland plants 总被引:4,自引:0,他引:4
16.
PATRICIA A. CHAMBERS JOHN MARK HANSON † JANICE M. BURKE ELLIE E. PREPAS 《Freshwater Biology》1990,24(1):81-91
SUMMARY. 1. The impact of crayfish on the biomass, density and shoot morphology of four submersed plant species was examined under semi-natural conditions. Male or female crayfish ( Orconectes virilis ) were held for 5 weeks at biomasses of 0, 5, 10 or 18 g m−2 (live weight) in twelve plastic pools (4.67 m2 , surface area) containing Potamogeton richardsonii, Myriophyllum exalbescens, Nuphar variegatum and Sparganium eurycarpum .
2. Crayfish significantly affected biomass, density and/or shoot morphology of all four macrophyte species. Differences in the effect of crayfish on macrophyte growth were related to plant species, crayfish sex and activity, and the abundance of alternative foods.
3. The effect of female crayfish on macrophyte growth was generally stimulatory. Myriophyllum and Potamogeton biomass, Potamogeton density and Myriophyllum length increased in the presence of female crayfish, possibly due to the reduction in herbivorous snails as a result of crayfish predation. In contrast, plant growth decreased in the presence of male crayfish: Myriophyllum, Nuphar and Potamogeton biomass, Myriophyllum and Sparganium density, and Sparganium and Poiamogeton length were reduced at male crayfish biomasses between 5 and 18 g m−2 .
4. These results indicate that even relatively low densities of crayfish can greatly affect the growth of submersed aquatic plants. Because of their ability to modify aquatic macrophyte, macroinvertebrate and, ultimately, fish communities, the introduction of crayfish into lakes where they do not occur could have a major effect on the structure and composition of the littoral zone. 相似文献
2. Crayfish significantly affected biomass, density and/or shoot morphology of all four macrophyte species. Differences in the effect of crayfish on macrophyte growth were related to plant species, crayfish sex and activity, and the abundance of alternative foods.
3. The effect of female crayfish on macrophyte growth was generally stimulatory. Myriophyllum and Potamogeton biomass, Potamogeton density and Myriophyllum length increased in the presence of female crayfish, possibly due to the reduction in herbivorous snails as a result of crayfish predation. In contrast, plant growth decreased in the presence of male crayfish: Myriophyllum, Nuphar and Potamogeton biomass, Myriophyllum and Sparganium density, and Sparganium and Poiamogeton length were reduced at male crayfish biomasses between 5 and 18 g m
4. These results indicate that even relatively low densities of crayfish can greatly affect the growth of submersed aquatic plants. Because of their ability to modify aquatic macrophyte, macroinvertebrate and, ultimately, fish communities, the introduction of crayfish into lakes where they do not occur could have a major effect on the structure and composition of the littoral zone. 相似文献
17.
SUMMARY.
- 1 We tracked calcite saturation and seston composition during 1987 and 1988 in a shallow, hardwater prairie lake, 1 year before and 1 year after a lake-wide fish removal. We also measured the contribution of calcite to turbidity during 1988.
- 2 In both years calcite saturation increased rapidly after all ice had thawed and peaked during mid-late summer with the mineral saturation index of calcite (SI) sometimes exceeding 30.
- 3 Removal of calcite from lake seston by gentle acidification of unfiltered water samples showed suspended calcite to be an important source of lurbidity in this lake.
- 4 The lake-wide fish removal produced detectable changes in the annual cycle of calcite saturation and precipitation. Increased grazing by Daphnia galeata and Daphnia pulex apparently reduced calcite saturation during early summer by suppressing the phytoplankton, and lowering the demand for CO2.
- 5 Lower calcite precipitation, as well as direct removal of calcite by Daphnia grazing, probably contributed to the improvement in water transparency observed after the fish kill.
18.
MARK F. WISER 《The Journal of eukaryotic microbiology》1995,42(6):659-664
ABSTRACT. Protein phosphorylation events may play important roles in the replication and differentiation of the malarial parasite. Investigations into the lability of a Plasmodium protein kinase revealed that a 34 kDa parasite phosphoprotein is rapidly converted into a 19 kDa fragment. Coincident with this conversion is a nearly total loss of a protein kinase activity, as determined from the phosphorylation of endogenous protein substrates. Both the conversion of the 34 kDa protein to the 19 kDa protein and the loss of protein kinase activity are inhibited by thio-protease inhibitors. The presence of low levels of the intact 34 kDa protein restores the protein kinase activity to almost maximum levels. However, it was not possible to demonstrate protein kinase activity associated with the 34 kDa protein, thus suggesting that the 34 kDa protein is probably an activator or regulator of the protein kinase activity and not a protein kinase. The conversion to the 19 kDa fragment also occurs in vivo and only during the schizont stage prior to the appearance of ring forms. During this same period the protein kinase activity decreases suggesting that the proteolytic processing of the 34 kDa protein may be a physiological regulator of the protein kinase. 相似文献
19.
Recurrent response patterns of a zooplankton community to whole-lake fish manipulation 总被引:2,自引:0,他引:2
XI HE ‡ MARK D. SCHEURELL PATRICIA A. SORANNO RUSSELL A. WRIGHT † 《Freshwater Biology》1994,32(1):61-72
1. In a series of whole-lake manipulations conducted from 1984 to 1991, planktivorous fishes were alternately removed and restocked in a small mesotrophic lake, resulting in dramatic changes in the zooplankton community. 2. Response patterns in the zooplankton community, which include species and size structure, and within-year community variability, were examined. Variation in the zooplankton community in unmanipulated years was much lower than that in manipulated years, regardless of direction of the manipulation (i.e. decreasing or increasing planktivory). 3. The succession of zooplankton species abundance was repeated in the second removal of planktivorous fishes. The community shifted from small-bodied cladocerans, copepods and rotifers, through an intermediate state with high abundance of Holopedium, to an assemblage dominated by large-bodied daphnids. 相似文献
20.
MARK A. FARMER 《The Journal of eukaryotic microbiology》1993,40(5):619-626
ABSTRACT. Ditrichomonas honigbergii n. g., n. sp. is a small trichomonad flagellate that has three emergent flagella arising from four basal bodies, a parabasal apparatus (single dictyosome with associated striated flagellar rootlets), a microtubular axostyle, a short undulating membrane, and hydrogenosomes. Cultures of D. honigbergii were isolated from the sediments of a freshwater lake and there is no known metazoan host. Cells form walled cysts with internalized flagella and go through all phases of the life cycle (excystment, binary division, encystment) without any perturbations to the culture medium. Ditrichornonas honigbergii is capable of ingesting and digesting bacteria by phagocytosis. These facts suggest that D. honigbergii may be a free-living inhabitant of oxygen-reduced environments. The structure of D. honigbergii is similar to that of retortamonads and the relationship of trichomonads to other amitochondrial protists is discussed. 相似文献