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821.
ERICA LASEK-NESSELQUIST DAVID MARK WELCH RICHARD CHRISTOPHER ANDREW THOMPSON ROBERT F. STEUART MITCHELL L. SOGIN 《The Journal of eukaryotic microbiology》2009,56(6):504-518
ABSTRACT. Meiotic sex evolved early in the history of eukaryotes. Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis ), a parasitic protist belonging to an early diverging lineage of eukaryotes, shows no cytological or physiological evidence of meiotic or sexual processes. Recent molecular analyses challenge the idea that G. duodenalis is a strictly clonal organism by providing evidence of recombination between homologous chromosomes within one subgroup (Assemblage A) of this species as well as genetic transfer from one subgroup to another (Assemblage A–B). Because recombination is not well documented and because it is not known whether the observed inter-assemblage transfer represents true reciprocal genetic exchange or a non-sexual process, we analyzed genic sequences from all major subgroups (Assemblages A–G) of this species. For all assemblages, we detected molecular signatures consistent with meiotic sex or genetic exchange, including low levels of heterozygosity, as indicated by allelic sequence divergence within isolates, and intra- and inter-assemblage recombination. The identification of recombination between assemblages suggests a shared gene pool and calls into question whether it is appropriate to divide the genetically distinct assemblages of G. duodenalis into a species complex. 相似文献
822.
CATHERINE I. CULLINGHAM MEDEA CURTEANU MARK C. BALL MICHELINE MANSEAU 《The Journal of wildlife management》2010,74(4):849-859
Abstract: Genetic profiling using fecal samples collected noninvasively in the wild can provide managers with an alternative to live-trapping. However, before embarking on a large-scale survey, feasibility of this methodology should be assessed for the focal species. Costs associated with fecal genotyping can be high because of the need for multiple amplifications to prevent and detect errors. Assessing the relative amount of target DNA before genotyping means samples can be eliminated where error rates will be high or amplification success will be low, thereby reducing costs. We collected fecal samples from an endangered population of swift fox (Vulpes velox) and employed target-DNA quantification and a screening protocol to assess sample quality before genetic profiling. Quantification was critical in identifying samples of low quality (68%, <0.2 ng/μL). Comparison of the amplification, from a subset of loci in 25 samples that did not meet the screening criteria, confirmed the effectiveness of this method. The protocol, however, used a considerable amount of DNA, and an assessment of the locus and sample variability allowed us to refine it for future population surveys. Although we did not use <50% of the samples we collected, the remaining samples provided 36 unique genotypes, which corresponded to approximately 70% of animals estimated to be present in the study area. Although obtaining fecal DNA from small carnivores is challenging, our protocol, including the quantification and qualification of DNA, the selection of markers, and the use of postgenotyping analyses, such as DROPOUT, CAPWIRE, and geographic information, provides a more cost-effective way to produce reliable results. The method we have developed is an informative approach that wildlife managers can use to conduct population surveys where the collection of feces is possible without the need for live-trapping. 相似文献
823.
The sugarbeet root maggot Tetanops myopaeformis Röder (Diptera: Ulidiidae) can be stored in moist sand at 4–6 °C for up to 5 years and is freeze‐tolerant. The majority of stored larvae survive in a state of post‐diapause quiescence and the remainder are in a multi‐year diapause. The present study aims to determine larval water content and water loss rates in diapausing and low‐temperature stored larvae. Body water content ranges from 57% to 70.1%. Two distinct groupings of larvae are revealed based on dry weights. The first group consists of the diapausing larvae and larvae stored for 1 year. This group has significantly higher dry weights than the second grouping, which consists of the larvae stored for 2 and 3 years. There are no significant differences within each group. Larval water losses follow a first‐order kinetic relationship with time. Larvae stored for 2 years lose water at a significantly higher rate than diapausing larvae. Larvae exhibit no active water uptake at storage temperatures. A freezing event does not induce a significant decrease in wet weights, nor does it increase larval water loss rates. These results indicate that metabolic water and the microclimate during storage are key factors enabling the long‐term survival of T. myopaeformis larvae during low‐temperature storage, and may provide insights for maintaining other insect species under similar conditions. 相似文献
824.
KIRSTEN L. MANDUELL MARK E. HARRISON SUSANNAH K. S. THORPE 《American journal of primatology》2012,74(12):1128-1142
The influence of habitat structure and support availability on support use is an important aspect of understanding locomotor behavior in arboreal primates. We compared habitat structure and support availability in three orangutan study sites—two on Sumatra (Pongo abelii) in the dry‐lowland forest of Ketambe and peat swamp forest of Suaq Balimbing, and one on Borneo (Pongo pygmaeus wurmbii) in the disturbed peat swamp forest of Sabangau—to better understand orangutan habitat use. Our analysis revealed vast differences in tree and liana density between the three sites. Sabangau had a much higher overall tree density, although both Sumatran sites had a higher density of larger trees. The two peat swamp forests were more similar to each other than to Ketambe, particularly with regard to support availability. Ketambe had a wider variety of supports of different sizes and types, and a higher density of larger lianas than the two peat swamps. Orangutans in all three sites did not differ substantially in terms of their preferred supports, although Sumatran orangutans had a strong tendency to use lianas, not observed in Sabangau. Differences in observed frequencies of locomotor behavior suggest the homogeneous structure of Sabangau limits the locomotor repertoire of orangutans, with high frequencies of fewer behaviors, whereas the wider range of supports in Ketambe appears to have facilitated a more varied locomotor repertoire. There were no differences among age‐sex classes in the use of arboreal pathways in Suaq Balimbing, where orangutans selected larger trees than were typically available. This was less apparent in Sabangau, where orangutans generally used trees in relation to their environmental abundance, reflecting the homogeneous nature of disturbed peat swamp forest. These results demonstrate that forest architecture has an important influence on orangutan locomotion, which may become increasingly important as the structure of orangutan habitat continues to be altered through human disturbance. Am. J. Primatol. 74:1128‐1142, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
825.
826.
One and the same: integrative taxonomic evidence that Bactrocera invadens (Diptera: Tephritidae) is the same species as the Oriental fruit fly Bactrocera dorsalis
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MARK K. SCHUTZE KHALID MAHMOOD ANA PAVASOVIC WANG BO JAYE NEWMAN ANTHONY R. CLARKE MATTHEW N. KROSCH STEPHEN L. CAMERON 《Systematic Entomology》2015,40(2):472-486
The invasive fruit fly Bactrocera invadens Drew, Tsuruta & White, and the Oriental fruit fly Bactrocera dorsalis (Hendel) are highly destructive horticultural pests of global significance. Bactrocera invadens originates from the Indian subcontinent and has recently invaded all of sub‐Saharan Africa, while B. dorsalis principally occurs from the Indian subcontinent towards southern China and South‐east Asia. High morphological and genetic similarity has cast doubt over whether B. invadens is a distinct species from B. dorsalis. Addressing this issue within an integrative taxonomic framework, we sampled from across the geographic distribution of both taxa and: (i) analysed morphological variation, including those characters considered diagnostic (scutum colour, length of aedeagus, width of postsutural lateral vittae, wing size, and wing shape); (ii) sequenced four loci (ITS1, ITS2, cox1 and nad4) for phylogenetic inference; and (iii) generated a cox1 haplotype network to examine population structure. Molecular analyses included the closely related species, Bactrocera kandiensis Drew & Hancock. Scutum colour varies from red‐brown to fully black for individuals from Africa and the Indian subcontinent. All individuals east of the Indian subcontinent are black except for a few red‐brown individuals from China. The postsutural lateral vittae width of B. invadens is narrower than B. dorsalis from eastern Asia, but the variation is clinal, with subcontinent B. dorsalis populations intermediate in size. Aedeagus length, wing shape and wing size cannot discriminate between the two taxa. Phylogenetic analyses failed to resolve B. invadens from B. dorsalis, but did resolve B. kandiensis. Bactrocera dorsalis and B. invadens shared cox1 haplotypes, yet the haplotype network pattern does not reflect current taxonomy or patterns in thoracic colour. Some individuals of B. dorsalis/B. invadens possessed haplotypes more closely related to B. kandiensis than to conspecifics, suggestive of mitochondrial introgression between these species. The combined evidence fails to support the delimitation of B. dorsalis and B. invadens as separate biological species. Consequently, existing biological data for B. dorsalis may be applied to the invasive population in Africa. Our recommendation, in line with other recent publications, is that B. invadens be synonymized with B. dorsalis. 相似文献
827.
JOHN G. IRONS III † MARK W. OSWOOD R. JEAN STOUT‡ CATHY M. PRINGLE§ 《Freshwater Biology》1994,32(2):401-411
1. Forest stream food webs depend largely on input of dead riparian zone leaves for their energy, which is converted into living biomass by microbes, macroinvertebrates and fish. 2. Temperature has been invoked as important in controlling breakdown rates, and aquatic biologists have suggested that by normalizing processing rates to degree days rather than days, one can ‘factor out’ the effect of temperature and compare processing rates in streams with different thermal regimes (e.g. different seasons or study sites in different biomes). 3. We examined processing rates (k) along a latitudinal (i.e. thermal) gradient by using reciprocal transplants of leafpacks. We placed leafpacks of ten tree species (representing a large range of leaf litter quality) in streams in Costa Rica, Michigan and Alaska using coarse-mesh (20mm) litter bags. We then examined both the ‘per day’(kday) and ‘per degree day’ (kdegree day) models of leaf litter processing. While processing rates (per day) were fastest at the Costa Rica site (as expected), rates at the Alaska and Michigan sites were similar to each other, which we would not predict if temperature were the principal factor controlling breakdown rate. If using degree days eliminates any effect of differing thermal regimes, rates should be similar across latitudes; however, rates at the Alaska site were much faster (per degree day) than rates at the sites in Costa Rica and Michigan. 4. We compared our data with studies in the North American literature. Regression analysis of kday and kdegree day against latitude of the study site revealed that processing rates (kday) of leaves (from a wide range of tree species in a wide range of stream types) showed no significant change with increasing latitude. However, when normalized for temperature (kDegree day), a positive correlation was found between processing rates and latitude, causing us to reject the hypothesis that normalizing processing rates to cumulative degree days removes the effect of temperature. 5. We suggest three hypotheses: (i) shredding insect populations have adapted to the local thermal regime, and invertebrate-mediated processing rates are either similar between regions (showing no latitudinal pattern), or increase with latitude; (ii) microbial populations are less active at colder temperatures, and the rate of microbially mediated processing of leaf litter will show a decrease with latitude, and consequently (iii) the relative importance of invertebrate v microbial processing changes on a latitudinal gradient, with invertebrates being more important at high latitudes. 相似文献
828.
829.
Results of 3 years of pollen trapping on Barro Colorado Island, Panama, are presented. Minimum pollen dispersal distances are estimated for the most abundant pollen taxa. Dispersal distances for some taxa appear to be as low as 5 m, while for other taxa at least 50% of the trapped pollen had travelled more than 40 m. No consistent pattern of spatial variation was reflected in the pollen rain, however, samples close to large canopy gaps had exceptional abundances of 'disturbance' taxa. A preliminary analysis of the representation of canopy components indicates that as much as 19% of pollen caught in the traps was derived from large tree species. 相似文献
830.
1. Insects commonly resist parasites using melanotic encapsulation. Many studies measuring immune response use the amount of melanin deposited on an artificial object that has been inserted into the animal as a proxy of the amount of resistance that the host is capable of mounting to natural parasites. 2. The relevance of this methodology to immune response in natural insect populations needs further study. Here, we examined two temperate damselfly species to elucidate the relationships among damselfly size, natural resistance to mites, and the immune response mounted by the same damselflies against nylon filaments. 3. The damselfly species that had high rates of melanotic encapsulation of mites in nature deposited more melanin on the nylon inserts than the species with low rates of natural resistance. 4. In females of this species, those that had resisted mites naturally melanised the nylon filament more aggressively than those that did not resist mites. 5. Our results show some support for the use of nylon filaments to assess natural patterns of immune response in these damselflies, but also suggest that caution should be used in interpreting the responses. 相似文献